全文获取类型
收费全文 | 2798篇 |
免费 | 210篇 |
出版年
2022年 | 12篇 |
2021年 | 25篇 |
2020年 | 22篇 |
2019年 | 19篇 |
2018年 | 38篇 |
2017年 | 39篇 |
2016年 | 46篇 |
2015年 | 92篇 |
2014年 | 110篇 |
2013年 | 119篇 |
2012年 | 180篇 |
2011年 | 177篇 |
2010年 | 129篇 |
2009年 | 118篇 |
2008年 | 164篇 |
2007年 | 139篇 |
2006年 | 163篇 |
2005年 | 149篇 |
2004年 | 150篇 |
2003年 | 142篇 |
2002年 | 131篇 |
2001年 | 79篇 |
2000年 | 68篇 |
1999年 | 39篇 |
1998年 | 40篇 |
1997年 | 39篇 |
1996年 | 37篇 |
1995年 | 23篇 |
1994年 | 25篇 |
1993年 | 29篇 |
1992年 | 35篇 |
1991年 | 36篇 |
1990年 | 37篇 |
1989年 | 31篇 |
1988年 | 28篇 |
1987年 | 15篇 |
1986年 | 23篇 |
1985年 | 19篇 |
1984年 | 19篇 |
1983年 | 15篇 |
1982年 | 16篇 |
1981年 | 16篇 |
1980年 | 12篇 |
1979年 | 16篇 |
1978年 | 14篇 |
1977年 | 20篇 |
1976年 | 10篇 |
1975年 | 11篇 |
1974年 | 15篇 |
1973年 | 15篇 |
排序方式: 共有3008条查询结果,搜索用时 625 毫秒
991.
992.
Capsule: Foraging behaviour in the Razorbill Alca torda during breeding was similar to that found elsewhere, aside from dive shape.Aims: To investigate the foraging behaviour of Razorbills during the breeding season at the largest colony in the central Baltic Sea.Methods: A combination of global positioning system (GPS) and time-depth recorder (TDR) devices were used on Razorbills breeding on the island of Stora Karlsö, Baltic Sea, during the chick-rearing period.Results: Five GPS tracks and nine TDR logs were retrieved from 12 Razorbills, and 7399 dives were analysed. Razorbills foraged south and southwest of the colony. Maximum and mean (±sd) foraging range from the colony was 72.7?km and 13.1?±?13.5?km, respectively. Mean dive depth (15.3?±?2.4?m) and duration (53.1?±?8.5?s) were similar to those of a more southern Baltic Sea Razorbill colony. Dive depth had a bimodal distribution, with 70% of dives deeper than 10?m and 30% shallower than 10?m. There was a clear diel foraging pattern with 89% of dives occurring during daytime and a higher proportion of shallow dives at night. Unexpectedly, dives were primarily U-shaped. The Razorbills spent 31% of their overall time activity budget flying or diving.Conclusion: Aside from dive shape, foraging behaviour was consistent with that reported at other colonies of Razorbills. Inconsistency in dive shape may be due to a bimodal foraging strategy, local prey behaviour or competition with the Common Guillemot Uria aalge. 相似文献
993.
Fernando J. Sialana Anally Ribeiroda Silva Menegasso Roman Smidak Ahmed M. Hussein Michael Zavadil Thomas Rattei Gert Lubec Mario Sergio Palma Jana Lubec 《Proteomics》2019,19(13)
The olfactory conditioning of the bee proboscis extension reflex (PER) is extensively used as a paradigm in associative learning of invertebrates but with limited molecular investigations. To investigate which protein changes are linked to olfactory conditioning, a non‐sophisticated conditioning model is applied using the PER in the honeybee (Apis mellifera). Foraging honeybees are assigned into three groups based on the reflex behavior and training: conditioned using 2‐octanone (PER‐conditioned), and sucrose and water controls. Thereafter, the brain synaptosomal proteins are isolated and analyzed by quantitative proteomics using stable isotope labeling (TMT). Additionally, the complex proteome dataset of the bee brain is generated with a total number of 5411 protein groups, including key players in neurotransmitter signaling. The most significant categories affected during olfactory conditioning are associated with “SNARE interactions in vesicular transport” (BET1 and VAMP7), ABC transporters, and fatty acid degradation pathways. 相似文献
994.
