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排序方式: 共有111条查询结果,搜索用时 15 毫秒
21.
Regional distribution of 5-methyltetrahydrofolic acid in brain 总被引:1,自引:0,他引:1
22.
Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells. 相似文献
23.
Weterings JJ Khan S van der Heden GJ Drijfhout JW Melief CJ Overkleeft HS van der Burg SH Ossendorp F van der Marel GA Filippov DV 《Bioorganic & medicinal chemistry letters》2006,16(12):3258-3261
The preparation of three different 2-alkoxy-8-hydroxyadenylpeptide conjugates has been accomplished by solid-phase synthesis combined with 'on-resin' Cu(I) catalyzed Huisgen cycloaddition. The immunogenicity of the compounds has been evaluated in IL-12 production and antigen presentation assays. 相似文献
24.
Tuin AW Siegal G van der Marel GA Overkleeft HS Filippov DV 《Bioorganic & medicinal chemistry letters》2006,16(14):3806-3808
The first synthesis of [U-13C, 15N] labeled phosphotyrosine is described. Preliminary studies toward the binding of phosphotyrosine to an SH2 domain have been performed by means of heteronuclear NMR. 相似文献
25.
Adriaan W. Tuin Gijsbert M. Grotenbreg Emile Spalburg Albert J. de Neeling Roos H. Mars-Groenendijk Gijsbert A. van der Marel Daan Noort Herman S. Overkleeft Mark Overhand 《Bioorganic & medicinal chemistry》2009,17(17):6233-6240
Loloatin C is a cyclic cationic antimicrobial peptide which is active against Gram positive as well as certain Gram negative bacteria. Unfortunately, it is equally potent against human erythrocytes. To probe the structure–activity relationship of this promising antibiotic peptide, amino acid substitution and/or incorporation of a constraint sugar amino acid dipeptide isoster has been applied. Six new derivatives have been synthesized using SPPS and their solution structure investigated using NMR studies. Finally, the antimicrobial and the hemolytic activities have been determined. 相似文献
26.
Bastiaan G. Meerburg Pieter H. Vereijken Willem de Visser Jan Verhagen Hein Korevaar Erik P. Querner Arianne T. de Blaeij Adrie van der Werf 《Wetlands Ecology and Management》2010,18(4):463-470
In Western-Europe, agricultural practices have contributed to environmental problems such as eutrophication of surface and
ground water, flooding, drought and desiccation of surrounding natural habitats. Solutions that reduce the impact of these
problems are urgently needed. Common reed (Phragmites australis) is capable of sanitizing surface water and may function as green energy source because of its high productivity. Here, the
results of an experiment in a constructed wetland in the Netherlands are presented where two different sanitation treatments
were compared. Depending on the residence time and volume per unit area, reed is capable to reduce the total amount of nitrogen
in the water with average efficiencies from 32 to 47% and the total amount of phosphorous with 27–45%. Although biomass production
still varies largely between different parts of the constructed wetland, a rapid increase in biomass was observed since planting.
Constructed wetlands with reed provide opportunities to improve water quality and reed produces enough biomass to serve as
green energy source. Moreover, these wetlands also function as a flood water reservoir and are possibly advantageous for biodiversity.
The optimal moment of reed harvesting depends on the goal of the owner. This moment should be chosen wisely, as it may have
consequences for reed filter regeneration, biomass production, biodiversity, methane emission and water sanitation efficiency. 相似文献
27.
Attemps were made to demonstrate the role of yeasts in the degradation of benzene compounds under natural soil conditions. Yeasts were isolated from acidic sandy soil supplied with benzene compounds. For this purpose the slant culture method was used. Growth on the benzene compounds took place on solid growth media at 10°C. Several yeast species were isolated: Leucosporidium scottii, Rhodotorula aurantiaca, Rhodotorula mucilaginosa, Trichosporon dulcitum, Trichosporon moniliiforme and Schizoblastosporion starkeyi-henricii. Cryptococcus humicolus and Cryptococcus laurentii were isolated from liquid enrichment cultures. All these strains assimilated several benzene compounds in pure culture.Cresol removal from contaminated soil was speeded up by inoculation with Rhodotorula aurantiaca G36. It was demonstrated that this yeast utilized this compound in competition with the soil microflora. 相似文献
28.
Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases 总被引:3,自引:0,他引:3
van Swieten PF Maehr R van den Nieuwendijk AM Kessler BM Reich M Wong CS Kalbacher H Leeuwenburgh MA Driessen C van der Marel GA Ploegh HL Overkleeft HS 《Bioorganic & medicinal chemistry letters》2004,14(12):3131-3134
Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins. 相似文献
29.
Gonçalves MA van Nierop GP Tijssen MR Lefesvre P Knaän-Shanzer S van der Velde I van Bekkum DW Valerio D de Vries AA 《Journal of virology》2005,79(5):3146-3162
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene, making it a potential target for gene therapy. There is, however, a scarcity of vectors that can accommodate the 14-kb DMD cDNA and permanently genetically correct muscle tissue in vivo or proliferating myogenic progenitors in vitro for use in autologous transplantation. Here, a dual high-capacity adenovirus-adeno-associated virus (hcAd/AAV) vector with two full-length human dystrophin-coding sequences flanked by AAV integration-enhancing elements is presented. These vectors are generated from input linear monomeric DNA molecules consisting of the Ad origin of replication and packaging signal followed by the recently identified AAV DNA integration efficiency element (p5IEE), the transgene(s) of interest, and the AAV inverted terminal repeat (ITR). After infection of producer cells with a helper Ad vector, the Ad DNA replication machinery, in concert with the AAV ITR-dependent dimerization, leads to the assembly of vector genomes with a tail-to-tail configuration that are efficiently amplified and packaged into Ad capsids. These dual hcAd/AAV hybrid vectors were used to express the dystrophin-coding sequence in rat cardiomyocytes in vitro and to restore dystrophin synthesis in the muscle tissues of mdx mice in vivo. Introduction into human cells of chimeric genomes, which contain a structure reminiscent of AAV proviral DNA, resulted in AAV Rep-dependent targeted DNA integration into the AAVS1 locus on chromosome 19. Dual hcAd/AAV hybrid vectors may thus be particularly useful to develop safe treatment modalities for diseases such as DMD that rely on efficient transfer and stable expression of large genes. 相似文献
30.
Jeroen C. Verheijen Ling Chen Suzanne F. Bayly Paul F. Torrence Gijsbert A. van der Marel J. H. Van Boom 《Nucleosides, nucleotides & nucleic acids》2013,32(10-12):1821-1830
Abstract Fully automated solid-phase synthesis gave access to a hybrid in which 5′-phosphorylated-2′-5′-linked oligoadenylate (2–5A) is connected to the 5′-terminus of DNA which, in turn, is linked at the 3′-end to PNA [2–5A-(5′)-DNA-(3′)-PNA chimera]. This novel antisense molecule retains full RNase L activation potency while suffering only a slight reduction in binding affinity. 相似文献