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11.
Chattopadhyay MB Mukherjee S Kulkarni I Vijayan V Doloi M Kanjilal N Chatterjee M 《Cancer cell international》2005,5(1):16
Combined effect of vanadium and beta-carotene on rat liver DNA-chain break and Proton induced X-ray emission (PIXE) analysis
was studied during a necrogenic dose (200 mg/kg of body weight) of Diethyl Nitrosamine (DENA) induced rat liver carcinogenesis.
Morphological and histopathological changes were observed as an end point biomarker. Supplementation of vanadium (0.5 ppm
ad libitum) in drinking water and beta-carotene in the basal diet (120 mg/Kg of body weight) were performed four weeks before DENA treatment
and continued till the end of the experiment (16 weeks). PIXE analysis revealed the restoration of near normal value of zinc,
copper, and iron, which were substantially altered when compared to carcinogen treated groups. Supplementation of both vanadium
and beta-carotene four weeks before DENA injection was found to offer significant (64.73%, P < 0.001) protection against generation
of single-strand breaks when compared with the carcinogen control counter parts. A significant stabilization of hepatic architecture
of the cells was observed as compared to carcinogen control in vanadium plus beta-carotene treated group. This study thus
suggests that vanadium, a prooxidant but potential therapeutic agent yield safe and effective pharmacological formulation
with beta-carotene, an antioxidant, in the inhibition of experimental rat hepatocarcinogenesis. 相似文献
12.
Hunter NB Moseley 《BMC bioinformatics》2010,11(1):139
Background
Stable isotope tracing with ultra-high resolution Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) can provide simultaneous determination of hundreds to thousands of metabolite isotopologue species without the need for chromatographic separation. Therefore, this experimental metabolomics methodology may allow the tracing of metabolic pathways starting from stable-isotope-enriched precursors, which can improve our mechanistic understanding of cellular metabolism. However, contributions to the observed intensities arising from the stable isotope's natural abundance must be subtracted (deisotoped) from the raw isotopologue peaks before interpretation. Previously posed deisotoping problems are sidestepped due to the isotopic resolution and identification of individual isotopologue peaks. This peak resolution and identification come from the very high mass resolution and accuracy of FT-ICR-MS and present an analytically solvable deisotoping problem, even in the context of stable-isotope enrichment. 相似文献13.
Charlie T Cheng Enrique M Ostrea Jr Joseph NB Alviedo Felix P Banadera Ronald L Thomas 《Experimental biology and medicine (Maywood, N.J.)》2021,246(4):380
To determine if meconium fatty acid ethyl esters (FAEE) in rat pups is a good biomarker of prenatal exposure and effect to alcohol, three groups of pregnant rats were studied: one control (pair fed) and two treatment groups given 25% alcohol at 2.2 or 5.5 g−1 kg−1 d−1. The pups were delivered on day 20 and, for each dam, were separated into a male and female group. The body, brain, intestines, and placenta of the pups were obtained, weighed, and stored at −20°C. The pups’ intestines (as surrogate of meconium) from each group were pooled, and meconium was analyzed by gas chromatography/mass spectroscopy for FAEE. The meconium showed the following FAEE: ethyl palmitate, ethyl stearate, and ethyl linolenate and were only found in the alcohol-treated group and with high specificity but low sensitivity. Mean body weight of the pups was lower in the treatment groups compared to the control groups. Ethyl palmitate concentration correlated negatively to the pups’ mean body and brain weights. Therefore, ethyl palmitate, stearate, and linolenate, in meconium of rat pups prenatally exposed to alcohol, are useful biomarkers of prenatal alcohol exposure, with ethyl palmitate a good biomarker of adverse effect on the pups’ body and brain weight. 相似文献