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排序方式: 共有204条查询结果,搜索用时 15 毫秒
191.
Krause M Leslie JD Stewart M Lafuente EM Valderrama F Jagannathan R Strasser GA Rubinson DA Liu H Way M Yaffe MB Boussiotis VA Gertler FB 《Developmental cell》2004,7(4):571-583
Lamellipodial protrusion is regulated by Ena/VASP proteins. We identified Lamellipodin (Lpd) as an Ena/VASP binding protein. Both proteins colocalize at the tips of lamellipodia and filopodia. Lpd is recruited to EPEC and Vaccinia, pathogens that exploit the actin cytoskeleton for their own motility. Lpd contains a PH domain that binds specifically to PI(3,4)P2, an asymmetrically localized signal in chemotactic cells. Lpd's PH domain can localize to ruffles in PDGF-treated fibroblasts. Lpd overexpression increases lamellipodial protrusion velocity, an effect observed when Ena/VASP proteins are overexpressed or artificially targeted to the plasma membrane. Conversely, knockdown of Lpd expression impairs lamellipodia formation, reduces velocity of residual lamellipodial protrusion, and decreases F-actin content. These phenotypes are more severe than loss of Ena/VASP, suggesting that Lpd regulates other effectors of the actin cytoskeleton in addition to Ena/VASP. 相似文献
192.
Exposure of lactogen-dependent (Nb2-11C) and lactogen independent (Nb2-SP) lymphoma cells to cholera toxin (0.05-50 pM) resulted within 18-28 h in a 50% decrease in the binding capacity of the intact cells to iodinated human growth hormone, and 40% decrease in cell-homogenates. Scatchard analysis revealed that the reduction in binding resulted from loss of cell-surface receptors accompanied by degradation of intracellular receptors. No alterations in receptor binding affinity were observed. One to 3 h of exposure to the toxin was sufficient to reduce the binding to the level obtained after continuous incubation with the toxin for 28 h. Addition of dibutyryl cAMP (0.1mM) to the medium resulted in similar down-regulation of lactogenic receptors. 相似文献
193.
Auerbuch V Loureiro JJ Gertler FB Theriot JA Portnoy DA 《Molecular microbiology》2003,49(5):1361-1375
The Listeria monocytogenes surface protein ActA mediates actin-based motility by interacting with a number of host cytoskeletal components, including Ena/VASP family proteins, which in turn interact with actin and the actin-binding protein profilin. We employed a bidirectional genetic approach to study Ena/VASP's contribution to L. monocytogenes movement and pathogenesis. We generated an ActA allelic series within the defined Ena/VASP-binding sites and introduced the resulting mutant L. monocytogenes into cell lines expressing different Ena/VASP derivatives. Our findings indicate that Ena/VASP proteins contribute to the persistence of both speed and directionality of L. monocytogenes movement. In the absence of the Ena/VASP proline-rich central domain, speed consistency decreased by sixfold. In addition, the Ena/VASP F-actin-binding region increased directionality of bacterial movement by fourfold. We further show that both regions of Ena/VASP enhanced L. monocytogenes cell-to-cell spread to a similar degree, although the Ena/VASP F-actin-binding region did so in an ActA-independent manner. Surprisingly, our ActA allelic series enabled us to uncouple L. monocytogenes speed from directionality although both were controlled by Ena/VASP proteins. Lastly, we showed the pathogenic relevance of these findings by the observation that L. monocytogenes lacking ActA Ena/VASP-binding sites were up to 400-fold less virulent during an adaptive immune response. 相似文献
194.
Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility 总被引:42,自引:0,他引:42
Bear JE Svitkina TM Krause M Schafer DA Loureiro JJ Strasser GA Maly IV Chaga OY Cooper JA Borisy GG Gertler FB 《Cell》2002,109(4):509-521
Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP proteins accumulate in protruding lamellipodia and promote the rapid actin-driven motility of the pathogen Listeria. In contrast, Ena/VASP negatively regulate cell translocation. To resolve this paradox, we analyzed the function of Ena/VASP during lamellipodial protrusion. Ena/VASP-deficient lamellipodia protruded slower but more persistently, consistent with their increased cell translocation rates. Actin networks in Ena/VASP-deficient lamellipodia contained shorter, more highly branched filaments compared to controls. Lamellipodia with excess Ena/VASP contained longer, less branched filaments. In vitro, Ena/VASP promoted actin filament elongation by interacting with barbed ends, shielding them from capping protein. We conclude that Ena/VASP regulates cell motility by controlling the geometry of actin filament networks within lamellipodia. 相似文献
195.
