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991.
Gilbert R. D.; Schroder H.; Kawamura T.; Dale P. S.; Power G. G. 《Journal of applied physiology》1985,59(2):634-638
Heat produced by the fetus exists to the mother by one of two principal routes: by fetal-maternal exchange in the placenta or through the fetal skin to the amniotic fluid and uterine wall. We measured heat conductances along each pathway to estimate the fraction of total heat exiting each route. Thermistors were placed in the fetal aorta, two different sites in the amniotic fluid, and in a maternal artery. Five days after surgery we injected a total of 280 ml of ice-cold saline into the two separate amniotic fluid sites during a 45-s interval and measured the temperature response for the next hour. After one or two such injections the fetus was killed to cut off umbilical blood flow, and the experiment was repeated to measure the heat fluxes in the absence of placental heat exchange. Experimentally obtained temperature curves were compared with the predictions of a mathematical model. Heat conductances of the skin and uterine wall, as well as the fetal heat production, were estimated in the model using least-squares parameter optimization. In 10 fetal lambs, weighing 3.73 +/- 0.40 (SE) kg, total fetal heat production averaged 3.75 +/- 0.33 W X kg-1. The heat conductance of the uterine wall, 6.6 +/- 0.8 W X degrees C-1, was lower than that of the fetal skin, 10.2 +/- 1.0, and of the placenta, 25.7 +/- 2.9 W X degrees C-1, temperature gradient. We estimated that 84.5% of total fetal heat production exists by fetal-maternal exchange in the placenta with the remaining 15.5% exiting through the fetal skin. 相似文献
992.
In highly purified tonoplast fractions from Acer pseudoplatanus cells, the in vitro reversible phosphorylation of proteins affected only a restricted set of polypeptides. The phosphorylation process has been shown to be dramatically stimulated by calcium via the mediation of calmodulin as the transducer. The protein kinase(s) was totally inhibited by micromolar concentrations of a calmodulin antagonist. Tonoplast appears to be potentially a good experimental system for the evaluation of the effects of protein phosphorylation on membrane properties in plants.Abbreviations CaM
calmodulin
- EGTA
ethylene glycol-bis-(-amino ethylether)N,N,NN-tetraacetate
- SDS-PAGE
sodium dodecylsulphate polyacrylamide gel electrophoresis
- TCA
trichloroacetic acid 相似文献
993.
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995.
Species interactions are central to our understanding of population dynamics. While density typically strengthens competition, reducing absolute fitness, Allee effects can reverse this pattern, increasing fitness with density. Allee effects emerge in host–parasite systems when higher parasite densities dilute immune responses or increase resource‐mobilization. The optimal density of individuals in these systems should be influenced by how host quality alters the rates at which facilitative and competitive effects change across densities. We tested these ideas using sumac Rhus typhina and a gall‐forming parasite Melaphis rhois that attacks sumac leaves. Fitness peaked at intermediate densities, indicating an Allee effect, but the fitness peak depended on host sex. Patterns of abundance mirrored fitness patterns, with galls clustered on leaves and female hosts supporting greater numbers of galls. Within leaves, galls near the stem were more fit, and gall‐makers preferentially oviposited near to the stem. The patterns of fitness and abundance are consistent with Allee effects caused by increased resource mobilization at higher gall‐maker densities rather than diluted immune responses. Our results suggest that Allee effects in parasites can be described as the summative effects of competitive and facilitative processes and, because both are common, Allee effects are likely common in host–parasite systems. 相似文献
996.
Inventory of the mosquitoes (Diptera: Culicidae) of the islands of southwestern Indian Ocean, Madagascar excluded – A Critical Review. The biodiversity of mosquitoes in the islands of southwestern Indian Ocean is the concern of numerous publications. Here, we propose a synthetic inventory and the analysis of the mosquito diversity, based on the available literature. A comprehensive annotated checklist of mosquito species has been recently published on Madagascar; this is the reason why this land is excluded from our work. Studied area encompasses 28 tropical islands in the southern hemisphere: 4 islands in the Comoros archipelago, 5 Scattered Islands (îles Éparses), 5 in Mascarene, 11 in the Seychelles and 3 in the Chagos archipelago. In total, the mosquito list presents 73 valid species, of which 10 are Anophelinae and 63 Culicinae. The number of species that are distributed in these islands only is 19, i.e. 26%, which is a remarkable level for endemism. The richness in mosquito species in these islands is analysed through several aspects including geography, local speciation and natural or human dissemination. This updated inventory increases by 33% the number of known species by regard to the previous inventory published by Julvez & Mouchet in 1994. The historical responsibility of humans in the introduction of new mosquito species in these islands is strongly documented. For instance, the species with the highest distribution among islands are Aedes aegypti, Ae. albopictus and Culex quinquefasciatus. The islands belong to the afrotropical biogeographic area and, logically, the majority (63%) of mosquito species present phylogenetic affinities with continental Africa and/or Madagascar; interestingly, the number of species present in these islands and in Madagascar but absent in continental Africa is higher than the number of species present in these islands and in continental Africa but absent in Madagascar (respectively 12 and 2 species). Thanks to valuable increase in the sampling effort, our knowledge of the culicidian fauna is increasing in these islands that constitute indisputably hotspots of biodiversity. 相似文献
997.
