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191.
192.
Chia-Jung Chang Ho-Chang Kuo Jeng-Sheng Chang Jong-Keuk Lee Fuu-Jen Tsai Chiea Chuen Khor Li-Ching Chang Shih-Ping Chen Tai-Ming Ko Yi-Min Liu Ying-Ju Chen Young Mi Hong Gi Young Jang Martin L. Hibberd Taco Kuijpers David Burgner Michael Levin Jane C. Burns Sonia Davila International Kawasaki Disease Genetics Consortium? Korean Kawasaki Disease Genetics Consortium? Taiwan Kawasaki Disease Genetics Consortium? Yuan-Tsong Chen Chien-Hsiun Chen Jer-Yuarn Wu Yi-Ching Lee 《PloS one》2013,8(8)
The BLK and CD40 loci have been associated with Kawasaki disease (KD) in two genome-wide association studies (GWAS) conducted in a Taiwanese population of Han Chinese ancestry (Taiwanese) and in Japanese cohorts. Here we build on these findings with replication studies of the BLK and CD40 loci in populations of Korean and European descent. The BLK region was significantly associated with KD susceptibility in both populations. Within the BLK gene the rs2736340-located linkage disequilibrium (LD ) comprising the promoter and first intron was strongly associated with KD, with the combined results of Asian studies including Taiwanese, Japanese, and Korean populations (2,539 KD patients and 7,021 controls) providing very compelling evidence of association (rs2736340, OR = 1.498, 1.354–1.657; P = 4.74×10−31). We determined the percentage of B cells present in the peripheral blood mononuclear cell (PBMC) population and the expression of BLK in the peripheral blood leukocytes (leukocytes) of KD patients during the acute and convalescent stages. The percentage of B cells in the PBMC population and the expression of BLK in leukocytes were induced in patients in the acute stage of KD. In B cell lines derived from KD patients, and in purified B cells from KD patients obtained during the acute stage, those with the risk allele of rs2736340 expressed significantly lower levels of BLK. These results suggest that peripheral B cells play a pathogenic role during the acute stage of KD. Decreased BLK expression in peripheral blood B cells may alter B cell function and predispose individuals to KD. These associative data suggest a role for B cells during acute KD. Understanding the functional implications may facilitate the development of B cell-mediated therapy for KD. 相似文献
193.
194.
Mi Yoon Chung Jordi López-Pujol Myung-Ok Moon Jae Min Chung Chan Soo Kim Byung-Yun Sun Ki-Joong Kim Myong Gi Chung 《Population Ecology》2013,55(1):159-172
We compared allozyme variation in the two arctic–alpine plants Diapensia lapponica var. obovata and Empetrum nigrum var. japonicum between Sakhalin Island in Russian Far East, within their range core, and the Korean island of Jeju, their world’s southernmost distribution. For D. lapponica var. obovata, Sakhalin populations harbored moderate levels of within-population genetic variation and low among-population divergence, whereas extremely low levels of within-population genetic diversity and high among-population differentiation were found in Jeju Island populations. In contrast, we found moderate levels of within-population variation and low among-population differentiation in E. nigrum var. japonicum in both northern populations (those of Sakhalin and an additional population from northern Japan) and Jeju Island populations. Under a similar scenario of immigration history of arctic–alpine plants on Jeju Island during the glacial periods of the Pleistocene and local persistence through glacial/interglacial cycles, the contrasting genetic structure between D. lapponica var. obovata and E. nigrum var. japonicum is mainly attributable to their different life-history, ecological, and demographic traits: (1) hermaphroditic versus monoecious, dioecious or polygamous, (2) seeds with no adaptations for long-distance dispersal versus berry-like drupes dispersed by animals and birds, and (3) a very small patch near the peak of Mt. Halla with a few hundred individuals versus a relatively continuous distribution around the peak of Mt. Halla with numerous individuals. From a conservation perspective, in situ and ex situ conservation measures should be strengthened for D. lapponica var. obovata on Jeju Island given their extreme rarity there. 相似文献
195.
