全文获取类型
收费全文 | 989篇 |
免费 | 77篇 |
专业分类
1066篇 |
出版年
2022年 | 15篇 |
2021年 | 15篇 |
2020年 | 9篇 |
2019年 | 9篇 |
2018年 | 16篇 |
2017年 | 18篇 |
2016年 | 27篇 |
2015年 | 43篇 |
2014年 | 42篇 |
2013年 | 47篇 |
2012年 | 71篇 |
2011年 | 70篇 |
2010年 | 41篇 |
2009年 | 39篇 |
2008年 | 43篇 |
2007年 | 44篇 |
2006年 | 54篇 |
2005年 | 42篇 |
2004年 | 43篇 |
2003年 | 39篇 |
2002年 | 37篇 |
2001年 | 12篇 |
2000年 | 7篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 10篇 |
1996年 | 15篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1987年 | 8篇 |
1985年 | 10篇 |
1984年 | 7篇 |
1983年 | 12篇 |
1981年 | 6篇 |
1980年 | 8篇 |
1979年 | 7篇 |
1977年 | 8篇 |
1976年 | 10篇 |
1975年 | 6篇 |
1972年 | 6篇 |
1971年 | 5篇 |
1969年 | 8篇 |
1968年 | 9篇 |
1967年 | 5篇 |
1962年 | 6篇 |
1961年 | 6篇 |
1958年 | 5篇 |
排序方式: 共有1066条查询结果,搜索用时 0 毫秒
71.
Emma Torrecillas Maria del Mar Alguacil Antonio Roldán Gisela Díaz Alicia Montesinos-Navarro Maria Pilar Torres 《Applied and environmental microbiology》2014,80(17):5457-5466
Patterns in plant–soil biota interactions could be influenced by the spatial distribution of species due to soil conditions or by the functional traits of species. Gypsum environments usually constitute a mosaic of heterogeneous soils where gypsum and nongypsum soils are imbricated at a local scale. A case study of the interactions of plants with arbuscular mycorrhizal fungi (AMF) in gypsum environments can be illustrative of patterns in biotic interactions. We hypothesized that (i) soil characteristics might affect the AMF community and (ii) there are differences between the AMF communities (modules) associated with plants exclusive to gypsum soils (gypsophytes) and those associated with plants that show facultative behavior on gypsum and/or marly-limestone soils (gypsovags). We used indicator species and network analyses to test for differences between the AMF communities harbored in gypsophyte and gypsovag plants. We recorded 46 operational taxonomic units (OTUs) belonging to nine genera of Glomeromycota. The indicator species analysis showed two OTUs preferentially associating with gypsum soils and three OTUs preferentially associating with marly-limestone soils. Modularity analysis revealed that soil type can be a major factor shaping AMF communities, and some AMF groups showed a tendency to interact differently with plants that had distinct ecological strategies (gypsophytes and gypsovags). Characterization of ecological networks can be a valuable tool for ascertaining the potential influence of above- and below-ground biotic interactions (plant-AMF) on plant community composition. 相似文献
72.
Juan José Ortiz Guzmán Arcos‐García José Luis Germán D. Mendoza Martínez Fernando Xicoténcatl Plata Pérez Gisela Fuentes Mascorro Gabriela Ruelas Inzunza 《Zoo biology》2013,32(3):277-280
The effects of the addition of lysine to commercial feed given to captive black iguana (Ctenosaura pectinata) were evaluated in terms of growth and feed digestibility. Twenty‐eight‐day‐old black iguana with an initial weight of 5.5 ± 0.3 g were housed individually in cages measuring 45 × 45 × 45 cm. The experiment lasted 150 days. The ambient temperature ranged from 28 to 35°C with a relative humidity of 60 to 95%. Treatments consisted of the addition of different percentages of lysine to the feed (0.0, 0.1, 0.2, and 0.3%, dry matter [DM] base). There was a linear response (P < 0.01) in daily gain (68, 112, 118, and 151 mg/d) and daily intake (251, 289, 297, and 337 mg/d) for levels from 0 to 0.3%, respectively, as well in the growth in head size, snout–vent length, and total length. The digestibility of DM, neutral detergent fiber, and acid detergent fiber were reduced linearly (P < 0.01) as lysine levels increased. Intake and digestibility were negatively correlated (r = –0.74; P < 0.001). It is concluded that the addition of lysine to the black iguana diet in the first months of life is important to stimulate growth and intake. Zoo Biol 32:277–280, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
73.
