全文获取类型
收费全文 | 46211篇 |
免费 | 3358篇 |
国内免费 | 18篇 |
专业分类
49587篇 |
出版年
2023年 | 241篇 |
2022年 | 570篇 |
2021年 | 998篇 |
2020年 | 588篇 |
2019年 | 738篇 |
2018年 | 1027篇 |
2017年 | 866篇 |
2016年 | 1459篇 |
2015年 | 2244篇 |
2014年 | 2417篇 |
2013年 | 3261篇 |
2012年 | 3749篇 |
2011年 | 3585篇 |
2010年 | 2216篇 |
2009年 | 1913篇 |
2008年 | 2723篇 |
2007年 | 2660篇 |
2006年 | 2336篇 |
2005年 | 2121篇 |
2004年 | 2017篇 |
2003年 | 1936篇 |
2002年 | 1789篇 |
2001年 | 551篇 |
2000年 | 421篇 |
1999年 | 524篇 |
1998年 | 492篇 |
1997年 | 342篇 |
1996年 | 344篇 |
1995年 | 298篇 |
1994年 | 295篇 |
1993年 | 301篇 |
1992年 | 289篇 |
1991年 | 294篇 |
1990年 | 279篇 |
1989年 | 253篇 |
1988年 | 227篇 |
1987年 | 194篇 |
1986年 | 166篇 |
1985年 | 219篇 |
1984年 | 188篇 |
1983年 | 154篇 |
1982年 | 157篇 |
1981年 | 151篇 |
1980年 | 139篇 |
1979年 | 156篇 |
1978年 | 112篇 |
1977年 | 118篇 |
1975年 | 93篇 |
1974年 | 100篇 |
1973年 | 114篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY (10(-7) M) was unable to induce ovulation and did not increase the ovulation rate in LH-stimulated ovaries. The combination of NPY (10(-7) M) and NE (10(-7) M) did not significantly increase the number of ovulations compared to that induced by NE (10(-7) M) alone. In conclusion, NPY content in the ovary is related to the estrous cycle, but NPY does not seem to have any direct effect on the ovulatory process. 相似文献
42.
Guaraná (Paullinia cupana) is a woody vine or sprawling shrub native to the central Amazon Basin. The seeds are commercially produced on some 6,000 ha in the state of Amazonas near Manaus. The principal article of commerce is an amber-colored, carbonated soft drink. It is also widely used as a high caffeine stimulant and in local medicines. The plant is monoecious and is damaged by a number of diseases, the most severe being anthracnose. Prospects are excellent for greatly expanded international markets. 相似文献
43.
44.
45.
46.
47.
Indirect evidence from observations in the field suggested thata common and often abundant cyclopoid copepod, Tropocyclopsextensus, despite its small size (0.5 mm) and largely algaldiet, is an important predator of the rotifer Polyarthra remata.Laboratory experiments showed that copepodids and adults, butnot nauplii, markedly suppressed the population growth of thisrotifer. The suppression could be attributed entirely to predation,rather than to exploitative competition for shared food resourcesor to interference. Mortality rates predicted from a separatefeeding-rate experiment weresufficient to account for the declinein population size observed in the Tropocyclops treatment ofculture experiments. Also, monitoring the availability of cryptomonadfood in treatments with and without Tropocyclops throughoutthese experiments showed that cryptomonad densities always remainedhigh and were just as high or significantly higher in the copepodtreatments. Furthermore, direct videographic observations ofP. remata from cultures with and without Tropocyclops demonstratedno significant differences in swimming velocity, tendency todeviate from a straight-line path, or frequency and length ofspontaneous escape responses. Per capita ingestion rates ofT. extensus on P. remata varied from 2 to 8 day-1, were significantlyhigher for adult females than adult males or copepodids V, andincreased significantly with rotifer density. A tendency ofcopepods to have higher ingestion rates on young than adultrotifers was not significant. Copepods cultured since birthwith cryptomonads and P. remata ate significantly fewer rotifersthan those cultured only with cryptomonads; this may be explainedby a more sated condition of the former and provided no evidencefor the idea that previous experience with this rotifer mightincrease predation efficiency. The results show that T. extensusin natural communities has the potential to deplete naturalpopulations of susceptible rotifer prey. Accordingly, it mayshift the species structure of rotifer assemblages in favourof resistant species, and provide selection pressure for avoidanceresponses. 相似文献
48.
49.
The presence of chlorophyll b and the estimation of phaeopigments in marine phytoplankton 总被引:1,自引:0,他引:1
A reverse-phase h.p.l.c. technique was used to estimate theconcentration of chlorophyll b in phytoplankton cultures, fecalpellets of Calanus pacificus, and suspended paniculate matterfrom the Central North Pacific, Oregon coastal waters, and DabobBay (a temperate fjord in Puget Sound, WA, USA). The purposewas to assess the distribution of this pigment in the euphoticzone and its effect on the fluorometnc estimation of phaeopigments.Analyses of natural waters confirm high chlorophyll b concentrations(median mass ratio of b:a > 0.3) at the depth of the chlorophylla maximum in tropical waters while values for temperate planktonare relatively low (median mass ratio of chl b:a = 0.05) andpatchy. Zooplankton fecal pellets showed a significant enrichmentin chlorophyll b, suggesting grazing as a mechanism to explainhigh concentrations of this pigment at the bottom of the euphoticzone. It is estimated that the presence of chlorophyll b couldcause an average overestimation of phaeopigment concentrationby the fluorometnc technique of 38% between 0 and 200 m in theCentral North Pacific. This effect is more pronounced at thelayer of chlorophyll b maximum (120140 m).
1Present address: Marine Biology Research Division, A-002, ScrippsInstitution of Oceanography, La Jolla, CA 92093, USA 相似文献
50.
Taurine entered the alga Chlorella fusca Shihira et Krauss strain 21l-8b via a pH and energy-dependent system ("permease"). Transport followed triphasic kinetics from 10−6 to 10−2 M with Km values for taurine of 5.4 × 10−5 , 4.1 × l0−4 and l.5 × 10−3 M. This uptake system was specific for sulfonic acids and showed no affinity for α- and β -amino acids or Na+ ; thus the permease of C. fusca is different from all known taurine transport systems with respect to structural specificity and lack of Na+ -dependence. Uptake was not observed in sulfate-grown algae but developed as a response to sulfate limitation within 2 h. Sulfate addition caused a rapid decline in taurine transport capacity. Labeled taurine was rapidly metabolized in C. fusca to sulfate and ethanolamine, suggesting oxidative hydrolysis as the mechanism of C-S bond cleavage. Further incorporation of these catabolic products in C - and S -metabolism was demonstrated. Taurine catabolism was also detected in other green algae and some cyanobacteria. 相似文献