Ally S Sauer NJ Loureiro JJ Snapper SB Gertler FB Goldberg MB 《Cellular microbiology》2004,6(4):355-366
Shigella move through the cytosol of infected cells by assembly of a propulsive actin tail at one end of the bacterium. Vasodilator-stimulated phosphoprotein (VASP), a member of the Ena/VASP family of proteins, is important in cellular actin dynamics and is present on intracellular Shigella. VASP binds both profilin, an actin monomer-binding protein, and vinculin, a component of intercellular contacts that also binds the Shigella actin assembly protein IcsA. It has been postulated that VASP might serve as a linker between vinculin and profilin on intracellular Shigella, thereby delivering profilin to the Shigella actin assembly machinery. We show that Shigella actin-based motility is unaltered in cells that are deficient for the Ena/VASP family of proteins. In these cells, Shigella form normal-appearing actin tails and move at rates that are comparable to the rates of bacterial movement in Ena/VASP-deficient cells complemented with the Ena/VASP family member Mena. Finally, whereas vinculin can bind the Arp2/3 complex, we show that Arp2/3 recruitment to Shigella is not correlated with vinculin recruitment, indicating that the role of vinculin in Shigella motility is not recruitment of Arp2/3. Thus, although VASP is recruited to the surface of intracellular Shigella, it is not essential for Shigella actin-based motility. 相似文献
196.
Ena/VASP proteins have an anti-capping independent function in filopodia formation 总被引:5,自引:0,他引:5 下载免费PDF全文
Applewhite DA Barzik M Kojima S Svitkina TM Gertler FB Borisy GG 《Molecular biology of the cell》2007,18(7):2579-2591
Filopodia have been implicated in a number of diverse cellular processes including growth-cone path finding, wound healing, and metastasis. The Ena/VASP family of proteins has emerged as key to filopodia formation but the exact mechanism for how they function has yet to be fully elucidated. Using cell spreading as a model system in combination with small interfering RNA depletion of Capping Protein, we determined that Ena/VASP proteins have a role beyond anticapping activity in filopodia formation. Analysis of mutant Ena/VASP proteins demonstrated that the entire EVH2 domain was the minimal domain required for filopodia formation. Fluorescent recovery after photobleaching data indicate that Ena/VASP proteins rapidly exchange at the leading edge of lamellipodia, whereas virtually no exchange occurred at filopodial tips. Mutation of the G-actin-binding motif (GAB) partially compromised stabilization of Ena/VASP at filopodia tips. These observations led us to propose a model where the EVH2 domain of Ena/VASP induces and maintains clustering of the barbed ends of actin filaments, which putatively corresponds to a transition from lamellipodial to filopodial localization. Furthermore, the EVH1 domain, together with the GAB motif in the EVH2 domain, helps to maintain Ena/VASP at the growing barbed ends. 相似文献
197.
Recombinant carp growth hormone and its Cys123Ala analogue were refolded in 4.5 M urea, pH 11.3 in the presence of 0.1 mM cysteine. Shortening of the refolding process from 48 h to 1 h resulted in a 30 to 40 fold increase in yield of the biologically active monomers, and lowered dimerization and oligomerization. A similar short-time refolding procedure was also found to be advantageous with other structurally different, non-related proteins, such as the extracellular domains of rabbit and bovine prolactin receptors. 相似文献
198.
S Gertler N M Young 《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,102(2):377-381
1. Three aspects of the monoclonal rat IgA IR22 were investigated: its free thiol groups, its hinge structure and its glycopeptides. 2. Exposed thiol groups, 1.5 per IgA dimer, were located in the Fc region, and buried thiol groups, 3.2 per IgA dimer, were located in the Fd region. 3. Rat IgA was cleaved to Fab fragments by pepsin, but, unlike mouse IgA, it was not cleaved at the hinge region by papain. 4. The contents of GlcNAc indicated that there were two N-linked glycopeptides in the Fab region, and one in the Fc or tail regions. 相似文献
199.
A transient cancer scare is presented as a rare opportunity to observe the effect of a perceived increase in risk on the price of residential property. The temporary nature of the perceived excess risk allows for the isolation of a risk premium from the change in housing prices, because prices decline during this natural experiment and return to normal when the cancer scare is proven to be unfounded. Value of life measures imputed from this risk premium are orders of magnitude lower than similar values obtained by studies involving other risk-dollar tradeoffs. The likelihood of death which is taken to be valued by the risk premium is much greater in this case than in other value-of-life studies, giving support to the notion that the aversion to risk is not directly proportional to the probability of harm. 相似文献
200.