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61.
Shahabzadeh Zeinab Darvishzadeh Reza Mohammadi Reza Jafari Morad 《Plant Molecular Biology Reporter》2020,38(2):187-188
Plant Molecular Biology Reporter - The original version of this article unfortunately contained some mistakes in article Title and Table 1. 相似文献
62.
Reza Jafarzadeh-Esfehani Seyed Mostafa Parizadeh Amirsaeed Sabeti Aghabozorgi Negar Yavari Ariane Sadr-Nabavi Seyed Alireza Parizadeh Maryam Ghandehari Afsane Javanbakht Afsaneh Rezaei-Kalat Seyed Mahdi Hassanian Mohammad Vojdanparast Gordon A. Ferns Majid Khazaei Amir Avan 《Journal of cellular physiology》2020,235(10):6393-6403
Venous and arterial thrombosis are conditions that have a considerable burden if left untreated. The hypoxia-induced by the occluded vessel can disrupt the circulation of any organ, the cornerstone of treating thrombosis is rapid diagnosis and appropriate treatment. Diagnosis of thrombosis may be made by using laboratory tests or imaging techniques in individuals who have clinical manifestations of a thrombotic event. The use of serum micro ribonucleic acids (RNAs) has recently been applied to the diagnosis of thrombosis. These small RNA molecules are emerging as new diagnostic markers but have had very limited applications in vascular disease. Most of the articles provided various microRNAs with different levels of accuracy. However, there remains a lack of an appropriate panel of the most specific microRNA in the literature. The purpose of the present review was to summarize the existing data on the use of microRNAs as a diagnostic biomarker for venous thrombosis. 相似文献
63.
Ahad Ferdowsi Khosroshahi Jafar Soleimani Rad Razie Kheirjou Babak Roshangar Morteza Rashtbar Roya Salehi Mohammad Reza Ranjkesh Leila Roshangar 《Journal of cellular physiology》2020,235(2):1556-1567
The extracellular matrix of different mammalian tissues is commonly used as scaffolds in the field of tissue engineering. One of these tissues, which has frequently been studied due to its structural and biological features, is the small intestine submucosal membrane. These research are mainly done on the porcine small intestine. However, a report has recently been published about a scaffold produced from the submucosal layer of the ovine small intestine. In the present study, ovine small intestine submucosal (OSIS) was decellularized in a modified manner and its histological, morphological, and biomechanical properties were studied. Decellularization was performed in two phases: physical and chemical. In this method, a chloroform-methanol mixture, enzymatic digestion, and a constant dose of sodium dodecyl sulfate (SDS) was used in the least agitation time and its histological property and biocompatibility were evaluated in the presence of adipose tissue-derived stem cells (ADSCs); furthermore, ADSCs were isolated with a simple method (modified physical washing non-enzymatic isolation). The results were showed that the use of OSIS could be effective and operative. Mechanical properties, histological structure and shape, and glycosaminoglycan content were preserved. In the SDS-treated group, more than 90% of the native cells of tissue were deleted, and also in this group, no toxicity was observed and cell proliferation was supported, compared to the untreated group. Therefore, our results indicate that ADSCs seeded on OSIS scaffold could be used as a new approach in regenerative medicine as hybrid or hydrogel application. 相似文献
64.
Mohammad Reza Amirjani 《Archives Of Phytopathology And Plant Protection》2013,46(2):228-243
Salinity is a major yield-reducing factor in coastal and arid irrigated rice production systems. Rice seedlings (Oryza sativa cv. Tarom Atri) were exposed to different NaCl concentrations for 8 days after germination. Plants height, fresh and dry weight, relative water content, pigment and carbohydrate content, photosynthetic efficiency and lipid peroxidase and antioxidant enzyme activity of rice seedlings grown under salt stress were investigated. Seedling grown under 25and 50 mM salt were shorter than the control. They could, however, develop their secondary leaves. Seedlings grown in the nutrient solution supplied with 100 and 200 mM extra salt could not develop their secondary leaves. Fresh weight ofseedlings grown under salt stress reduced up to 42.2% of the non-treated seedlings. Chlorophylls and carotenoids contents decreased significantly in the salt-treated seedlings. Carotenoid contents in NaCl-treated seedlings were decreased to 39.3%. No significant changes occurred in the photochemical efficiency of control and stressed plants. Increasing concentrations of NaCl resulted in increase and decrease of Na+ and K+ ions, respectively. NaCl salinity caused an increase in both peroxide content and lipid peroxidation. Seedlings which recovered for 24 h showed lower peroxide and malondialdehyde content. 相似文献
65.
