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排序方式: 共有896条查询结果,搜索用时 31 毫秒
891.
Giampaolo Manao Luigia Pazzagli Paolo Cirri Anna Caselli Guido Camici Gianni Cappugi Ahmad Saeed Giampietro Ramponi 《The protein journal》1992,11(3):333-345
Two lowM r phosphotyrosine protein phosphatases have been isolated from rat liver. The enzymes were previously known as lowM r acid phosphatases, but several recent studies have demonstrated that this family of enzymes possesses specific phosphotyrosine protein phosphatase activity. We determined the complete amino acid sequences of the two isoenzymes and named them AcP1 and AcP2. Both consist of 157 amino acid residues, are acetylated at the NH2-terminus, and have His as the COOH-terminus. The molecular weights calculated from the sequences are 18,062 for AcP1 and 17,848 for AcP2. They are homologous except in the 40–73 zone, where about 50% of residues are different. This fact suggests that the two isoenzymes are produced by an alternative splicing mechanism. There is no homology between these two isoenzymes and the receptor-like phosphotyrosine protein phosphatases LAR, CD45, human placenta PTPase 1B, and rat brain PTPase-1. AcP1 and AcP2 are also distinct from rat liver PTPase-1 and PTPase-2, since these last enzymes have higher molecular weights. AcP1 differs from AcP2 with respect to (1) substrate affinity and (2) its sensitivity to activators and inhibitors, thus suggesting a their different physiological function. 相似文献
892.
893.
Carlucci Roberto Capezzuto Francesca Cipriano Giulia D’Onghia Gianfranco Fanizza Carmelo Libralato Simone Maglietta Rosalia Maiorano Porzia Sion Letizia Tursi Angelo Ricci Pasquale 《Reviews in Fish Biology and Fisheries》2021,31(1):135-156
Reviews in Fish Biology and Fisheries - The exploitation of fishery resources acts as a driving force on cetaceans both directly, by determining their fishing mortality or injury as by-catch... 相似文献
894.
Awatef Allouch Cristina Di Primio Audrey Paoletti Gabrielle Lê-Bury Frdric Subra Valentina Quercioli Roberta Nardacci Annie David Hla Saïdi Anna Cereseto David M. Ojcius Guillaume Montagnac Florence Niedergang Gianfranco Pancino Asier Saez-Cirion Mauro Piacentini Marie-Lise Gougeon Guido Kroemer Jean-Luc Perfettini 《Cell death and differentiation》2020,27(12):3243
Understanding the viral–host cell interface during HIV-1 infection is a prerequisite for the development of innovative antiviral therapies. Here we show that the suppressor of G2 allele of skp1 (SUGT1) is a permissive factor for human immunodeficiency virus (HIV)-1 infection. Expression of SUGT1 increases in infected cells on human brain sections and in permissive host cells. We found that SUGT1 determines the permissiveness to infection of lymphocytes and macrophages by modulating the nuclear import of the viral genome. More importantly, SUGT1 stabilizes the microtubule plus-ends (+MTs) of host cells (through the modulation of microtubule acetylation and the formation of end-binding protein 1 (EB1) comets). This effect on microtubules favors HIV-1 retrograde trafficking and replication. SUGT1 depletion impairs the replication of HIV-1 patient primary isolates and mutant virus that is resistant to raltegravir antiretroviral agent. Altogether our results identify SUGT1 as a cellular factor involved in the post-entry steps of HIV-1 infection that may be targeted for new therapeutic approaches.Subject terms: Infectious diseases, Immunopathogenesis 相似文献
895.
Gianfranco Adimari Andrea Sinigaglia 《Biometrical journal. Biometrische Zeitschrift》2020,62(6):1463-1475
In medical research, diagnostic tests with continuous values are widely employed to attempt to distinguish between diseased and non-diseased subjects. The diagnostic accuracy of a test (or a biomarker) can be assessed by using the receiver operating characteristic (ROC) curve of the test. To summarize the ROC curve and primarily to determine an “optimal” threshold for test results to use in practice, several approaches may be considered, such as those based on the Youden index, on the so-called close-to-(0,1) point, on the concordance probability and on the symmetry point. In this paper, we focus on the symmetry point-based approach, that simultaneously controls the probabilities of the two types of correct classifications (healthy as healthy and diseased as diseased), and show how to get joint nonparametric confidence regions for the corresponding optimal cutpoint and the associated sensitivity (= specificity) value. Extensive simulation experiments are conducted to evaluate the finite sample performances of the proposed method. Real datasets are also used to illustrate its application. 相似文献
896.