全文获取类型
收费全文 | 696篇 |
免费 | 95篇 |
出版年
2022年 | 6篇 |
2021年 | 7篇 |
2019年 | 11篇 |
2018年 | 13篇 |
2017年 | 9篇 |
2016年 | 14篇 |
2015年 | 13篇 |
2014年 | 17篇 |
2013年 | 29篇 |
2012年 | 41篇 |
2011年 | 47篇 |
2010年 | 14篇 |
2009年 | 32篇 |
2008年 | 19篇 |
2007年 | 26篇 |
2006年 | 26篇 |
2005年 | 22篇 |
2004年 | 13篇 |
2003年 | 17篇 |
2002年 | 24篇 |
2001年 | 13篇 |
2000年 | 26篇 |
1999年 | 15篇 |
1997年 | 6篇 |
1996年 | 7篇 |
1995年 | 9篇 |
1993年 | 9篇 |
1992年 | 15篇 |
1991年 | 16篇 |
1990年 | 9篇 |
1989年 | 11篇 |
1988年 | 10篇 |
1987年 | 13篇 |
1986年 | 10篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 5篇 |
1982年 | 14篇 |
1981年 | 10篇 |
1980年 | 8篇 |
1979年 | 15篇 |
1978年 | 6篇 |
1977年 | 12篇 |
1976年 | 8篇 |
1975年 | 10篇 |
1974年 | 11篇 |
1973年 | 6篇 |
1971年 | 10篇 |
1970年 | 7篇 |
1969年 | 6篇 |
排序方式: 共有791条查询结果,搜索用时 15 毫秒
111.
Julie A. Coetzee 《Biocontrol Science and Technology》2012,22(4):419-427
Meteorological weather station data are often used in climate matching studies to predict potential distributions of biological control agents, yet, this does not take into account the effects of microclimates experienced by the agents. Comparisons of the number of generations that the mirid, Eccritotarsus catarinensis, a biological control agent of water hyacinth, Eichhornia crassipes (Mart.) Solms-Laub (Pontederiaceae), was predicted to complete using meteorological weather station data, on site air temperature and water hyacinth canopy microclimate temperatures recorded over two years showed that there were no significant differences between the temperature data sources. Therefore, meteorological weather station data used in degree-day models of biological control agents are useful in explaining broad establishment patterns. 相似文献
112.
113.
Dialysis culture of microorganisms: design, theory, and results 总被引:15,自引:0,他引:15
114.
Terrestrial protected areas (PAs) are cornerstones of global biodiversity conservation. Their efficacy in terms of maintaining biodiversity is, however, much debated. Studies to date have been unable to provide a general answer as to PA conservation efficacy because of their typically restricted geographic and/or taxonomic focus, or qualitative approaches focusing on proxies for biodiversity, such as deforestation. Given the rarity of historical data to enable comparisons of biodiversity before/after PA establishment, many smaller scale studies over the past 30 years have directly compared biodiversity inside PAs to that of surrounding areas, which provides one measure of PA ecological performance. Here we use a meta-analysis of such studies (N = 86) to test if PAs contain higher biodiversity values than surrounding areas, and so assess their contribution to determining PA efficacy. We find that PAs generally have higher abundances of individual species, higher assemblage abundances, and higher species richness values compared with alternative land uses. Local scale studies in combination thus show that PAs retain more biodiversity than alternative land use areas. Nonetheless, much variation is present in the effect sizes, which underscores the context-specificity of PA efficacy. 相似文献
115.
116.
Takao Hikita Fatemeh Mirzapourshafiyi Pedro Barbacena Meghan Riddell Ayesha Pasha Mengnan Li Takuji Kawamura Ralf P Brandes Tomonori Hirose Shigeo Ohno Holger Gerhardt Michiyuki Matsuda Claudio A Franco Masanori Nakayama 《EMBO reports》2018,19(9)
Impaired cell polarity is a hallmark of diseased tissue. In the cardiovascular system, laminar blood flow induces endothelial planar cell polarity, represented by elongated cell shape and asymmetric distribution of intracellular organelles along the axis of blood flow. Disrupted endothelial planar polarity is considered to be pro‐inflammatory, suggesting that the establishment of endothelial polarity elicits an anti‐inflammatory response. However, a causative relationship between polarity and inflammatory responses has not been firmly established. Here, we find that a cell polarity protein, PAR‐3, is an essential gatekeeper of GSK3β activity in response to laminar blood flow. We show that flow‐induced spatial distribution of PAR‐3/aPKCλ and aPKCλ/GSK3β complexes controls local GSK3β activity and thereby regulates endothelial planar polarity. The spatial information for GSK3β activation is essential for flow‐dependent polarity to the flow axis, but is not necessary for flow‐induced anti‐inflammatory response. Our results shed light on a novel relationship between endothelial polarity and vascular homeostasis highlighting avenues for novel therapeutic strategies. 相似文献
117.
