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981.
982.
983.
Comparative Ultrastructure and the Evolution of Nemertines 总被引:9,自引:1,他引:8
Nemertines are traditionally viewed as acoelomates with bloodvascular systems and a specialized cavity housing an eversibleproboscis, or, as coelomates with modified coelomic cavitiesforming the rhynchocoel, lateral vessels and gonadal sacs. Comparativeultrastructural data such as cell polarity, the occurrence ofpodocytes and mesodermal cells with rudimentary cilia for proboscis,rhynchocoel, lateral and dorsal vessels, and gonadal liningsare consistent with their interpretation as modified coelomiccavities as found, analogously, in the higher leeches. Theseand additional data regarding musculature, connective tissue,glia-like cells and gametes for 13 species affirm that nemertinesare intermediate between full-blown coelomate and acoelomategrades of organization. A comparison of ultrastructural details,however, indicates that their acoelomate attributes evolvedsecondarily from a more typical coelomate condition. 相似文献
984.
Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate 总被引:14,自引:0,他引:14
J Reid H Fung K Gehring P E Klebba H Nikaido 《The Journal of biological chemistry》1988,263(16):7753-7759
Porin, a transmembrane protein in the outer membrane of Escherichia coli, exists in a trimeric structure which is not dissociated during sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 25 degrees C. This unusual stability was utilized in the study of the conformational changes which accompany the targeting of porin to the outer membrane. A delay of 16-44 s between completion of synthesis of a monomer and its assembly into a trimer was found from the ratio of monomers to trimers found in exponentially growing cells. Pulse-chase experiments showed that rapid processing of precursor OmpF molecules was followed by assembly into sodium dodecyl sulfate-resistant oligomers with a half-time of 20 s at 30 degrees C. An intermediate in assembly was isolated by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis below 10 degrees C and was identified as a metastable dimer. 相似文献
985.
Hydrogen bonding in globular proteins 总被引:41,自引:0,他引:41
986.
Measurement of the volume change by a rapid density method upon sequential addition of calcium ion to calmodulin showed relatively large, nonuniform increases for the first 4 moles Ca2+ per mole calmodulin. Substantially larger volume increases (approximately 15 ml/mol protein) were observed upon addition of the second and fourth moles Ca2+ relative to the first and third moles added per mole calmodulin. A total volume increase of approximately 170 ml/mol protein attended the addition of 4 moles Ca2+, as expected for multidentate carboxylate coordination to metal ion. Marginal changes in volume were observed upon further additions, the data showing a remarkably sharp transition after [Ca2+]/[calmodulin] = 4. The results are consistent with an ordered binding of Ca2+ in which pair-wise additions produce similar volume changes; the volume change behavior, however, does not indicate an absence of distinct conformational states for a Ca2+(1)-calmodulin and a Ca2+(3)-calmodulin complex as has been proposed on the basis of 1H-NMR evidences. 相似文献
987.
988.
E. Schuettpelz S. B. Hoot R. Samuel F. Ehrendorfer 《Plant Systematics and Evolution》2002,231(1-4):143-151
Using two molecular data sets, the plastid atpB-rbcL intergenic spacer region and the nuclear ribosomal internal transcribed spacer regions (ITS), the taxonomic affinities of
two newly available Anemone species from the Southern Hemisphere were tested. From previous work based on morphology and geographic distribution, it
was assumed that A. tenuicaulis from New Zealand was most closely related to the Tasmanian A. crassifolia, whereas the affinity of A. antucensis from Chile and Argentina was regarded as uncertain. Analyses of molecular sequence data from these and 18 other species of
Anemone s.lat. (with Clematis as outgroup) result in trees largely congruent with past analyses based on morphology and plastid restriction site data.
They strongly support A. richardsonii and A. canadensis (with boreal distributions in the Northern Hemisphere) as paraphyletic to a well supported Southern Hemisphere clade consisting
of A. antucensis and A. tenuicaulis. This group of four species is part of an otherwise predominantly Northern Hemisphere assemblage (subgenus Anemonidium s.lat., chromosome base number x=7), including A. narcissiflora, A. obtusiloba, A. keiskeana and A. (=Hepatica) americana. All other austral species included in the present sampling, A. crassifolia (Tasmania), A. knowltonia (=Knowltonia capensis), and A. caffra (both South African), form a separate clade, sister to A. (=Pulsatilla) occidentalis and other Northern Hemisphere anemones (subgenus Anemone s.lat., x=8). Possible phytogeographical links of the Southern Hemisphere species are discussed.
Received April 23, 2001 Accepted October 4, 2001 相似文献
989.
990.
The reactions of aliphatic and aromatic amines with reducing sugars are important in both drug stability and synthesis. The
formation of glycosylamines in solution, the first step in the Maillard reaction, does not typically cause browning but results
in decreased potency and is hence significant from the aspect of drug instability. The purpose of this research was to present
(1) unreported ionic equilibria of model reactant (kynurenine), (2) the analytical methods used to characterize and measure
reaction products, (3) the kinetic scheme used to measure reaction rates and (4) relevant properties of various reducing sugars
that impact the reaction rate in solution. The methods used to identify the reversible formation of two products from the
reaction of kynurenine and monosaccharides included LC mass spectrometry, UV spectroscopy, and 1-D and 2-D 1H–1H COSY NMR spectroscopy. Kinetics was studied using a stability-indicating HPLC method. The results indicated the formation
of α and β glycosylamines by a pseudo first-order reversible reaction scheme in the pH range of 1–6. The forward reaction
was a function of initial glucose concentration but not the reverse reaction. It was concluded that the reaction kinetics
and equilibrium concentrations of the glycosylamines were pH-dependent and also a function of the acyclic content of the reacting
glucose isomer. 相似文献