Jonathan A. Coles Gisela Schneider-Picard 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,165(1):109-118
Summary Photoreceptor cells of the drone,Apismellifera , have a voltage-gated Na+ membrane conductance that can be blocked by tetrodotoxin (TTX) and generates an action potential on abrupt depolarization: an action potential is triggered by the rising phase of a receptor potential evoked by an intense light flash (Autrum and von Zwehl 1964; Baumann 1968). We measured the intracellular voltage response to a small (9%), brief (30 ms) decrease in light intensity from a background, and found that its amplitude was decreased by 1M TTX. The response amplitude was maximal when the background intensity depolarized the cell to –38 mV. With intensities depolarizing the cell membrane to –45 to –33 mV the average response amplitude was decreased by TTX from 1.2mV to 0.5mV. TTX is also known to decrease the voltage noise during steady illumination (Ferraro et al. 1983) but, despite this, the ratio of peak-to-peak signal to noise was, on average, decreased by TTX. The results suggest that drone photoreceptors use voltage-gated Na+ channels for graded amplification of responses to small, rapid changes in light intensity.Abbreviations
TTX
tetrodotoxin
-
V
i
intracellular potential with respect to the bath
-
V
o
extracellular potential
-
V
m,V
i-V
o
approximate transmembrane potential
-
S
amplitude of the voltage response to an 8.9% decrease in light intensity
-
N
voltage noise, usually measured as root mean square voltage deviation as described in Methods 相似文献
74.
75.
Gisela Schlosser 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1958,28(5):217-223
Zusammenfassung Eine Zusammenstellung von Ergebnissen zeigt, daß es weitestgehend gelungen ist, einen cumarinarmen Steinklee zu züchten. Es wird die Untersuchungsmethodik in qualitativer und quantitativer Hinsicht beschrieben und auf das Auftreten von Wechseltypen innerhalb des süßen Zuchtmaterials hingewiesen, denen besondere Beachtung im Rahmen der Züchtung geschenkt werden muß.Was die Untersuchungen des Blatt- und Stengel-anteils der verschiedenen Arten betrifft, so tragen diese Ergebnisse einen vorläufigen Charakter.Mit 13 Abbildungen 相似文献
76.
María C. Tellería Gisela Sancho Vicky A. Funk Iralys Ventosa Nadia Roque 《Plant Systematics and Evolution》2013,299(5):935-948
In the context of recent molecular phylogenies of the basal grades of Compositae, we investigated the utility of pollen morphology within the tribe Gochnatieae. The pollen of 64 species of Anastraphia, Cnicothamnus, Cyclolepis, Gochnatia, Pentaphorus, and Richterago was studied using light microscopy and scanning electron microscopy. In addition, three extra-Gochnatieae genera (Ianthopappus, Leucomeris, and Nouelia) were examined as they were traditionally morphologically related to members of the tribe Gochnatieae. Three of the species of Gochnatieae were examined using transmission electron microscopy. Two pollen types, and two new subtypes, have been recognized on the basis of the pollen shape, size, and exine sculpture. The pollen features of Gochnatia sect. Moquiniastrum and G. cordata are similar and distinctive within the genus and support the recently re-circumscribed section Hedraiophyllum. Within the species with echinate pollen surface, the distinctive spine length of Anastraphia supports its recent resurrection as a genus. The identity of Pentaphorus could not be supported by pollen features as was for other morphological characteristics. The pollen features shared across Cyclolepis, Ianthopappus, Leucomeris, Nouelia and Gochnatia sect. Moquiniastrum, as well as those shared by Richterago and Anastraphia could be a result of parallel evolution. 相似文献
77.
