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排序方式: 共有394条查询结果,搜索用时 15 毫秒
151.
152.
153.
Analysis of clones carrying repeated DNA sequences in two YAC libraries of Arabidopsis thaliana DNA 总被引:1,自引:0,他引:1
Renate Schmidt Joanna Putterill Joanne West Gerda Cnops Frances Robson George Coupland Caroline Dean 《The Plant journal : for cell and molecular biology》1994,5(5):735-744
YAC clones carrying repeated DNA sequences from the Arabidopsis thaliana genome have been characterized in two widely used Arabidopsis YAC libraries, the EG library and the EW library. Ribosomal, chloroplast and the paracentromeric repeat sequences are differentially represented in the two libraries. The coordinates of YAC clones hybridizing to these sequences are given. A high proportion of EG YAC clones were classified as containing chimaeric inserts because individual clones carried unique sequences and repetitive sequences originating from different locations in the genome. None of the EW YAC clones analysed were chimaeric in this way. YAC clones carrying tandemly repeated sequences, such as the paracentromeric or rDNA sequences, exhibited a high degree of instability. These observations need to be taken into account when using these libraries in the development of a physical map of the Arabidopsis genome and in chromosome walking experiments. 相似文献
154.
The influence on microtubule assembly in vitro of monoclonal antibodies against microtubule-associated proteins (MAPs) was studied. Light scattering was used for measuring net polymer formation and electron microscopy for determining the influence of antibodies on microtubule morphology. Control experiments showed that nonimmune mouse IgG had no effect on either the assembly or appearance of microtubules. The same was true for monoclonal antibodies against MAP1. At low levels, antibodies against MAP2 caused the aggregation of microtubules into bundles, an effect that did not occur with antibodies against any other MAP type studied. At increasing concentrations, anti-MAP2 progressively inhibited tubulin polymerization, producing irregular, shortened filaments. Anti-MAP5 produced a striking fragmentation of microtubules into very short pieces that were otherwise morphologically identical to control microtubules. The different effects of these antibodies show the potential of monoclonal antibodies for investigating MAP function and form an important adjunct to cellular microinjection experiments. 相似文献
155.
Gerda Breitenreuter Marina Lotti Marina Stöffler-Meilicke Georg Stöffler 《Molecular & general genetics : MGG》1984,197(2):189-195
Summary Mice were immunised with 30S subunits from E. coli and their spleen cells were fused with myeloma cells. From this fusion two monoclonal antibodies were obtained, one of which was shown to be specific for ribosomal protein S3, the other for ribosomal protein S7. The two monoclonal antibodies formed stable complexes with intact 30S subunits and were therefore used for the three-dimensional localisation of ribosomal proteins S3 and S7 on the surface of the E. coli small subunit by immuno electron microscopy. The antibody binding sites determined with the two monoclonal antibodies were found to lie in the same area as those obtained with conventional antibodies. Both proteins S3 and S7 are located on the head of the 30S subunit, close to the one-third/two-thirds partition. Protein S3 is located just above the small lobe, whereas protein S7 is located on the side of the large lobe. 相似文献
156.
Gerda Fritsche Reinhold von Sengbusch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1961,31(5):233-238
Ohne ZusammenfassungMit 4 AbbildungenFrau Prof. Dr.E. Schiemann zum 80. Geburtstag gewidmet. 相似文献
157.
Some observations on the use of cylinders made from different materials in the cylinder plate method
Summary Aluminium has been reported as material for test cylinders. Our observations indicate that certain qualities of commercial aluminium tubes exert a pronounced inhibitive effect on a Staph. aureus culture. 相似文献
158.
Gerda Fritsche Reinhold v. Sengbusch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1963,33(7):270-274
Ohne ZusammenfassungMit 9 Abbildungen 相似文献
159.
Emílie Lukášová Stanislav Kozubek Michal Kozubek Jana Kjeronská Leonard Rýznar Jana Horáková Eva Krahulcová Gerda Horneck 《Human genetics》1997,100(5-6):525-535
Quantitative measurements of the nuclear localisation of the ABL and BCR genes and the distance between them were performed
in randomly oriented bone marrow cells of control donors and patients with chronic myeloid leukaemia (CML). Most ABL and BCR
genes (75%) are located at a distance of 20–65% of the local radius from the nuclear centre to the nuclear membrane. A chimeric
BCR-ABL gene located on a derivative chromosome 22 resulting from t(9;22)(q34;q11) [the so-called Philadelphia (Ph) chromosome]
as well as the intact ABL and BCR genes of patients suffering from chronic myeloid leukaemia are also located mostly in this
region, which has a mean thickness of 2 μm in bone marrow cells. We have not found any significant differences in the location
of the two genes in the G1 and G2 phases of the cell cycle, nor between bone marrow cells and stimulated lymphocytes. Irradiation of lymphocytes with a dose
of 5 Gy of γ-rays results in a shift of both genes to the central region of the nucleus (0–20% of the radius distant from
the nuclear centre) in about 15% of the cells. The minimum distance between one ABL and one BCR gene is less than 1 μm in
47.5% of bone marrow cells of control donors. Such a small distance is found between homologous ABL and between homologous
BCR genes in only 8.1% and 8.4% of cells, respectively. It is possible that the relative closeness of nonhomologous ABL and
BCR genes in interphase nuclei of bone marrow cells could facilitate translocation between these genes. In 16.4% of bone marrow
cells one ABL and one BCR gene are juxtaposed (the distance between them varies from 0–0.5 μm) and simulate the Ph chromosome.
This juxtaposition is the result of the projection of two genes located one above another into a plane, as follows from the
probability calculation.
Received: 5 September 1996 / Accepted: 15 April 1997 相似文献
160.
Gerda J. van der Brugge-Gamelkoorn Marja B. van de Ende T. Sminia 《Cell and tissue research》1985,239(1):177-182
Immunohistochemical, enzyme-histochemical and electron-microscopical methods were used to study non-lymphoid cells of control and stimulated rat bronchus associated lymphoid tissue (BALT) in situ and in suspensions. Particular attention was paid to the so-called antigen-handling cells, i.e., the interdigitating cells (IDC), which are situated in the T-cell areas, the follicular dendritic cells (FDC), which appear to be restricted to germinal centers, and macrophages, present both in T-cell and B-cell areas. The interdigitating cells were distinguished by being Ia-positive and by the presence of acid phosphatase and non-specific esterase activity in an area near the nucleus. Follicular dendritic cells could be observed in situ by using a monoclonal antibody and by the in vitro trapping of HRP-anti-HRP complexes. Several types of macrophages were found. At the electron-microscopical level no well-developed IDC and FDC could be detected in control BALT. However, in BALT of lipopolysaccharide-stimulated and mycoplasma-infected rats, well-developed IDC and FDC were found. It can be concluded that IDC's and FDC's can be found in BALT. 相似文献