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51.
Hernández-Quintero JD MH Reyes-Valdés DV Mendoza-Rodríguez M Gómez-Martínez R Rodríguez-Herrera 《Phyton》2015,84(1):107-112
The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin dye. For the study of meiosis, male flower buds were collected, fixed and stained for analysis with the same dye. As a result, the gametic (n = x = 19) and somatic chromosome (2n = 38) numbers of D. cedrosanum are reported for the first time, being consistent with previous findings in other Dasylirion species, which points to a constant ploidy level across the genus. Variation was observed in the morphology and size of the somatic chromosomes, with types ranging from submetacentric to subtelocentric, and sizes oscillating in a range of 4.43 µm, with an average total length of 112.38 µm for the diploid chromosome complement. This shows that the chromosome complement of D. cedrosanom would belong to a 3B classification of Stebins, with a medium variation between chromosome lengths and low chromosome asymmetry. This variation indicates the feasibility of constructing a chromosome ideotype for this species. The meiotic chromosome pairing showed a chromosome behavior consistent with a disomic inheritance characteristic of a diploid species, with prevalence of ring and chain bivalents, typically without pairing abnormalities. Bivalent configurations in all cases were symmetrical.The normal and symmetrical meiotic pairing indicates a balanced production of gametes, and suggests the absence of heteromorphic sex determination. 相似文献
52.
Rampant horizontal transfer and duplication of rubisco genes in eubacteria and plastids 总被引:14,自引:2,他引:14
Previous work has shown that molecular phylogenies of plastids,
cyanobacteria, and proteobacteria based on the rubisco (ribulose-1,5-
bisphosphate carboxylase/oxygenase) genes rbcL and rbcS are incongruent
with molecular phylogenies based on other genes and are also incompatible
with structural and biochemical information. Although it has been much
speculated that this is the consequence of a single horizontal gene
transfer (of a proteobacterial or mitochondrial rubisco operon into
plastids of rhodophytic and chromophytic algae), neither this hypothesis
nor the alternative hypothesis of ancient gene duplication have been
examined in detail. We have conducted phylogenetic analyses of all
available bacterial rbcL sequences, and representative plastid sequences,
in order to explore these alternative hypothesis and fully examine the
complexity of rubisco gene evolution. The rbcL phylogeny reveals a
surprising number of gene relationships that are fundamentally incongruent
with organismal relationships as inferred from multiple lines of other
molecular evidence. On the order of six horizontal gene transfers are
implied by the form I (L8S8) rbcL phylogeny, two between cyanobacteria and
proteobacteria, one between proteobacteria and plastids, and three within
proteobacteria. Alternatively, a single ancient duplication of the form I
rubisco operon, followed by repeated and pervasive differential loss of one
operon or the other, would account for much of this incongruity. In all
probability, the rubisco operon has undergone multiple events of both
horizontal gene transfer and gene duplication in different lineages.
相似文献
53.
Molecular clocks are routinely tested for linearity using a relative rate
test and routinely calibrated against the geological time scale using a
single or average paleontologically determined time of divergence between
living taxa. The relative rate test is a test of parallel rate equality,
not a test of rate constancy. Temporal scaling provides a test of rates,
where scaling coefficients of 1.0 (isochrony) represent stochastic rate
constancy. The fossil record of primates and other mammals is now known in
sufficient detail to provide several independent divergence times for major
taxonomic groups. Molecular difference should scale negatively or
isochronically (scaling coefficients less than 1.0) with divergence time:
where two or more divergence times are available, molecular difference
appears to scale positively (scaling coefficient greater than 1.0). A
minimum of four divergence times are required for adequate statistical
power in testing the linear model: scaling is significantly nonlinear and
positive in six of 11 published investigations meeting this criterion. All
groups studied show some slowdown in rates of molecular change over
Cenozoic time. The break from constant or increasing rates during the
Mesozoic to decreasing rates during the Cenozoic appears to coincide with
extraordinary diversification of placental mammals at the beginning of this
era. High rates of selectively neutral molecular change may be concentrated
in such discrete events of evolutionary diversification.
相似文献
54.
55.
Raquel Burger-Calderon Victoria Madden Ryan A. Hallett Aaron D. Gingerich Volker Nickeleit Jennifer Webster-Cyriaque 《Journal of virology》2014,88(1):559-573
BK polyomavirus (BKPyV) is the most common viral pathogen among allograft patients. Increasing evidence links BKPyV to the human oral compartment and to HIV-associated salivary gland disease (HIVSGD). To date, few studies have analyzed orally derived BKPyV. This study aimed to characterize BKPyV isolated from throat wash (TW) samples from HIVSGD patients. The replication potential of HIVSGD-derived clinical isolates HIVSGD-1 and HIVSGD-2, both containing the noncoding control region (NCCR) architecture OPQPQQS, were assessed and compared to urine-derived virus. The BKPyV isolates displayed significant variation in replication potential. Whole-genome alignment of the two isolates revealed three nucleotide differences that were analyzed for a potential effect on the viral life cycle. Analysis revealed a negligible difference in NCCR promoter activity despite sequence variation and emphasized the importance of functional T antigen (Tag) for efficient replication. HIVSGD-1 encoded full-length Tag, underwent productive infection in both human salivary gland cells and kidney cells, and expressed viral DNA and Tag protein. Additionally, HIVSGD-1 generated DNase-resistant particles and by far surpassed the replication potential of the kidney-derived isolate in HSG cells. HIVSGD-2 encoded a truncated form of Tag and replicated much less efficiently. Quantitation of infectious virus, via the fluorescent forming unit assay, suggested that HIVSGD BKPyV had preferential tropism for salivary gland cells over kidney cells. Similarly, the results suggested that kidney-derived virus had preferential tropism for kidney cells over salivary gland cells. Evidence of HIVSGD-derived BKPyV oral tropism and adept viral replication in human salivary gland cells corroborated the potential link between HIVSGD pathogenesis and BKPyV. 相似文献
56.
