Chemisch-entwicklungsphysiologische Beziehungen von Uterus zu Blastocyste des KaninchensOryctolagus cuniculus

Ohne ZusammenfassungFür Überlassung des Themas und für zahlreiche Anregungen danke ich Herrn Prof. Dr. F.Seidel, Direktor des Zoologischen Instituts der Universität Marburg.     

Non-condensation of one segment of a chromosome No. 2 in a male with an otherwise normal karyotype (and severe hypospadias)

Summary A child with severe hypospadias is presented, whose karyotype showed in about 11% of mitoses of peripheral blood one member of chromosome pair No. 2 with a non-condensed region near the centromere. The non-condensed segment does not show late replication, however, it is situated very close to the late replicating segment of the long arms of chromosome No. 2. The nature and possible implications of this kind of aberration are discussed. It is held that non-condensation can produce localized chromosome breaks by a mechanism possibly different from any of the classical breakage mechanisms.     

Reconstitution of the Anti-Apoptotic Bcl-2 Protein into Lipid Membranes and Biophysical Evidence for Its Detergent-Driven Association with the Pro-Apoptotic Bax Protein

The anti-apoptotic B-cell CLL/lymphoma-2 (Bcl-2) protein and its counterpart, the pro-apoptotic Bcl-2-associated X protein (Bax), are key players in the regulation of the mitochondrial pathway of apoptosis. However, how they interact at the mitochondrial outer membrane (MOM) and there determine whether the cell will live or be sentenced to death remains unknown. Competing models have been presented that describe how Bcl-2 inhibits the cell-killing activity of Bax, which is common in treatment-resistant tumors where Bcl-2 is overexpressed. Some studies suggest that Bcl-2 binds directly to and sequesters Bax, while others suggest an indirect process whereby Bcl-2 blocks BH3-only proteins and prevents them from activating Bax. Here we present the results of a biophysical study in which we investigated the putative interaction of solubilized full-length human Bcl-2 with Bax and the scope for incorporating the former into a native-like lipid environment. Far-UV circular dichroism (CD) spectroscopy was used to detect direct Bcl-2-Bax-interactions in the presence of polyoxyethylene-(23)-lauryl-ether (Brij-35) detergent at a level below its critical micelle concentration (CMC). Additional surface plasmon resonance (SPR) measurements confirmed this observation and revealed a high affinity between the Bax and Bcl-2 proteins. Upon formation of this protein-protein complex, Bax also prevented the binding of antimycin A₂ (a known inhibitory ligand of Bcl-2) to the Bcl-2 protein, as fluorescence spectroscopy experiments showed. In addition, Bcl-2 was able to form mixed micelles with Triton X-100 solubilized neutral phospholipids in the presence of high concentrations of Brij-35 (above its CMC). Following detergent removal, the integral membrane protein was found to have been fully reconstituted into a native-like membrane environment, as confirmed by ultracentrifugation and subsequent SDS-PAGE experiments.     

Über die mathematische Behandlung physiologischer Diffusionsprozesse in zylinderförmigen Objekten

Summary Following A. V.Hill, those formulae are developed in a mathematical research which are of interest in the field of physiological diffusion processes in cylindrical objects.At first, an introduction into the problem is given. Then a development follows of the mathematical methods (Bernoulli's method [Produktansatz],Laplace-transformation) which are suitable for the integration of the partial differential equation of the diffusion.The special part contains the investigation of problems which are importance for the physiology of respiration. Thus the query is answered for the concentration of a substance in dependance of time and place for the diffusion in tissue cylinders taking into consideration a constant rate of consumption, as well as the query of the temporal alteration of the average concentration. The cylinder fed from the outside and the one fed from a central capillary (Krogh's) must hereby be specially treated according to the boundary conditions. The developed equations are explained by special examples and numerical lists. Further, the steady state of supply of tissue cylinders is investigated in case that apart from the radial also an axial diffusion component (Längsdiffusion) has to be counted with. The numerical results from the developed formulae are in good agreement with the values found by experiments.

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Résumé Suivant A. V.Hill on a développé dans une étude mathématique les formules qui se rapportent aux procédés de diffusion dans la physiologie.Pour l'introduction, on donne une explication des problèmes. Puis on fait suivre le développement mathématique (méthode de l'intégration deBernoulli, transformationLaplace) qui permettent l'intégration de l'équation différentielle de la diffusion.Dans la partie spéciale, on étudie des problèmes concrets de la physiologie respiratoire. Pour la diffusion dans des cylindres cellulaires on a trouvé le résultat de la concentration d'une substance en dépendance du lieu et du temps où on a tenu compte à une consommation constante. De même, on a réussi a résoudre le problème du changement temporel de la concentration moyenne. Il est nécessaire de traiter en particulier le cylindre dans des conditions spéciales c'est-à-dire quand il est approvisionné du dehors et d'un tube capillaire et central (cylindreKrogh). On a expliqué les équations différentielles par des cas spéciaux et par des tableaux numériques. Puis on a étudié la provisionnement stationnaire des cylindres cellulaires en cas que outre la composante de diffusion radiale, il faut tenir compte à une composante de diffusion axiale (Längsdiffusion). Les résultats obtenus par les formules développées correspondent bien aux valeurs trouvées par les expériences.

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Mit Unterstützung durch Mittel der Deutschen Forschungsgemeinschaft.     

毛离褶伞——一种孢子杆状的离褶伞科新属和新种

根据形态和分子系统发育证据,描述采自我国西南的新属和新种毛离褶伞Tricholyophyllum brunneum。与离褶伞科其他属不同,该新属的菌盖表皮为毛皮状,菌柄表皮为不连续的毛皮状,有褶缘囊状体,担孢子长椭圆形至圆柱形。