Jae Il Yoo Jung Wook Kim Gi Su Kang Hwa Su Kim Jung Sik Yoo Yeong Seon Lee 《Journal of microbiology (Seoul, Korea)》2013,51(2):160-165
Vancomycin intermediate Staphylococcus aureus (VISA) strains are increasingly prevalent in the hospital setting, and are of major concern in the treatment of methicillin-resistant S. aureus infections. Multiple mutations in vancomycin-susceptible S. aureus (VSSA) strains likely led to the emergence of VISA, and point mutations in the agr, orf1, yvqF, vraSR, graSR, and tcaRAB genes of VISA strains have been shown to contribute to glycopeptide resistance. Therefore, we investigated point mutations in these genes from 87 VISA and 27 VSSA clinical strains isolated from Korean hospitals. All strains were assigned an agr type (I, II, or III) on the basis of multiplex PCR, with the majority of VISA strains belonging to agr groups I and II. Sequencing revealed amino acid changes in vraS from VISA strains which were not present in the VSSA strains. The E59D substitution in the vraR gene occurred in 36.3% of VSSA/agrI and 92.7% of VISA/agrI strains, suggesting that this mutation associated with emergence of VISA/agrI strains. VISA strains were classified into 31 mutation patterns according to mutations in the yvqF, vraSR, graSR, and tcaRAB genes. In addition, the mutation patterns were correlated with agr and sequence type (ST). The most prevalent pattern included agr type I (ST 72) strains with E59D (vraR), L26F and T224I (graS), D148Q (graR), and L218P, R283H and G312D (tcaA) amino acid substitutions. The minimum inhibitory concentration (MIC) range of mutation pattern 5 toward oxacillin and imipenem was much lower than that of patterns 6 and 24. These results improve our understanding of emergence of VISA strains. 相似文献
196.
Two marine urostylid ciliates, Anteholosticha multicirrata n. sp. and Anteholosticha pulchra (Kahl, 1932) Berger, 2003, were collected from South Korea. These species were identified based on morphology, morphogenesis, and SSU rRNA gene sequence comparison. Anteholosticha multicirrata n. sp. is characterized by the following features: body size 90–125 × 30–45 μm in vivo, shape slender to ellipsoidal in outline, with yellow‐greenish cortical granules distributed along and between dorsal kineties and cirri; single contractile vacuole positioned on left at mid‐body; three frontal, five to seven frontoterminal, one buccal, one to two pretransverse and four to six transverse cirri; three complete dorsal kineties; one left and one right marginal cirral row; about 117 macronuclear nodules; and three to four micronuclei observed during morphogenesis. In addition, based on the observations of morphogenesis, we found that A. pulchra has pretransverse cirri, which were not described in detail in previous studies. Nuclear small subunit ribosomal RNA (SSU rRNA) gene was used to analyse their phylogenetic relationship, and the gene tree supports that the genus Anteholosticha is a highly polyphyletic group. 相似文献
197.
Park SH Kim JJ Chung JS Lee SR Lee GY Kim HJ Yoo YD 《Biochemical and biophysical research communications》2011,(1):149-153
Dendritic cells are the most potent antigen presenting cells responsible for the development of immune responses in cancer. However, it is known that the function of dendritic cells in tumor-bearing hosts is severely compromised. Our previous studies demonstrated that the defects in dendritic cell function are due, to a large extent, to the accumulation of high amounts of lipids – predominantly triglycerides – in a substantial proportion of dendritic cells in tumor-bearing mice and patients with cancer. The dendritic cells accumulation of lipids is likely associated with their up-regulation of a scavenger receptor A. This receptor is primarily responsible for uptake of modified lipids. Here, by using different versions of liquid chromatography–mass spectrometry, we identified several molecular species of oxygenated lipids in plasma of tumor-bearing animals that may be responsible for their uptake and accumulation by dendritic cells via scavenger receptor A-dependent pathway – the effect that may be associated with the loss of dendritic cell’s immune surveillance function in cancer. 相似文献
198.
