全文获取类型
收费全文 | 3158篇 |
免费 | 198篇 |
国内免费 | 2篇 |
专业分类
3358篇 |
出版年
2024年 | 3篇 |
2023年 | 12篇 |
2022年 | 46篇 |
2021年 | 71篇 |
2020年 | 32篇 |
2019年 | 51篇 |
2018年 | 74篇 |
2017年 | 50篇 |
2016年 | 100篇 |
2015年 | 172篇 |
2014年 | 203篇 |
2013年 | 224篇 |
2012年 | 311篇 |
2011年 | 252篇 |
2010年 | 202篇 |
2009年 | 167篇 |
2008年 | 215篇 |
2007年 | 198篇 |
2006年 | 175篇 |
2005年 | 147篇 |
2004年 | 147篇 |
2003年 | 132篇 |
2002年 | 87篇 |
2001年 | 68篇 |
2000年 | 60篇 |
1999年 | 46篇 |
1998年 | 12篇 |
1997年 | 19篇 |
1996年 | 14篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有3358条查询结果,搜索用时 15 毫秒
31.
32.
Kung Ahn Jin-Han Bae Kyu-Hwi Nam Chong-Eon Lee Kyung-Do Park Hak-Kyo Lee Byung-Wook Cho Heui-Soo Kim 《Genes & genomics.》2011,33(3):245-250
Quantitative analysis of horse gene expression profiles under diverse experimental conditions is limited by the lack of reliable reference genes for normalization of mRNA levels. Therefore, in this study, the expression of potential reference genes was compared between thoroughbred and Jeju native horse (Jeju pony). We compared the expression of nine genes by quantitative real-time RT-PCR in fourteen tissues between the two horse breeds and analyzed their stability using the geNorm and NormFinder programs. The data obtained in this study suggest that the UBB gene could serve as a reference gene in gene expression analysis of thoroughbred and Jeju native horses. 相似文献
33.
34.
S. O. Hwang G. H. Gil Y. J. Cho K. R. Kang J. H. Lee J. C. Bae 《Applied microbiology and biotechnology》1985,22(2):108-113
Summary A process for l-phenylalanine production was studied using a tyrosine auxotrophic regulatory mutant of Escherichia coli, resistant to both -2-thienyl-dl-alanine and p-fluoro-dl-phenylalanine. Fermentations were carried out in a 30-1 fermentor with intermittent feeding of glucose plus phosphate. The mutant accumulated l-phenylalanine in the fermentation broth up to 15 g/l at pH 7.0 and 33°C. Column chromatography on a strong cation exchanger was employed as the most effective step in the purification of l-phenyl-alanine from the broth. This step brought about 4-fold concentration of the product with 96% recovery. 相似文献
35.
36.
The production and secretion of streptokinase using OmpA signal sequence in E. coli was enhanced by removing the 13 N-terminal amino acids (SK(N13). The secretion level of SK(N13 protein into the extracellular medium was two times higher than that of wild-type streptokinase. About 4500 IU of SK(N13 protein per 1 ml LB-ampicillin medium was secreted into extracellular medium at 12 hours after induction. Fully active and enhanced extracellular preparation of the mutant streptokinase may be a potential alternative source for the simple downstream processing 相似文献
37.
Individual mouse strains differ significantly in terms of behavior and cognitive function. Strain-specific variation of metabolic
protein levels in the hippocampus among various commonly used mouse strains, however, has not been investigated yet. A proteomic
approach based on two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry [high capacity ion trap (HCT)]
has been chosen to address this question by determining strain-dependent levels of metabolic proteins in hippocampal tissue
of four inbred and one outbred mouse strain. Statistical analysis of protein spots on 2-DE gels of the individual strains
(n = 10) revealed significant strain-dependent differences in densities of 39 spots. Subsequent HCT analysis led to the identification
of 22 different metabolic proteins presenting with differential protein levels among the five mouse strains investigated.
Among those are proteins concerned with the metabolism of amino acid, nucleic acid, carbohydrate and energy. Moreover, proteins
known to play a pivotal role in the processes of learning and memory, such as calcium/calmodulin-dependent protein kinase type II alpha chain, were found to present with significant inter-strain variability, which is also in agreement with our previous reports. Strain-specific
protein levels of metabolic proteins in the mouse hippocampus may provide some insight into the molecular underpinnings and
genetic determination of strain-dependent neuronal function. Furthermore, data presented herein emphasize the significance
of the genetic background for the analysis of metabolic pathways in the hippocampus in wild-type mice as well as in gene-targeting
experiments. 相似文献
38.
Daniel C. Ilut Alexander E. Lipka Namhee Jeong Dong Nyuk Bae Dong Hyun Kim Ji Hong Kim Neelam Redekar Kiwoung Yang Won Park Sung-Taeg Kang Namshin Kim Jung-Kyung Moon M. A. Saghai Maroof Michael A. Gore Soon-Chun Jeong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2016,129(3):453-468
39.
Bae JH Alefelder S Kaiser JT Friedrich R Moroder L Huber R Budisa N 《Journal of molecular biology》2001,309(4):925-936
beta-Selenolo[3,2-b]pyrrolyl-L-alanine that mimics tryptophan with the benzene ring of the indole moiety replaced by selenophene, was incorporated into human annexin V and barstar. This was achieved by fermentation and expression in a Trp-auxotrophic Escherichia coli host strain using the selective pressure incorporation method. The seleno- proteins were obtained in yields comparable to those of the wild-type proteins and exhibit full crystallographic isomorphism to the parent proteins, but expectedly show altered absorbance profiles and quenched tryptophan fluorescence. Since the occurrence of tryptophan residues in proteins is rare, incorporation of the electron-rich selenium-containing tryptophan surrogate into proteins represents a useful supplementation and even a promising novel alternative to selenomethionine for solving the phase problem in protein X-ray crystallography. 相似文献
40.