Leila Ebrahimi Heshmatolah Aminian Hassan Reza Etebarian Navazolah Sahebani 《Archives Of Phytopathology And Plant Protection》2013,46(17):2057-2065
In this study, Torulaspora delbrueckii alone and in combination with silicon were evaluated for the control of apple blue mould disease caused by Penicillium expansum. In vitro, the antagonistic effects of T. delbrueckii in controlling mycelial growth of P. expansum on potato-dextrose-agar (PDA) in dual cultures, and the growth of P. expansum alone with cell-free metabolites and volatile components of T. delbrueckii were assayed. In vitro, to evaluate the direct effect of silicon on mycelial growth of pathogen, silicon at different concentrations (0.2, 0.4, 0.6, 1 and 2% (wt./vol.)) was added to PDA medium. Silicon at 0.6% (wt./vol.) and above concentrations completely inhibited the mycelial growth of P. expansum. However, it had no significant effect on population dynamics of yeast in vitro and in apple wounds. In vivo, silicon at 0.2 and 1% (wt./vol.) in combination with antagonistic yeast (1 × 108 cell/ml) was a more effective approach to reduce the lesion diameter of blue mould decay of apples than the application of silicon or T. delbrueckii alone at 20 and 4°C, respectively. 相似文献
66.
Mostafa Hasanzadeh Mehrnoosh Mohammadifar Maryam Tavakol Norabadi Samaneh Dashtipoor Navazollah Sahebani Hassan Reza Etebarian 《Archives Of Phytopathology And Plant Protection》2013,46(17):2087-2094
Environmental pollution in addition to direct damage on plant growth, with the destruction of biological control agents, causes indirect damage to plants. The aim of this research was to study the effects of different concentrations (0, 500, 1000, 1500 and 2000 ppm) of heavy metals including Ag, Co, Cu, Fe, Hg, Mn, Pb and Zn on the mycelial growth and to assess the fungicidal or fungistatic effects of these salts on five Nematophagus fungi including Trichoderma harzianum (T8), Trichoderma virens (T21), Trichoderma hamatum (T9), Pochonia chlamydosporia var. chlamydosporia and Arthrobotrys oligospora. The results show that Ag, Co, Cu, Fe and Hg could stop the mycelium growth of all fungi, but Mn, Pb and Zn cannot inhibit the growth of these fungi completely. Among the first group, Hg and Cu stopped the growth of fungi even in 500 ppm. Among these metals that inhibit the growth of fungi, Cu has fungistatic effect and others have fungicide effect. The experiment was conducted in vitro condition, using potato dextrose agar (PDA) under complete randomised design with four replications. The data of mycelium growth were recorded at seven days after inoculation at 25 ± 2°C. 相似文献
67.
Characterisation of digestive protease in the rose sawfly,Arge rosae Linnaeus (Hymenoptera: Argidae)
Mahbobe Sharifi Moloud Gholamzadeh Chitgar Reza Hasan Sajedi Sudabe Amini 《Archives Of Phytopathology And Plant Protection》2013,46(10):1170-1182
Insect midgut proteases are excellent targets for insecticidal agents such as protease inhibitors. These inhibitors are used for producing transgenic plants, resistant to pests. For achieving this goal, it is necessary to find the nature of specific proteases and their properties for adopting possible pest management procedure. Therefore, characterisation of the enzymes in the gut of the rose sawfly, Arge rosae (Hymenoptera: Argidae), responsible for proteolysis, was performed using a range of synthetic substrates and specific inhibitors. The optimum conditions for general proteases and trypsin were achieved at pH 10. The highest activity for general proteases was obtained at a temperature of 45°C. The use of specific inhibitors and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) provided evidence to suggest that most of the proteases belonged to the serine group because of high inhibitory effect of phenyl methane sulfonyl fluoride on total proteolytic activity. Also, inhibition assays and zymogram analysis showed that metalloproteases are present in A. rosae digestive system. These results indicated that A. rosae larvae mainly used serine proteases for protein digestion, with chymotrypsin as the dominant form. The kinetic parameters of trypsin-like proteases using N-benzoyl-dl-arg-p-nitroanilide as substrate indicated that the K m and V max values of trypsin in the gut of the fifth instar larvae were 730 ± 17.3 μM and 456 ± 13.85 nmol min?1 mg?1 protein, respectively. 相似文献
68.
