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51.
Natale Cascinelli Gian Paolo Balzarini Vincenzo Fontana Sergio Orefice Umberto Veronesi 《Cancer immunology, immunotherapy : CII》1977,2(3):157-161
Summary From November 1973 to December 1974, 20 patients with advanced malignant melanoma were treated with BCG given by intralymphatic route at the Cancer Institute of Milan. The lyophilized Pasteur BCG was used. Patients were treated with a single dose ranging from 0.2–80 mg. Patients' performance status was never severely impaired.The most frequent side effects were fever, lymphangitis, and lymph node enlargement.Variations were observed in white cell count, ERS and immunoglobulins; in no case did we find evidence of liver toxicity or tumor growth enhancement. It is concluded that the intralymphatic route is a safe way of administrating BCG. 相似文献
52.
Roberta Bertelli Fabrizio Ginevri Rosanna Gusmano Gian Marco Ghiggeri 《In vitro cellular & developmental biology. Animal》1991,27(10):799-804
Summary It has been suggested that the generation of toxic radicals plays an important role in toxicity by Adriamycin (ADR) on cancer
cell lines and in vivo. We have examined the role of free radicals in determining toxicity and resistance to ADR of rat glomerular
epithelial cells in culture; this method provides a good model for analyzing the mechanisms responsible for ADR experimental
nephrosis in rats. Three points were established: a) the intra- or extracellular site of ADR toxicity; b) the role of the
superoxide anion and of the hydroxyl radical in determining intra- and-extracellular cytotoxicity; and c) the implication
of oxido-reduction cycling as a potential route for ADR semiquinone transformation. Free ADR was found to induce the same
inhibition of [3H]thymidine incorporation into DNA as ADR bound to an agarose macroporous bed which prevents the intracellular incorporation
of the drug. Specific scavenging of free radical activity by the enzymes catalase and superoxide dismutase, the hydroxyl radical
inhibitors dimethyl sulfoxide and dimethylthiourea (DMTU) and by chelation of intracellular free iron with deferoxamine produced
only a partial restoration of [3H]thymidine incorporation into DNA, which was maximal for DMTU (30% of normal incorporation). DMTU treatment was unsuccessful
in preventing the extracellular cytostatic effect of ADR. Finally, glomerular epithelial cell killing (51Cr-release method) by 5-iminodaunorubicin, an ADR analogue with a modified quinone function that prohibits oxido-reduction
cycling, was higher than unmodified ADR. These results indicate that ADR may exert its cytotoxic effects on glomerular epithelial
cells by interaction at the cell surface, whereas the intracellular compartment, principally DNA, does not seem to be the
target of ADR effects. They also suggest that the free radicals are in part responsible for ADR intracellular cytotoxicity,
but other mechanisms should also be hypothesized. Finally, the participation of the ADR semiquinone radical in oxido-reduction
cycling seems not important for the induction of the cellular damage. 相似文献
53.
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55.
Bortolotti U Casarotto D Frugoni C De Mozzi P Thiene G Gallucci V 《Cardiovascular diseases》1981,8(2):250-258
Over a 2-year period, 19 patients whose autologous saphenous veins were either unsuitable or unavailable underwent myocardial revascularization with saphenous vein allografts (SVAs) at our institution. All SVAs had been preserved in 98% glycerol at room temperature for at least 3 weeks (average, 7 weeks); before use, they were rinsed with saline and antibiotic solution. One operative death (5.2%) and three late deaths (16.6%) occurred. Fourteen of the long-term survivors have been observed for 24 to 48 months (average 32.2 months) postoperatively. Nine are asymptomatic, whereas four complain of angina, and one reports exertional dyspnea with-out chest pain. Only three patients have been restudied (7, 10, and 18 months after surgery, respectively); in each of these patients, angiography has shown occlusion of all SVAs. However, histologic examination of glycerol-preserved SVAs has not revealed pathologic changes that would suggest potential graft failure. Despite fairly satisfactory clinical results, preliminary hemodynamic data indicate that glycerol-preserved SVAs are unsuitable for myocardial revascularization in the absence of autologous saphenous veins. 相似文献
56.