As a special reference of the nuclear cistron rDNA of cyclopoid copepods, we obtained the entire DNA sequence of a single
rDNA repeat unit from Paracyclopina nana (Cyclopoida: Cyclopettidae). The genomic organization of P. nana rDNA (7,974 bp, 51.7% of GC) was observed to be 18S–ITS1–5.8S–ITS2–28S–IGS in order. Comparative analyses of the rDNA between
P. nana and other copepods showed that the intergenic spacer (IGS) was highly informative and divergent, while other coding regions
are relatively conserved. We detected eleven poly(T) tracts in the IGS, demonstrating the high AT content in this region.
In addition, many sub-repeat sequence patterns (e.g., AG, AT, GC, CCG, TC) such as microsatellites were identified from the
rDNA IGS of P. nana. In this article, we show the first complete sequence of rDNA from the order Cyclopoida, providing a better understanding
of molecular characteristics in molecular taxonomy. 相似文献
199.
Kim MJ Shin KS Jeon JH Lee DR Shim SH Kim JK Cha DH Yoon TK Kim GJ 《Cell and tissue research》2011,346(1):53-64
Placenta-derived stem cells (PDSCs) have gained interest as an alternative source of stem cells for regenerative medicine
because of their potential for self-renewal and differentiation and their immunomodulatory properties. Although many studies
have characterized various PDSCs biologically, the properties of the self-renewal and differentiation potential among PDSCs
have not yet been directly compared. We consider the characterization of chorionic-plate-derived mesenchymal stem cells (CP-MSCs)
and Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) among various PDSCs and the assessment of their differentiation
potential to be important for future studies into the applicability and effectiveness of PDSCs in cell therapy. In the present
study, the capacities for self-renewal and multipotent differentiation of CP-MSCs and WJ-MSC isolated from normal term placentas
were compared. CP-MSCs and WJ-MSCs expressed mRNAs for the pluripotent stem cell markers Oct-4, Nanog, and Sox-2. Additionally,
HLA-G for immunomodulatory effects was found to be expressed at both the mRNA and protein levels in both cell types. The CP-MSCs
and WJ-MSCs also had the capacities to differentiate into cells of mesodermal (adipogenic and osteogenic) and endodermal (hepatogenic)
lineages. Expression of adipogenesis-related genes was higher in CP-MSCs than in WJ-MSCs, whereas WJ-MSCs accumulated more
mineralized matrix than CP-MSCs. The WJ-MSCs expressed more of CYP3A4 mRNA, a marker for mature hepatocytes, than CP-MSCs.
Thus, we propose that CP-MSCs and WJ-MSCs are useful sources of cells for appropriate clinical applications in the treatment
of various degenerative diseases. 相似文献
200.
Kim JJ Hong YM Sohn S Jang GY Ha KS Yun SW Han MK Lee KY Song MS Lee HD Kim DS Lee JE Shin ES Jang JH Lee YS Kim SY Lee JY Han BG Wu JY Kim KJ Park YM Seo EJ Park IS Lee JK;Korean Kawasaki Disease Genetics Consortium 《Human genetics》2011,129(5):487-495
Kawasaki disease (KD) is an acute self-limited vasculitis of infants and children that manifests as fever and signs of mucocutaneous inflammation. Coronary artery aneurysms develop in approximately 15-25% of untreated children. Although the etiology of KD is largely unknown, epidemiologic data suggest the importance of genetic factors in the susceptibility to KD. In order to identify genetic variants that influence KD susceptibility, we performed a genome-wide association study (GWAS) using Affymetrix SNP array 6.0 in 186 Korean KD patients and 600 healthy controls; 18 and 26 genomic regions with one or more sequence variants were associated with KD and KD with coronary artery lesions (CALs), respectively (p?1?×?10(-5)). Of these, one locus on chromosome 1p31 (rs527409) was replicated in 266 children with KD and 600 normal controls (odds ratio [OR]?=?2.90, 95% confidence interval [CI]?=?1.85-4.54, P (combined)?=?1.46?×?10(-6)); and a PELI1 locus on chromosome 2p13.3 (rs7604693) was replicated in 86 KD patients with CALs and 600 controls (OR?=?2.70, 95% CI?=?1.77-4.12, P (combined)?=?2.00?×?10(-6)). These results implicate a locus in the 1p31 region and the PELI1 gene locus in the 2p13.3 region as susceptibility loci for KD and CALs, respectively. 相似文献