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11.
L. Mariniello R. Porta A. Sorrentino C. V. L. Giosafatto G. Rossi Marquez M. Esposito P. Di Pierro 《Amino acids》2014,46(3):767-776
Various strategies have been explored in the last 20 years to modify the functional properties of proteins and, among these, protein/polymer conjugation resulted one of the most successful approaches. Thus, the surface modification of polypeptides of potential industrial interest by covalent attachment of different macromolecules is nowadays regarded as an extremely valuable technique to manipulate protein activities. Protein derivatives with a number of either natural or synthetic polymers, like different polysaccharides or polyethylene glycol, have been obtained by both chemical and enzymatic treatments, and in this context, the crosslinking enzyme transglutaminase is attracting an increasing attention as a simple and safe means for protein processing in vitro. In this short review, we summarized the most significant experimental findings demonstrating that a microbial form of the enzyme is an effective tool to obtain several biopolymer-based conjugates potentially useful for both food and pharmaceutical applications. 相似文献
12.
TP Souza-Junior L Loren?o-Lima D Ganini CV Vardaris TG Polotow MP Barros 《Biology of sport / Institute of Sport》2014,31(4):271-276
Reactive oxygen species are produced during anaerobic exercise mostly by Fe ions released into plasma and endothelial/muscle xanthine oxidase activation that generates uric acid (UA) as the endpoint metabolite. Paradoxically, UA is considered a major antioxidant by virtue of being able to chelate pro-oxidative iron ions. This work aimed to evaluate the relationship between UA and plasma markers of oxidative stress following the exhaustive Wingate test. Plasma samples of 17 male undergraduate students were collected before, 5 and 60 min after maximal anaerobic effort for the measurement of total iron, haem iron, UA, ferric-reducing antioxidant activity in plasma (FRAP), and malondialdehyde (MDA, biomarker of lipoperoxidation). Iron and FRAP showed similar kinetics in plasma, demonstrating an adequate pro-/antioxidant balance immediately after exercise and during the recovery period (5–60 min). Slight variations of haem iron concentrations did not support a relevant contribution of rhabdomyolysis or haemolysis for iron overload following exercise. UA concentration did not vary immediately after exercise but rather increased 29% during the recovery period. Unaltered MDA levels were concomitantly measured. We propose that delayed UA accumulation in plasma is an auxiliary antioxidant response to post-exercise (iron-mediated) oxidative stress, and the high correlation between total UA and FRAP in plasma (R-Square = 0.636; p = 0.00582) supports this hypothesis. 相似文献
13.
We refer to Oswaldo Cruz''s reports dating from 1913 about the necessities of a
healthcare system for the Brazilian Amazon Region and about the journey of Carlos
Chagas to 27 locations in this region and the measures that would need to be adopted.
We discuss the risks of endemicity of Chagas disease in the Amazon Region. We
recommend that epidemiological surveillance of Chagas disease in the Brazilian Amazon
Region and Pan-Amazon region should be implemented through continuous monitoring of
the human population that lives in the area, their housing, the environment and the
presence of triatomines. The monitoring should be performed with periodic
seroepidemiological surveys, semi-annual visits to homes by health agents and the
training of malaria microscopists and healthcare technicians to identify
Trypanosoma cruzi from patients'' samples and T.
cruzi infection rates among the triatomines caught. We recommend health
promotion and control of Chagas disease through public health policies, especially
through sanitary education regarding the risk factors for Chagas disease. Finally, we
propose a healthcare system through base hospitals, intermediate-level units in the
areas of the Brazilian Amazon Region and air transportation, considering the
distances to be covered for medical care. 相似文献