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91.
Association of dystrobrevin and regulatory subunit of protein kinase A: a new role for dystrobrevin as a scaffold for signaling proteins 总被引:1,自引:0,他引:1
Ceccarini M Grasso M Veroni C Gambara G Artegiani B Macchia G Ramoni C Torreri P Mallozzi C Petrucci TC Macioce P 《Journal of molecular biology》2007,371(5):1174-1187
The dystrophin-related and -associated protein dystrobrevin is a component of the dystrophin-associated protein complex, which directly links the cytoskeleton to the extracellular matrix. It is now thought that this complex also serves as a dynamic scaffold for signaling proteins, and dystrobrevin may play a role in this context. Since dystrobrevin involvement in signaling pathways seems to be dependent on its interaction with other proteins, we sought new insights and performed a two-hybrid screen of a mouse brain cDNA library using beta-dystrobrevin, the isoform expressed in non-muscle tissues, as bait. Among the positive clones characterized after the screen, one encodes the regulatory subunit RIalpha of the cAMP-dependent protein kinase A (PKA). We confirmed the interaction by in vitro and in vivo association assays, and mapped the binding site of beta-dystrobrevin on RIalpha to the amino-terminal region encompassing the dimerization/docking domain of PKA regulatory subunit. We also found that the domain of interaction for RIalpha is contained in the amino-terminal region of beta-dystrobrevin. We obtained evidence that beta-dystrobrevin also interacts directly with RIIbeta, and that not only beta-dystrobrevin but also alpha-dystrobrevin interacts with PKA regulatory subunits. We show that both alpha and beta-dystrobrevin are specific phosphorylation substrates for PKA and that protein phosphatase 2A (PP2A) is associated with dystrobrevins. Our results suggest a new role for dystrobrevin as a scaffold protein that may play a role in different cellular processes involving PKA signaling. 相似文献
92.
Calandrella N Scarsella G Pescosolido N Risuleo G 《Molecular and cellular biochemistry》2007,301(1-2):155-163
Ocular hypertension is a symptom of a glaucomatous condition characterized by a severe vision decrease. Blindness caused by
the apoptotic death of the retinal ganglion cells and of the astrocytes of the optic nerve may eventually result. Experimental
hypertension was induced by inoculation of methylcellulose in the anterior chamber. Chromatin staining, TUNEL assay, and inter-nucleosomal
DNA fragmentation observed in retina and optic nerve strongly suggest that hypertension causes apoptosis. Immunolocalization
of the fibrillary acidic glial protein, specific of cell stress, and caspase-3 in the same tissues, further support this mode
of cell death. Activation of the ubiquitin dependant proteolytic system was also observed. Protection from apoptosis exerted
by administration of the peroxide scavenger trolox, suggests that the apoptotic pathway is activated by an oxidative stress.
The data presented here show that the experimental hypertensive insult induces degenerative and apoptotic events comparable
to those observed in human glaucoma. 相似文献
93.
Slc7a7 disruption causes fetal growth retardation by downregulating Igf1 in the mouse model of lysinuric protein intolerance 总被引:4,自引:0,他引:4
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95.
Demorrow S Francis H Alpini G 《Experimental biology and medicine (Maywood, N.J.)》2007,232(8):1005-1013
Biogenic amines, such as serotonin, histamine, dopamine, and the catecholamines epinephrine and norepinephrine, regulate a multitude of cellular responses. A great deal of effort has been invested into understanding the effects of these molecules and their corresponding receptor systems on cholangiocyte secretion, apoptosis, and growth. This review summarizes the results of these efforts and highlights the importance of these regulatory molecules on the physiology and pathophysiology of cholangiocytes. 相似文献
96.
The Increase in Maternal Expression of axin1 and axin2 Contribute to the Zebrafish Mutant Ichabod Ventralized Phenotype 下载免费PDF全文
Fabio Valenti Jessica Ibetti Yuko Komiya Melissa Baxter Anna Maria Lucchese Lauren Derstine Claudia Covaciu Valeria Rizzo Renza Vento Giuseppe Russo Marcella Macaluso Franco Cotelli Daniele Castiglia Cara J. Gottardi Raymond Habas Antonio Giordano Gianfranco Bellipanni 《Journal of cellular biochemistry》2015,116(3):418-430
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Pharmaceuticals are an important group of emerging pollutants with increasing interest due to their rising consumption and
the evidence for ecotoxicological effects associated to trace amounts in aquatic environments. In this paper, we assessed
the potential degradation of a series of pharmaceuticals: antibiotics (sulfamethoxazole), antidepressives (citalopram hydrobromide
and fluoxetine hydrochloride), antiepileptics (carbamazepine), anti-inflammatory drugs (diclofenac and naproxen) and estrogen
hormones (estrone, 17β-estradiol, 17α-ethinylestradiol) by means of a versatile peroxidase (VP) from the ligninolytic fungus
Bjerkandera adusta. The effects of the reaction conditions: VP activity, organic acid concentration and H2O2 addition rate, on the kinetics of the VP based oxidation system were evaluated. Diclofenac and estrogens were completely
degraded after only 5–25 min even with a very low VP activity (10 U l−1). High degradation percentages (80%) were achieved for sulfamethoxazole and naproxen. Low or undetectable removal yields
were observed for citalopram (up to 18%), fluoxetine (lower than 10%) and carbamazepine (not degraded). 相似文献
100.
Stasi LP Bhimani K Borriello M Canciani L Caselli G Colace F Ferioli C Kaswala M Mennuni L Piepoli T Pucci S Salvi M Shirsath V Zanelli T Zerbi S 《Bioorganic & medicinal chemistry letters》2011,21(21):6336-6340
The construction of a EP(4) antagonists pharmacophore model and the discovery of a highly potent oxepinic series of EP(4) antagonists is discussed. Compound 1a exhibits an excellent selectivity profile toward EP(2) receptor subtype and low cytochrome P450 inhibition potential. 相似文献