Gert‐Jan Jeunen Michael Knapp Hamish G. Spencer Helen R. Taylor Miles D. Lamare Michael Stat Michael Bunce Neil J. Gemmell 《Ecology and evolution》2019,9(3):1323-1335
DNA extraction from environmental samples (environmental DNA; eDNA) for metabarcoding‐based biodiversity studies is gaining popularity as a noninvasive, time‐efficient, and cost‐effective monitoring tool. The potential benefits are promising for marine conservation, as the marine biome is frequently under‐surveyed due to its inaccessibility and the consequent high costs involved. With increasing numbers of eDNA‐related publications have come a wide array of capture and extraction methods. Without visual species confirmation, inconsistent use of laboratory protocols hinders comparability between studies because the efficiency of target DNA isolation may vary. We determined an optimal protocol (capture and extraction) for marine eDNA research based on total DNA yield measurements by comparing commonly employed methods of seawater filtering and DNA isolation. We compared metabarcoding results of both targeted (small taxonomic group with species‐level assignment) and universal (broad taxonomic group with genus/family‐level assignment) approaches obtained from replicates treated with the optimal and a low‐performance capture and extraction protocol to determine the impact of protocol choice and DNA yield on biodiversity detection. Filtration through cellulose‐nitrate membranes and extraction with Qiagen's DNeasy Blood & Tissue Kit outperformed other combinations of capture and extraction methods, showing a ninefold improvement in DNA yield over the poorest performing methods. Use of optimized protocols resulted in a significant increase in OTU and species richness for targeted metabarcoding assays. However, changing protocols made little difference to the OTU and taxon richness obtained using universal metabarcoding assays. Our results demonstrate an increased risk of false‐negative species detection for targeted eDNA approaches when protocols with poor DNA isolation efficacy are employed. Appropriate optimization is therefore essential for eDNA monitoring to remain a powerful, efficient, and relatively cheap method for biodiversity assessments. For seawater, we advocate filtration through cellulose‐nitrate membranes and extraction with Qiagen's DNeasy Blood & Tissue Kit or phenol‐chloroform‐isoamyl for successful implementation of eDNA multi‐marker metabarcoding surveys. 相似文献
995.
The stratum corneum (SC) plays a fundamental role in the barrier function of the skin. The SC consists of corneocytes embedded in a lipid matrix. The main lipid classes in the lipid matrix are ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs). The aim of this study was to examine the effect of the chain length of FFAs on the thermotropic phase behavior and mixing properties of SC lipids. Fourier transform infrared spectroscopy and Raman imaging spectroscopy were used to study the mixing properties using either protonated or deuterated FFAs. We selected SC model lipid mixtures containing only a single CER, CHOL and either a single FFA or a mixture of FFAs mimicking the FFA SC composition. The single CER consists of a sphingoid base with 18 carbon atoms and an acyl chain with a chain length of 24 carbon atoms. When using lignoceric acid (24 carbon atoms) or a mixture of FFAs, the CER and FFAs participated in mixed crystals, but hydration of the mixtures induced a slight phase separation between CER and FFA. The mixed crystalline structures did not phase separate during storage even up to a time period of 3 months. When using palmitic acid (16 carbon atoms), a slight phase separation was observed between FFA and CER. This phase separation was clearly enhanced during hydration and storage. In conclusion, the thermotropic phase behavior and the mixing properties of the SC lipid mixtures were shown to strongly depend on the chain length and chain length distribution of FFAs, while hydration enhanced the phase separation. 相似文献
996.
