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101.
102.
Mitochondrial permeability transition involves dissociation of F1FO ATP synthase dimers and C‐ring conformation 下载免费PDF全文
Massimo Bonora Claudia Morganti Giampaolo Morciano Gaia Pedriali Magdalena Lebiedzinska‐Arciszewska Giorgio Aquila Carlotta Giorgi Paola Rizzo Gianluca Campo Roberto Ferrari Guido Kroemer Mariusz R Wieckowski Lorenzo Galluzzi Paolo Pinton 《EMBO reports》2017,18(7):1077-1089
The impact of the mitochondrial permeability transition (MPT) on cellular physiology is well characterized. In contrast, the composition and mode of action of the permeability transition pore complex (PTPC), the supramolecular entity that initiates MPT, remain to be elucidated. Specifically, the precise contribution of the mitochondrial F1FO ATP synthase (or subunits thereof) to MPT is a matter of debate. We demonstrate that F1FO ATP synthase dimers dissociate as the PTPC opens upon MPT induction. Stabilizing F1FO ATP synthase dimers by genetic approaches inhibits PTPC opening and MPT. Specific mutations in the F1FO ATP synthase c subunit that alter C‐ring conformation sensitize cells to MPT induction, which can be reverted by stabilizing F1FO ATP synthase dimers. Destabilizing F1FO ATP synthase dimers fails to trigger PTPC opening in the presence of mutants of the c subunit that inhibit MPT. The current study does not provide direct evidence that the C‐ring is the long‐sought pore‐forming subunit of the PTPC, but reveals that PTPC opening requires the dissociation of F1FO ATP synthase dimers and involves the C‐ring. 相似文献
103.
M Stefani A Berti G Camici G Manao G Cappugi G Ramponi 《Physiological chemistry and physics》1978,10(4):367-373
Horse muscle acylphosphatase consists of a main chain S-S bound to glutathione. It was found that removal of the glutathione by reduction and successive carboxymethylation of the only cysteine of the main chain affects the stability of the enzyme, mainly with respect to thermal inactivation. On the other hand, the kinetic properties of the enzyme are affected very little. 相似文献
104.
Zooplankton variability in a lagoon of the Po Delta, the Sacca di Goro, was studied in relation to changes in its hydrodynamic and hydrological features. From 1987 to 1992 the lagoon was affected each summer by severe anoxia due to the decomposition of dense and widespread beds of Ulva rigida. In August 1992 a canal was opened through the sand bank closing off the lagoon from the sea in order to improve the water circulation. This hydraulic intervention led to significant environmental changes in the lagoon: during the two subsequent years the summer dystrophic crises were brief and less severe, due to a sharp decrease in the Ulva cover. At the same time a clear increase in phytoplankton biomass values was observed. Following the opening of the channel, the most remarkable change in zooplankton was a significant density increase of calanoids, particularly of Acartia tonsa. 相似文献
105.
The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme. 相似文献
106.
The mitochondrial permeability transition pore: an evolving concept critical for cell life and death
Giampaolo Morciano Natalia Naumova Piotr Koprowski Sara Valente Vilma A. Sardão Yaiza Potes Alessandro Rimessi Mariusz R. Wieckowski Paulo J. Oliveira 《Biological reviews of the Cambridge Philosophical Society》2021,96(6):2489-2521
In this review, we summarize current knowledge of perhaps one of the most intriguing phenomena in cell biology: the mitochondrial permeability transition pore (mPTP). This phenomenon, which was initially observed as a sudden loss of inner mitochondrial membrane impermeability caused by excessive calcium, has been studied for almost 50 years, and still no definitive answer has been provided regarding its mechanisms. From its initial consideration as an in vitro artifact to the current notion that the mPTP is a phenomenon with physiological and pathological implications, a long road has been travelled. We here summarize the role of mitochondria in cytosolic calcium control and the evolving concepts regarding the mitochondrial permeability transition (mPT) and the mPTP. We show how the evolving mPTP models and mechanisms, which involve many proposed mitochondrial protein components, have arisen from methodological advances and more complex biological models. We describe how scientific progress and methodological advances have allowed milestone discoveries on mPTP regulation and composition and its recognition as a valid target for drug development and a critical component of mitochondrial biology. 相似文献
107.
Kazuki Harada Manao Ozawa Kanako Ishihara Ryoji Koike Tetsuo Asai Hitoshi Ishikawa 《Microbiology and immunology》2009,53(2):107-111
Penner serotypes of C. jejuni in a total of 601 isolates from apparently healthy cattle, layer and broiler chickens in Japan were examined between 2001 and 2006. Predominant serotypes were B (O: 2, 19.1%), D (O: 4, 13.5%), Y (O: 37, 7.3%) and G (O: 8, 5.8%), whereas the remaining serotypes made up less than 5% of the total isolates. The frequency of ampicillin resistance in serotype G (65.6%) was significantly higher than in serotypes D (12.5%), B (11.2%), and Y (0%). Our results suggest that serotype is one factor contributing to the prevalence of ampicillin resistance in C. jejuni isolates. 相似文献
108.
Vena P Franzoso G Gastaldi D Contro R Dallolio V 《Computer methods in biomechanics and biomedical engineering》2005,8(1):7-16
The biomechanical compatibility of an interspinous device, used for the "dynamic stabilization" of a diseased spinal motion segment, was investigated. The behaviour of an implant made of titanium based alloy (Ti6Al4V) and that of an implant made of a super-elastic alloy (Ni-Ti) have been compared. The assessment of the biomechanical compatibility was achieved by means of the finite element method, in which suitable constitutive laws have been adopted for the annulus fibrosus and for the metal alloys. The model was aimed at simulating the healthy, the nucleotomized and the treated L4-L5 lumbar segment, subjected to compressive force and flexion-extension as well as lateral flexion moments. The computational model has shown that both the implants were able to achieve their main design purpose, which is to diminish the forces acting on the apophyseal joints. Nevertheless, the Ni-Ti implant has shown a more physiological flexural stiffness with respect to the Ti6Al4V implant, which exhibited an excessive stiffness and permanent strains (plastic strains), even under physiological loads. The computational models presented in this paper seems to be a promising tool able to predict the effectiveness of a biomedical device and to select the materials to be used for the implant manufacturing, within an engineering approach to the clinical problem of the spinal diseases. 相似文献
109.
Biological relevance of volatile organic compounds emitted during the pathogenic interactions between apple plants and Erwinia amylovora 下载免费PDF全文
110.
Limnological research on northern Apennine lakes (Italy) in relation to eutrophication and acidification risk 总被引:1,自引:0,他引:1
Pierluigi Viaroli Ireneo Ferrari Gianmarco Paris Giampaolo Rossetti Paolo Menozzi 《Hydrobiologia》1994,274(1-3):155-162
Substantial variability was found in the water chemistry of 22 northern Apennine lakes. In a group of lakes there is evidence
of disturbance linked to eutrophication processes. Other lakes showed weak ion concentrations and alkalinity below the acidification
risk threshold. However no acidified lakes were found. The lack of waterbodies with severely altered hydrochemistry may explain
why no clear relationship between plankton community structure and water chemistry was observed. 相似文献