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51.
52.
H M Gilles 《BMJ (Clinical research ed.)》1981,283(6303):1382-1385
53.
Michel Roberge Gilles Grondin Louise Larose Adrien R. Beaudoin 《Cell and tissue research》1981,220(4):781-786
Summary Stimulation of the exocrine pancreas by the secretagogue urecholine causes degranulation of the acinar cells. Under in vivo conditions, this degranulation is not uniform throughout the tissue. Indeed some of the acini are almost completely depleted of their granules while others display the appearance of resting acini. A noticeable feature is that all the cells of the same acinus display a comparable degree of degranulation. Moreover, groups of neighbouring acini seem to respond simultaneously suggesting that the secretory stimulus is propagated from one acinus to the other. In vitro stimulation of dispersed acini also showed that some of the acini are more responsive than others indicating that this phenomenon can not be attributed to accessibility of the secretagogue to its receptor. These observations lead us to the concept that the response of the pancreatic acinar cell is controlled at the level of the acinus. 相似文献
54.
Dr. Jacques Toupin Donald H. Marks Edwin L. Cooper Gilles Lamoureux 《In vitro cellular & developmental biology. Plant》1977,13(4):218-222
Summary Contamination and low viability of earthworm coelomocytes in tissue culture have delayed in vitro studies. Using penicillin,
streptomycin, tetracycline and Amphotericin B,Lumbricus terrestis coelomocytes were maintained viable and uncontaminated for 10 days at 15°C in medium L-15 supplemented with 5 to 10% fetal
bovine serum. The coelomocytes survived for at least 10 days with 85% viability as assessed by trypan blue exclusion assays
and phagocytosis of heat-killed yeast. Studies on the thymidine uptake, however, were negative. With the involvement of coelomocytes
in tissue graft rejection, in vitro techniques can now be applied to study their capacity in the immune response.
Supported in part by USPHS Research Grant 1 RO 1 HD09333-01 to E. L. Cooper. 相似文献
55.
The hallmark of differentiated mammary epithelial cells is a copious secretion of milk-specific components regulated by lactogenic hormones. We describe an established clonal cell line produced from primary bovine mammary alveolar cells (MAC-T) by stable transfection with SV-40 large T-antigen. MAC-T cells show a population doubling time of approximately 17 h and have been cultured more than 350 passages without showing any sign of senescence. They show the characteristic “cobblestone” morphology of epithelial cells when grown on plastic substratum. Differentiation was induced by augmenting cell-cell interaction on a floating collagen gel in the presence of prolactin. The differentiated phenotype was characterized to include (1) increased abundance in β-casein mRNA, (2) increased number and size of indirect immunofluorescent casein secretory vesicles in each cell and (3) αs- and β-casein protein secretion. The clonal nature of the cells, their immortality, and their ability to uniformly differentiate and secrete casein proteins make this cell line unique. 相似文献
56.
57.
Measurement and simulation of the morphological development of filamentous microorganisms 总被引:2,自引:0,他引:2
Growth of Streptomyces tendae was investigated in submerged culture. Images of several mycelia were analyzed by means of an image-processing system. The studies revealed that tip growth angles and branching outgrowth angles could be regarded as normally distributed. Based on these results, a random model for directional growth of hyphal tips as well as directional growth of branches is proposed. This model shows curved elongation of hyphal tips, so that the morphological development of a mycelium up to the formation of a pellet is predicted, similar to that observed in nature. 相似文献
58.
Twenty-seven mainly terpenoid constituents were identified in the essential oil of Mikania micrantha whole plant. Higher terpenoids present in the same plant included two kaurene derivatives and taraxasterol. 相似文献
59.
The sulfhydryl groups of the thiol-dependent cytolytic toxin from Bacillus alvei evidence for one essential sulfhydryl group 总被引:4,自引:0,他引:4
C Geoffroy A M Gilles J E Alouf 《Biochemical and biophysical research communications》1981,99(3):781-788
Alveolysin, an extracellular protein toxin (Mr ? 63,000) excreted by Bacillus alvei and purified to homogeneity was shown to contain four cysteine residues. All thiol groups of the hemolytically active toxin preparation were free as found by direct titration by 5,5′-dithiobis (2-nitrobenzoic acid) and confirmed by the absence of disulfide bond. Toxin alkylation with tosyl lysine chloromethyl ketone resulted in the complete loss of hemolytic activity and the disappearance of only one thiol group with no modification of histidine residues. These results support the conclusion that one essential thiol group is implicated in the membrane-disrupting activity of alveolysin. 相似文献
60.
The Neogen formations from the basin of Savigné-su-Lathan (Indre-et-Loire) have yielded 53 species of ostracods.The connections with the best known fauna from the Aquitaine basin and the Rhône basin are discussed. 相似文献