The microsporidian pathogen Myrmecomorba nylanderiae: Intracolony transmission and impact upon tawny crazy ant (Nylanderia fulva) colonies
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The microsporidian pathogen Myrmecomorba nylanderiae Plowes et al. infects introduced tawny crazy ants (Nylanderia fulva (Mayr)), and constitutes one of the first natural enemies known to attack this invasive ant. We assess how infection is transmitted within colonies and how infection impacts N. fulva colony fragment growth under carbohydrate‐deficient and carbohydrate‐sufficient dietary conditions. Carbohydrate scarcity is a common source of stress for ant colonies. Infected workers efficiently pass infection to developing larvae but all other potential pathways for within colony transmission are rare or non‐viable. For unknown reasons, queens within infected colony fragments are generally uninfected, limiting the role of transovarial transmission in intracolony transmission. In the laboratory, infection by M. nylanderiae primarily impacts the growth of N. fulva colonies by reducing pupal production. Colony growth showed a substantially greater impact under carbohydrate‐deficient conditions implying that the effect of the pathogen may depend on seasonally variable carbohydrate availability. In the colony growth assay, worker mortality did not differ with infection status under either nutrient regime. However, in a longer, direct test of survivorship, infected worker survivorship was significantly lower. Recently, some established N. fulva populations with high prevalence of M. nylanderiae infection have declined precipitously, though it is unknown if M. nylanderiae is a causative agent in these declines. The combination of chronic impacts, presence in North America, and potential association with population declines makes M. nylanderiae a promising prospect for the biological control of N. fulva. 相似文献
998.
Eliza I. Gilbert Scott L. Durst Angela P. James Jason E. Davis Thomas B. Sinclair Nathan R. Franssen 《Environmental Biology of Fishes》2018,101(6):1067-1076
Over evolutionary time, predator-prey interactions have shaped and constrained functional and behavioral traits of piscivorous fishes. The endangered Colorado Pikeminnow Ptychocheilus lucius, a large endemic piscivore of the Colorado River Basin, encounters a substantially altered prey base that differs in behaviors and morphologies compared to the historical suite of native prey. To assess physical limitations of Colorado Pikeminnow predation, we conducted a feeding experiment with two species of nonnative prey (spined and despined Channel Catfish Ictalurus punctatus and Red Shiner Cyprinella lutrensis) and quantified scaling of cranial morphology in this predator. In our predation experiments, Colorado Pikeminnow (215–312 mm total length) consumed both spined and despined Channel Catfish as well as Red Shiner but only consumed prey less than 20% of the predator’s total length. Previous feeding trials using smaller Colorado Pikeminnow, with native and nonnative prey species, indicated they consumed prey up to 35% of their total length, suggesting relative prey size limits may decrease as this predator grows. Morphological measurements also suggested relative prey size suitability may decrease as Colorado Pikeminnow become larger, with head depth and width demonstrating isometric scaling at small sizes and shifting to negative allometry as fish get larger. Together, these data suggest an ontogenetic shift in the head morphology of Colorado Pikeminnow may decrease the relative size of prey available to these predators. In severely altered systems, understanding trophic characteristics that limit overall predator resource availability will be critical for conservation of piscivorous fishes. 相似文献
999.
Chamseddine Derabli Imen Boualia Ahmed B. Abdelwahab Raouf Boulcina Chawki Bensouici Gilbert Kirsch Abdelmadjid Debache 《Bioorganic & medicinal chemistry letters》2018,28(14):2481-2484
In this work, we describe the preparation of some new Tacrine analogues modified with a pyranopyrazole moiety. A one-pot multicomponent reaction of 3-methyl-1H-pyrazol-5(4H)-one, aryl(or hetero)aldehydes, malononitrile and cyclohexanone involving a Friedländer condensation led to the title compounds. The synthesized heterocyclic analogues of this molecule were evaluated in vitro for their AChE and BChE inhibitory activities in search for potent cholinesterase enzyme inhibitors. Most of the synthesized compounds displayed remarkable AChE inhibitory activities with IC50 values ranging from 0.044 to 5.80?µM, wherein compounds 5e and 5j were found to be most active inhibitors against AChE with IC50 values of 0.058 and 0.044?µM respectively. Molecular modeling simulation on AChE and BChE receptors, showed good correlation between IC50 values and binding interaction template of the most active inhibitors docked into the active site of their relevant enzymes. 相似文献
1000.
Ergot alkaloids are toxins produced by some species of fungi in the genus Claviceps, that may infect rye and triticale and, in a minor degree, other types of cereals. In this study, a new UHPLC-FLD method for the quantification of the six major ergot alkaloids as well as their corresponding epimers was developed. The sample preparation was done by a solid-liquid extraction with acetonitrile and clean-up via freeze-out. The method was fully validated and then applied to 39 samples (wheat, rye, triticale, and barley) harvested in Luxembourg in 2016. Samples were sieved (1.9?×?20 mm) prior to analysis in order to remove sclerotia, hosting the alkaloids. However, 23 samples still contained at least one ergot alkaloid >?LOQ and concentrations of the sum of the 6 ergot alkaloids ranged from 0.3 to 2530.1 μg/kg. Interestingly, the highest concentrations were measured in wheat and not in rye or triticale, suggesting that all kinds of cereals should be included in monitoring programs. The outcome of this study allowed giving a first overview of ergot alkaloid concentrations in cereals harvested in Luxembourg, and the measured concentrations were in similar ranges than in other parts of the world (e.g., Canada, France, Germany). 相似文献