Maeva J. Orliac Sandrine Ladevèze Philip D. Gingerich Renaud Lebrun Thierry Smith 《Proceedings. Biological sciences / The Royal Society》2014,281(1781)
Expansion of the brain is a key feature of primate evolution. The fossil record, although incomplete, allows a partial reconstruction of changes in primate brain size and morphology through time. Palaeogene plesiadapoids, closest relatives of Euprimates (or crown-group primates), are crucial for understanding early evolution of the primate brain. However, brain morphology of this group remains poorly documented, and major questions remain regarding the initial phase of euprimate brain evolution. Micro-CT investigation of the endocranial morphology of Plesiadapis tricuspidens from the Late Palaeocene of Europe—the most complete plesiadapoid cranium known—shows that plesiadapoids retained a very small and simple brain. Plesiadapis has midbrain exposure, and minimal encephalization and neocorticalization, making it comparable with that of stem rodents and lagomorphs. However, Plesiadapis shares a domed neocortex and downwardly shifted olfactory-bulb axis with Euprimates. If accepted phylogenetic relationships are correct, then this implies that the euprimate brain underwent drastic reorganization during the Palaeocene, and some changes in brain structure preceded brain size increase and neocortex expansion during evolution of the primate brain. 相似文献
57.
Andrew James Gingerich David P. Philipp Cory D. Suski 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2010,180(3):371-384
The influence of feeding on swimming performance and exercise recovery in fish is poorly understood. Examining swimming behavior
and physiological status following periods of feeding and fasting is important because wild fish often face periods of starvation.
In the current study, researchers force fed and fasted groups of largemouth bass (Micropterus salmoides) of similar sizes for a period of 16 days. Following this feeding and fasting period, fish were exercised for 60 s and monitored
for swimming performance and physiological recovery. Resting metabolic rates were also determined. Fasted fish lost an average
of 16 g (nearly 12%) of body mass, while force fed fish maintained body mass. Force fed fish swam 28% further and required
nearly 14 s longer to tire during exercise. However, only some physiological conditions differed between feeding groups. Resting
muscle glycogen concentrations was twofold greater in force fed fish, at rest and throughout recovery, although it decreased
in both feeding treatments following exercise. Liver mass was nearly three times greater in force fed fish, and fasted fish
had an average of 65% more cortisol throughout recovery. Similar recovery rates of most physiological responses were observed
despite force fed fish having a metabolic rate 75% greater than fasted fish. Results are discussed as they relate to largemouth
bass starvation in wild systems and how these physiological differences might be important in an evolutionary context. 相似文献
58.
59.
Gingerich PD 《Trends in ecology & evolution》2006,21(5):246-253
The modern orders of mammals, Artiodactyla, Perissodactyla and Primates (APP taxa), first appear in the fossil record at the Paleocene-Eocene boundary, c. 55 million years ago. Their appearance on all three northern continents has been linked to diversification and dispersal in response to rapid environmental change at the beginning of a worldwide 100 000-200 000-year Paleocene-Eocene thermal maximum (PETM) and carbon isotope excursion. As I discuss here, global environmental events such as the PETM have had profound effects on evolution in the geological past and must be considered when modeling the history of life. The PETM is also relevant when considering the causes and consequences of global greenhouse warming. 相似文献
60.
A zebrafish maternal effect mutation, in the gene hecate, results in embryos that have defects in the formation of dorsoanterior structures and altered calcium release. hecate mutant embryos lack nuclear accumulation of beta-catenin and have reduced expression of genes specific to the dorsal organizer. We found that hecate mutant embryos exhibit a nearly 10-fold increase in the frequency of intracellular Ca2+ transients normally present in the enveloping layer during the blastula stages. Inhibition of Ca2+ release leads to ectopic expression of dorsal genes in mutant embryos suggesting that Ca2+ transients are important in mediating dorsal gene expression. Inhibition of Ca2+ release also results in the expression of dorsal-specific genes in the enveloping layer in a beta-catenin-independent manner, which suggests an additional function for the Ca2+ transients in this cellular layer. The mutant phenotype can be reversed by the expression of factors that activate Wnt/beta-catenin signaling, suggesting that the Wnt/beta-catenin pathway, at least as activated by an exogenous Wnt ligand, is intact in hec mutant embryos. Our results are consistent with a role for the hecate gene in the regulation of Ca2+ release during the cleavage stages, which in turn influences dorsal gene expression in both marginal cells along the dorsoventral axis and in the enveloping layer. 相似文献