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Multivariate data analysis in biochemistry: a new integrative approach to metabolic control in brain aging. 总被引:1,自引:0,他引:1
A Giuliani G Capuani A Miccheli T Aureli M T Ramacc F Conti 《Cellular and molecular biology, including cyto-enzymology》1991,37(6):631-638
The principal component analysis (PCA) allows to obtain a quantitative measure of the state of metabolism as a whole. In this paper we applied this method to the study of energy metabolism during aging process and of the effect of a drug (Acetyl-1-carnitine, ALCAR) on the aging brain. 相似文献
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Tooth grooves,occlusal striations,dental calculus,and evidence for fiber processing in an Italian eneolithic/bronze age cemetery 下载免费PDF全文
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Shun Lu Maximiliano Giuliani Hanjeong Harvey Lori?L. Burrows Robert?A. Wickham John?R. Dutcher 《Biophysical journal》2015,108(12):2865-2875
Type IV pili (T4P) are very thin protein filaments that extend from and retract into bacterial cells, allowing them to interact with and colonize a broad array of chemically diverse surfaces. The physical aspects that allow T4P to mediate adherence to many different surfaces remain unclear. Atomic force microscopy (AFM) nanoscale pulling experiments were used to measure the mechanical properties of T4P of a mutant strain of Pseudomonas aeruginosa PAO1 unable to retract its T4P. After adhering bacteria to the end of an AFM cantilever and approaching surfaces of mica, gold, or polystyrene, we observed adhesion of the T4P to all of the surfaces. Pulling of single and multiple T4P on retraction of the cantilever from the surfaces could be described using the worm-like chain (WLC) model. Distinct peaks in the measured distributions of the best-fit values of the persistence length Lp on two different surfaces provide strong evidence for close-packed bundling of very flexible T4P. In addition, we observed force plateaus indicating that adhesion of the T4P to both hydrophilic and hydrophobic surfaces occurs along extended lengths of the T4P. These data shed new light, to our knowledge, on T4P flexibility and support a low-affinity, high-avidity adhesion mechanism that mediates bacteria-surface interactions. 相似文献
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Synthesis of pentylferulate by a feruloyl esterase from Aspergillus niger using water-in-oil microemulsions 总被引:2,自引:0,他引:2
S. Giuliani C. Piana L. Setti A. Hochkoeppler P.G. Pifferi G. Williamson C.B. Faulds 《Biotechnology letters》2001,23(4):325-330
Pentylferulate synthesis was achieved at high yields (50–60%) with Aspergillus niger feruloyl esterase using a water-in-oil microemulsion system. The initial rate of synthesis decreased by 15–20% when the water content of the microemulsion was increased from 1.8 to 2.4% (v/v), although a concomitant decrease in conversion was not observed. The enzyme stability was significantly higher in the microemulsion than in an aqueous solution. 相似文献
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