Mark A. Bee Carol E. Kozich Kellee J. Blackwell & H. Carl Gerhardt 《Ethology : formerly Zeitschrift fur Tierpsychologie》2001,107(1):65-84
Individuals of many territorial species discriminate between familiar territorial neighbors and unfamiliar strangers based on individual differences in acoustic signals. Many anuran amphibians are territorial and use primarily acoustic communication during social interactions, but evidence for acoustically mediated individual discrimination is available only for one species. As a first step in research designed to investigate individual discrimination in a second species of territorial frog, we examined patterns of within-male and among-male variability in 198 advertisement calls of 20 male green frogs, Rana clamitans . The aim was to determine which acoustic properties could be used by males to recognize their territorial neighbors and to estimate limits of reliability afforded by these properties for identifying different neighbors. All of the call properties that we examined exhibited significant inter-individual variation. Discriminant function analyses assigned between 52% and 100% of calls to the correct individual, depending on sample size and the call properties included in the model. This suggests that there is sufficient among-male variability to statistically identify individuals by their advertisement calls. The call properties of fundamental frequency and dominant frequency contributed the most towards discrimination among individuals. Based on their natural history and behavior and the results reported here, we suggest that male green frogs likely discriminate between strangers and adjacently territorial neighbors based on individual variation in advertisement calls. 相似文献
118.
Gerhardt F. Riedel 《Journal of phycology》1984,20(4):496-500
The acute toxicity of Cr(VI) to the diatom Thalassiosira pseudonana (Hasle and Heimdal) clone 3H was determined in artificial media of 3.2 and 0.32 ppt salinity and with variations of sulfate concentration in the media independent of salinity. Inhibitory concentrations of Cr(VI) ranged from 6.6 μM for growth rate and 4.9 μM for cell yield at 3.2 ppt salinity and 2.8 μM sulfate to 0.04 μM for growth rate and 0.02 μM for cell yield at 0.32 ppt salinity and 0.019 mM sulfate. The inhibition by Cr(VI) was a function of the ratio of Cr(VI) to sulfate. Inhibition occurred when-this ratio exceeded about 500:1. It is suggested that the mechanism for the toxicity of Cr(VI) to diatoms and perhaps other aquatic organisms involves a site at which sulfate and chromate compete. 相似文献
119.
Dolphine A Amenya Lizette L Koekemoer Ashley Vaughan John C Morgan Basil D Brooke Richard H Hunt Hilary Ranson Janet Hemingway Maureen Coetzee 《DNA sequence》2005,16(6):437-445
Pyrethroid resistance has been demonstrated in populations of Anopheles funestus from South Africa and southern Mozambique. Resistance is associated with elevated P450 monooxygenase enzymes. In this study, degenerate primers based on conserved regions of Anopheles gambiae P450 CYP4, 6 and 9 families were used to amplify genomic and cDNA templates from A. funestus. A total of 12 CYP4, 12 CYP6 and 7 CYP9 partial genes have been isolated and sequenced. BLAST results revealed that A. funestus P450s generally have a high sequence identity to A. gambiae with above 75% identity at the amino acid level. The exception is CYP9J14. The A. gambiae P450 showing highest identity to CYP9J14 exhibits only 55% identity suggesting that CYP9J14 may have arisen from a recent duplication event. Molecular phylogenetic analysis based on amino acid sequences also supported this hypothesis. Intron positions, but not size, were highly conserved between the two species. The high level of orthology that exists in the P450 gene families of these two species may facilitate the prediction of individual P450 protein function. 相似文献
120.
Julie A. Coetzee Marcus J. Byrne & Martin P. Hill 《Entomologia Experimentalis et Applicata》2007,125(3):237-247
Water hyacinth [Eichhornia crassipes (Mart.) Solms (Pontederiaceae)] is the most damaging aquatic weed in South Africa, where five arthropod biological control agents have been released against it. The most recent introduction of Eccritotarsus catarinensis (Carvalho) (Heteroptera: Miridae) has failed to establish permanent populations at a number of sites in South Africa where water hyacinth is a problem. Cold winter temperatures at these sites are assumed to be the reason for these establishment failures. This assumption was tested by investigating the thermal physiology of the mirid, then incorporating these data into various predictive distribution models. Degree‐day models predict 3–14 generations per year at different localities in South Africa, and five generations at a Johannesburg site where the mirid failed to overwinter. The inability to develop sufficiently rapidly during winter months may hinder overwintering of this insect, which was predicted to develop through only one generation during the winter months of April to August in Johannesburg. A CLIMEX model also showed that cold stress limits the mirid's ability to overwinter in the interior of the country, while determination of the lower lethal limit (–3.5 °C) and critical thermal minimum (1.2 ± 1.17 °C) also indicated that extreme temperatures will limit establishment at certain sites. It is concluded that E. catarinensis is limited in its distribution in South Africa by low winter temperatures. 相似文献