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Kumudini M. Weerasinghe Michael F. Scully Vijay V. Kakkar 《Biochimica et Biophysica Acta (BBA)/General Subjects》1985,839(1):57-61
Zinc chelate affinity chromatography was used to develop a rapid, three-step procedure to isolate coagulation factor XII from human plasma. The first step was ammonium sulphate fractionction which gave a 2-fold purification and 90% recovery in the 25–50% saturation fraction. The second step was zinc chelate affinity chromatography which gave a 240-fold purification and 67.5% recovery. The third step was zinc chelate affinity chromatography again, but with the application of a pH gradient. The overall recovery of zymogen factor XII was 21.7% and the total purification was 1992-fold. The purified factor XII had an apparent molecular weight of 77 600 as determined by SDS-polyacrylamide gel electrophoresis and a specific activity of 50 units/mg on a clotting assay. 相似文献
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The rumen contents of 14 sika deer (Cervus nippon) collected in November 1996 in Yamaguchi Prefecture, the western end of Honshu, Japan, were analyzed by the point-frame method. The contents were composed of high-quality foods like deciduous and evergreen browse and acorns. The composition, which consisted of a significant amount of acorns (27.8 ± 22.5%, mean ± SD), was in contrast to that of northern sika deer populations which exclusively eat graminoid leaves including dwarf bamboos. This is the first record of acorns being a major contributor to diet for wild sika deer. By the point-frame method, acorns were underestimated, and the weight contribution was much greater. 相似文献