Gisela R. Franchini Lucrecia M. Curto Julio J. Caramelo José María Delfino 《Protein science : a publication of the Protein Society》2009,18(12):2592-2602
A lingering issue in the area of protein engineering is the optimal design of β motifs. In this regard, the framework provided by intestinal fatty acid binding protein (IFABP) was successfully chosen to explore the consequences on structure and function of the redesign of natural motifs. A truncated form of IFABP (Δ98Δ) served to illustrate the nonintuitive notion that the integrity of the β‐barrel can indeed be compromised with no effect on the ability to attain a native‐like fold. This is most likely the outcome of the key role played by the preservation of essential core residues. In the search for the minimal structural determinants of this fold, Δ98Δ offered room for further intervention. A dissection of this protein leads to a new abridged variant, Δ78Δ, containing 60% of the amino acids of IFABP. Spectroscopic analyses indicate that Δ78Δ retains substantial β‐sheet content and preserves tertiary interactions, displaying cooperative unfolding and binding activity. Most strikingly, this construct adopts a remarkably stable dimeric structure in solution. This phenomenon takes advantage of the inherent structural plasticity of this motif, likely profitting from edge‐to‐edge interactions between β‐sheets, whereas avoiding the most commonly occurring outcome represented by aggregation. 相似文献
78.
Mackey AL Andersen LL Frandsen U Suetta C Sjøgaard G 《Journal of applied physiology (Bethesda, Md. : 1985)》2010,109(6):1920-1929
It is hypothesized that repeated recruitment of low-threshold motor units is an underlying cause of chronic pain in trapezius myalgia. This study investigated the distribution of satellite cells (SCs), myonuclei, and macrophages in muscle biopsies from the trapezius muscle of 42 women performing repetitive manual work, diagnosed with trapezius myalgia (MYA; 44 ± 8 yr; mean ± SD) and 20 matched healthy controls (CON; 45 ± 9 yr). Our hypothesis was that muscle of MYA, in particular type I fibers, would demonstrate higher numbers of SCs, myonuclei, and macrophages compared with CON. SCs were identified on muscle cross sections by combined immunohistochemical staining for Pax7, type I myosin, and laminin, allowing the number of SCs associated with type I and II fibers to be determined. We observed a pattern of SC distribution in MYA previously only reported for individuals above 70 yr of age. Compared with CON, MYA demonstrated 19% more SCs per fiber associated with type I fibers (MYA 0.098 ± 0.039 vs. CON 0.079 ± 0.031; P < 0.05) and 40% fewer SCs associated with type II fibers (MYA 0.047 ± 0.017 vs. CON 0.066 ± 0.035; P < 0.05). The finding of similar numbers of macrophages between the two groups was not in line with our hypothesis and suggests that the elevated SC content of MYA was not due to heightened inflammatory cell contents, but rather to provide new myonuclei. The findings of greater numbers of SCs in type I fibers of muscle subjected to repeated low-intensity work support our hypothesis and provide new insight into stimuli capable of regulating SC content. 相似文献
79.
Roussel A Lescar J Vaney MC Wengler G Wengler G Rey FA 《Structure (London, England : 1993)》2006,14(1):75-86
Semliki Forest virus (SFV) is enveloped by a lipid bilayer enclosed within a glycoprotein cage made by glycoproteins E1 and E2. E1 is responsible for inducing membrane fusion, triggered by exposure to the acidic environment of the endosomes. Acidic pH induces E1/E2 dissociation, allowing E1 to interact with the target membrane, and, at the same time, to rearrange into E1 homotrimers that drive the membrane fusion reaction. We previously reported a preliminary Calpha trace of the monomeric E1 glycoprotein ectodomain and its organization on the virus particle. We also reported the 3.3 A structure of the trimeric, fusogenic conformation of E1. Here, we report the crystal structure of monomeric E1 refined to 3 A resolution and describe the amino acids involved in contacts in the virion. These results identify the major determinants for the E1/E2 icosahedral shell formation and open the way to rational mutagenesis approaches to shed light on SFV assembly. 相似文献
80.