997.
998.
Henry Moll Laura Lütke Velina Bachvarova Andrea Cherkouk Sonja Selenska-Pobell Gert Bernhard 《Geomicrobiology journal》2014,31(8):682-696
Bacterial cell walls have great potential to influence the speciation and mobility of actinides and lanthanides in the environment. In this study we explored the unknown interaction between Cm(III)/Eu(III) and cell-suspensions of Sporomusa sp. MT-2.99, a novel isolate recovered from Opalinus Clay (Mont Terri, Switzerland). The Cm(III)/Eu(III) binding by the cell surface functional groups was studied by potentiometry combined with time-resolved laser-induced fluorescence spectroscopy (TRLFS). This article provides stability constants of Cm(III)/Eu(III) complexed by cell surface functional groups. We could show that as a function of pH Cm(III)/Eu(III) binding occurred to hydrogen phosphoryl, carboxyl and deprotonated phosphoryl sites. Both metals showed a similar interaction process consisting of surface complexation (major) with high thermodynamic stability and an irreversible binding within the cell envelope (minor). 相似文献
999.
Hazem M. Kalaji Gert Schansker Richard J. Ladle Vasilij Goltsev Karolina Bosa Suleyman I. Allakhverdiev Marian Brestic Filippo Bussotti Angeles Calatayud Piotr Dąbrowski Nabil I. Elsheery Lorenzo Ferroni Lucia Guidi Sander W. Hogewoning Anjana Jajoo Amarendra N. Misra Sergio G. Nebauer Simonetta Pancaldi Consuelo Penella DorothyBelle Poli Martina Pollastrini Zdzislawa B. Romanowska-Duda Beata Rutkowska João Serôdio Kancherla Suresh Wiesław Szulc Eduardo Tambussi Marcos Yanniccari Marek Zivcak 《Photosynthesis research》2014,122(2):121-158
The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists. 相似文献
1000.
Peggy J. de Vos van Steenwijk Mariette I. E. van Poelgeest Tamara H. Ramwadhdoebe Margriet J. G. Löwik Dorien M. A. Berends-van der Meer Caroline E. van der Minne Nikki M. Loof Linda F. M. Stynenbosch Lorraine M. Fathers A. Rob P. M. Valentijn Jaap Oostendorp Elisabeth M. Osse Gert Jan Fleuren Linda Nooij Marjolein J. Kagie Bart W. J. Hellebrekers Cornelis J. M. Melief Marij J. P. Welters Sjoerd H. van der Burg Gemma G. Kenter 《Cancer immunology, immunotherapy : CII》2014,63(2):147-160
The capacity of a low-dose HPV16 synthetic long-peptide vaccine (HPV16-SLP) to induce an HPV16-specific T-cell response as well as to establish long-term immunologic memory in patients with low-grade abnormalities of the cervix was determined in a placebo-controlled, double-blinded phase II study. In addition, the effect of a booster vaccination after 1 year was evaluated. Patients received either the HPV16-SLP or a placebo at the start of the study. After 1 year, the vaccinated patients were again randomized to receive the HPV16-SLP or a placebo. Patients were followed for 2 years. HPV16-specific T-cell responses were determined in pre- and post-vaccination blood samples by ELISPOT, proliferation assay and cytokine assays. We show that the HPV16-specific T-cell responses detected after vaccination are clearly due to vaccination and that reactivity was maintained for at least 2 years. Interestingly, a booster vaccination after 1 year especially augmented the HPV16-specific Th2 response. Furthermore, pre-existing immunity to HPV16 was associated with a stronger response to vaccination and with more side effects, reflected by flu-like symptoms. We conclude that two low-dose injections of HPV16-SLP can induce a strong and stable HPV16-specific T-cell response that lasts for at least 1 year. If booster vaccination is required, then polarizing adjuvant should be added to maintain the Th1 focus of the vaccine-induced T-cell response. 相似文献