Vincenzo Facchini John A. Timbrell 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1980,183(2):167-173
Methods are described for the determination of 4-N-acetylhydrazinophthalazin-1-one, 4-hydrazinophthalazin-1-one, phthalazinone and s-triazolo[3,4-a]phthalazine in human urine.4-Hydrazinophthalazin-1-one and 4-N-acetylhydrazinophthalazin-1-one (following acid hydrolysis) are reacted with acetylacetone to give a distinctive pyrazole derivative which can be determined by gas chromatography using a nitrogen-specific detector.Phthalazinone and s-triazolo[3,4-a]phthalazine are measured underivatised by high-performance liquid chromatography. 相似文献
57.
Vincenzo Panagia Clayton E. Heyliger Patrick C. Choy Naranjan S. Dhalla 《生物化学与生物物理学报:生物膜》1981,640(3)
The influence of a phosphatidylinositol-specific phospholipase C treatment on rat heart sarcolemmal 5′-nucleotidase was investigated. Upon complete hydrolysis of all phosphatidylinositol in the sarcolemma, 75% of 5′-nucleotidase activity was found in the solubilized form. The insolubilized enzyme after this treatment has the same Km for AMP as the untreated, sarcolemmal-bound enzyme (0.04 mM), whereas the solubilized enzyme has a 40-fold increase in Km for AMP (0.16 mM). Other sarcolemmal-bound enzymes were not affected by the same treatment. Hence, the specific involvement of phosphatidylinositol in the binding of 5′-nucleotidase to the sarcolemma of the rat heart is clearly demonstrated. 相似文献
58.
Peter Skrabal Vincenzo Rizzo Antonio Baici Felix Bangerter Pier Luigi Luisi 《Biopolymers》1979,18(4):995-1008
The 1H-nmr spectra of co-oligopeptides of tryptophan and glycine with structure H-Gly-Trp-(Gly)n-Trp-Gly-OH (n = 0–2) and those of several di- and tripeptides have been recorded at 360 MHz with CD3OD solutions containing 0.1N NaOD. The assignment of resonance signals was generally possible by comparing the spectra of structurally related peptides with each other. In order to solve the remaining ambiguities in the assignment, H-(αL,βS)(α,β-d2)Trp-OH, H-Trp-(αL,βS)(α,β-d2)Trp-OH, and H-Trp-(δ1,ε2,ζ2,ζ3,η2-d5)Trp-OH have been prepared and their spectra compared with those of the undeuterated compounds. The distribution of rotamers around the χ1 and (in two cases) χ2 torsion angles of the side chains has been obtained from the vicinal coupling constants 3J and from the long-range coupling constants 4J. These data and an analysis of the chemical shifts of the Gly-Cα protons suggest that the orientation of the aromatic side chain is influenced by the following order of decreasing interaction with the functional groups at N- and C-side: -NH2 > –NHCO– > –CONH–> –COO?. This rule does not hold for the second Trp residue of di- and tripeptides containing the -Trp-Trp- sequence, which has tentatively been attributed to steric effects. 相似文献
59.
Summary A model of a thoracolumbar somite of a chick embryo at the 53rd incubation hour was obtained by mathematical methods, after identification of somite cell types by means of electron microscopy.Each specific district occupied by the cell types was precisely determined.On the basis of these observations, the somite was three-dimensionally reconstructed and the spatial positions of the primitive myotome, dermatome, sclerotome, undifferentiated mesoderm and myocele were precisely identified. 相似文献
60.
Derek Jones Giuseppe Disteffano Claudio Toniolo Gian Maria Bonora 《Biopolymers》1978,17(11):2703-2713
The problem of the quantitative determination of sulfoxide groups in peptide molecules has been re-examined. The approaches currently available for the estimation of δ-sulfoxide amino acids are limited in number and characterized by serious shortcomings; in addition, the choice of methods for the estimation of γ-sulfoxide amino acids is even more restricted. A new, rapid, and nondestructive direct method for determining quantitatively all types of sulfoxides in peptide molecules by using x-ray photoelectron spectroscopy is described. 相似文献