Antisense EGFR sequence reverses the growth properties of human liver carcinoma cell line BEL-7404 in vitro

A recombinant plasmid containing a full length human epidermal growth factor receptor (EGFR) cDNA sequence in antisense orientation was transferred into cells of a human liver carcinoma cell line BEL-7404. Compared with the control cell clone JX-0 transferred with the vector plasmid and the parent BEL-7404 cells, the antisense EGFR transferred cell clone JX-1 showed a decreased EGFR gene expression and reduced significantly the growth potential either in anchorage-dependent or anchorage-independent growth. Furthermore, JX-1 cells appeared to be distinctly dependent on serum concentration for monolayer growth. The results suggested that antisense EGFR could partly block the EGFR gene expression and reverse the malignant growth properties of human liver carcinoma cells in vitro.     

Cytoskeletal arrangement and its intercellular connec-tion in wheat young leaf cells

By using the techniques of partial digestion of cell wall and selective extraction,we examined the cytoskeleton of wheat yong leaf cells under scanning electron microscope(SEM).A 3-dimensional cytoskeletal system,showing some new features,was observed.The cortical network located beneath the cross wall was an anastomosing organization.The association of nucleus with the cell wall by some skeletal filaments was also found.It is notice able that there were cytoskeletal filaments,which passed through cell wall and connected together with cytoskeletal arrays of adjacent cells,Thus,it is possible that an integral skeletal network existed within the yong leaf tissue of wheat.     

菰属系统与演化研究—胚形态

本文采用光学显微镜对全世界菰属４种２亚种及其有关属种共４属７种的胚形态进行系统研究后,获得菰属Ｚｉｚａｎｉａ Ｌ．与山涧草Ｃｈｉｋｕｓｉｃｈｌｏａ ａｐｕａｔｉｃａ Ｋｏｉｄｚ．的胚型为Ｆ＋ＦＰ,稻Ｏｒｙｚａ ｓａｔｉｖａ Ｌ．与拟菰Ｚｉｚａｎｉｏｐｓｉｓ ｍｉｌｉａｃｅａ（Ｍｉｃｈｘ．）Ｄｏｅｌｌ ＆ Ａｓｃｈｅｒｓ．的胚型为Ｆ＋ＦＰ,其中山涧草的胚型为首次报导。根据胚型,这４属均应位在禾本科Ｇ     

中国栗米草属植物的校订

本文校订了中国粟米属植物,共有4种。国产的粟米草应是Mollugo stricta L.,而不是M.pentaphylla L.;多棱粟米草M.costataY.T.Chang et C.F.Wei是种棱粟米草M.verticillata L.的异名     

中国蒿属植物比较形态和解剖学研究——(Ⅰ)叶表皮结构

本文通过光学显微镜和扫描电镜,观察国产菊科蒿属2亚属7组30种代表植物成熟叶片的表皮细胞、气孔器、表皮毛的特征和气孔形状、气孔外拱盖及拱盖内缘、角质膜和蜡质纹饰。其中表皮细胞多角(边)形,大小不等,垂周壁平直、弓形,或者表皮细胞形状不规则,大小不等,垂周壁波浪状或波纹状。下表皮均有气孔器,上表皮有或无气孔器,气孔器类型有无规则型。无规则四细胞型、不等细胞型、十字型、横列型和平列型。大多数种类具多细胞单列毛。多细胞双列毛和腺毛仅发现在腺毛蒿组。这些微形态特征在组间存在着某些差异,具有一定的分类学和生态学意义。     

H．史密斯采集的中国紫草科植物

自H．Smith在二十,三十年代从我国采集的紫草科标本中鉴定出15属,36种,4变种,均属紫草亚科,其中包括附地菜属一新种,其中,这批标本本来应该包括更多的新种,如Johnston在1928年及以后描述的Microula rockii Johnst.,Onosma mertensioides Johnst.,O.adenopus Johnst.,Trionotis floribunda Johnst.,Kitagawa在六十年代描述的Lappula shanhsiensis Kitag.,本文作者和王庆瑞在八十年代描述的Microula pseudotrichocarpa W.T.Wang,M.turbinata W.T.Wang,M.turbinata W.T.Wang,M.floribunda W.T.Wang,Trigontis longipes W.T.Wang,T.corispermoides C.J.wang。只是由于这批柴草科标本未得到及时鉴定,才推迟了这10个种的发表。     

中国金发草属与觿茅属的新分类等级

本文报导金发草属Pogonatherum Beauv.中1新种,即二芒金发草P.biaristatum S.L.Chen et G.Y.Sheng及觿茅属Dimeria R.Br.中2新种,即异花觿茅D.heterantha S.L.Chen et G.Y.Sheng及广西觿茅D.guangxiensis S.L.Chen et G.Y.Sheng.     

应用FIA型微生物传感器测定谷氨酸含量的研究

目前应用酶或微生物细胞作为分子识别元件构建生物传感器测定谷氨酸含量的研究引起了广泛的兴趣,并陆续有实例报道。在这些报道中人们依据不同的酶催反应选择相应的离子选择性电极,如CO₂电极、NH₄⁺电极等,而测量对象有直接的测定谷氨酸,也有测定谷氨酸单钠的间接测定法。本文选用了可固定大量细胞的固定化细胞柱与流动注入法相结合的测量方法,并根据细胞柱的动力学模型分析动态响应曲线,计算测量结果,提高了测量精度,扩大了测量范围。     

中国短痣蚜科新属新种记述（同翅目：蚜总科）

本文记述了短痣蚜科１新属——环短痣蚜属ＫｒｉｋｏａｎｏｅｃｉａＺｈａｎｇｅｔＱｉａｏ,ｇｅｎ．ｎ．及１新种——环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａｃｉｒｃｕｌａＱｉａｏｅｔＺｈａｎｇ,ｓｐ．ｎ．,并探讨了该属的分类地位。模式标本存于中国科学院动物研究所昆虫标本馆内。环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａＺｈａｎｇｅｔＱｉａｏ,新属模式种：环短痣蚜ＫｒｉｋｏａｎｏｅｃｉａｃｉｒｃｕｌａＱｉａｏｅｔＺｈａｎｇ,ｓｐ．ｎ．根据环短痣蚜新属腹部有缘瘤,有翅蚜前翅中脉分岔一次等特征,该新属应归于短痣蚜亚科内,与伪短痣蚜属ＡｉｃｅｏｎａＴａｋａｈａｓｈｉ,１９２１明显不同,而与短痣蚜属ＡｎｏｅｃｉａＫｏｃｈ,１７７５有较近的亲缘关系,不同则在于：新属次生感觉圈环形（后者卵圆形或椭圆形）;原生感觉圈有睫（无睫）;体背毛少（体背毛多）;体背无大型褐色斑纹（有）;前翅翅痣狭长,近长平行四边形（近三角形或椭圆形）。就次生感觉圈的形状,原生感觉圈有无睫,跗节Ｉ毛数的进化方向（Ｓｈａｐｏｓｈｎｉｋｏｖ,１９８１）,认为新属较短痣蚜属更为进化。分布：甘肃岷县（西寨２３００ｍ）。正模：有翅孤雌蚜Ｎｏ．８７３３－２－１－１,甘肃岷县（西寨２３００