Leila Farahani Hasan Reza Etebarian Navazolah Sahebani Heshmatolah Aminian 《Archives Of Phytopathology And Plant Protection》2013,46(3):310-317
In this study, antagonistic yeast Candida membranifaciens was combined with different concentrations of silicon (Si; 0, 0.1, 0.3 and 0.5% wt/vol) to evaluate the control of blue mold of apple in storage at 20°C and 5°C. Preliminary studies showed that Si at 0.6% or above inhibited mycelial growth of pathogens significantly in vitro. In vitro studies showed that Si at 0.1% had lower effect on yeast growth. In vivo studies showed that combination of different concentrations of Si with C. membranifaciens improved the efficacy of yeast in control of disease better than Si and yeast alone (P < 0.05). Our result showed that the effective concentration of Si is varied based on pathogen isolates and temperature, so that the most effective concentration of Si was 0.5% for isolate P2 at 20°C and 0.5% and 0.1% for isolates P1 and P2 at 5°C. 相似文献
69.
Abbas Azadmehr Keyvan Alizadeh Oghyanous Reza Hajiaghaee Zahra Amirghofran Mohammad Azadbakht 《Cellular and molecular neurobiology》2013,33(8):1135-1141
In this study, the neuroprotective effect of Scrophularia striata Boiss (Scrophulariaceae) extract, a plant growing in northeastern of Iran, against oxidative stress-induced neurocytotoxicity in PC12 was evaluated. The PC12 cell line pretreated with different concentrations (10, 50, 100, and 200 μg/ml) of the extract and then treated with H2O2 to induce oxidative stress and neurotoxicity. Survival of the cells, reactive oxygen species (ROS) generation, and apoptosis were measured using MTT assay, fluorescent probe 2′,7′-dichlorofluorescein diacetate, and annexin V/propidium iodide, respectively. Moreover, the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) was used to evaluate the antioxidant capacity of the plant extract. Phytochemical assay by thin layer chromatography showed that the main components, including phenolic compounds, phenyl propanoids and flavonoids, were presented in the S. striata extract. The extract in concentrations of 50–200 μg/ml protected PC12 cells from H2O2-induced toxicity. The survival of the cells at concentration of 200 μg/ml was 64 % compared to that of H2O2 alone-treated cells (48 %) (p < 0.001). The extract also dose-dependently reduced intracellular ROS production (p < 0.001). Moreover, the extract showed antioxidative effects and decreased apoptotic cells. Collectively, these findings indicated the ability of S. striata to decrease ROS generation and cell apoptosis and also suggest the presence of the neuroprotective agents in this plant. 相似文献
70.
Seyyede Sanam Kazemi Shahandashti Reza Maali Amiri Hassan Zeinali Seyyede Sanaz Ramezanpour 《Molecular biology reports》2013,40(2):893-903
Plant cells often increase cold tolerance by reprogramming their genes expression which results in adjusted metabolic alternations, a process enhanced under cold acclimation (CA) phase. In present study, we assessed the changes of membrane fatty acid compositions and defense machine (like antioxidative enzymes) along with damage indexes like electrolyte leakage index (ELI) and malondialdehyde (MDA) during CA, cold stress (CS) and recovery (R) phases in chickpea (Cicer arietinum L.). Results showed an increase in unsaturated fatty acids ratio compare to saturated ones which is a sign of cold tolerance especially after CA phase. Antioxidant enzymes had an important role during CA and R phases while CS affected their activity which can be a sign for associating other metabolites or enzymes activities to create cold tolerance in plants. To investigation of enzymes assay under experimental treatments, the expression pattern of some enzymes including superoxide dismutase (sod), catalase (cat) and lipoxygenase (lox) was studied using quantitative real time PCR. LOX activity has shown a bilateral behavior: a positive relation with membrane damage index in CA and an interesting link with double bond index (DBI) in CS indicating probably its role in secondary metabolites like jasmonic acid signaling pathway. It was suggested that increased DBI and low LOX activity under CS could be a reason for plant cold tolerance. 相似文献