全文获取类型
收费全文 | 359篇 |
免费 | 10篇 |
出版年
2023年 | 2篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 15篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2013年 | 8篇 |
2012年 | 15篇 |
2011年 | 16篇 |
2010年 | 15篇 |
2009年 | 15篇 |
2008年 | 21篇 |
2007年 | 13篇 |
2006年 | 13篇 |
2005年 | 17篇 |
2004年 | 14篇 |
2003年 | 12篇 |
2002年 | 9篇 |
2001年 | 18篇 |
2000年 | 8篇 |
1999年 | 11篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1995年 | 2篇 |
1992年 | 7篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 6篇 |
1972年 | 3篇 |
1971年 | 5篇 |
1970年 | 5篇 |
1967年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有369条查询结果,搜索用时 15 毫秒
11.
V. A. Gvozdev T. I. Gerasimova G. L. Kogan J. M. Rosovsky 《Molecular & general genetics : MGG》1977,153(2):191-198
Summary The molecular nature of lethal and semilethal mutations in the Pgd locus of D. melanogaster coding for 6-phosphogluconate dehydrogenase (6PGD) was studied. All the 11 mutations affect the structural gene of the Pgd locus: 3 semilethal mutations resulted in altered 6PGD molecules with decreased catalytic activities; the rest 8 lethals were null alleles characterized by mutant polypeptides capable of reacting with antisera against highly purified 6PGD.Null or low activity alleles for glucose-6-phosphate dehydrogenase induced by ethyl methanesulfonate were shown to be suppressors for the lethal mutations in the Pgd locus.A monocistronic type of organization of the Pgd locus is suggested taking into account the biochemical mechanism of suppression of the Pgd-lethals and their location in the structural gene coding for 6PGD. 相似文献
12.
Dominant Effects of Suppressor of Hairy-Wing Mutations on Gypsy-Induced Alleles of Forked and Cut in Drosophila Melanogaster
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mutations induced by the gypsy retrotransposon in the forked (f) and cut (ct) loci render their expression under the control of the suppressor of Hairy-wing [su(Hw)] gene. This action is usually recessive, but su(Hw) acts as a dominant on the alleles fk, ctk and ctMRpN30. Molecular analysis of the gypsy element present in fk indicates that this allele is caused by the insertion of a modified gypsy in which the region normally containing twelve copies of the octamer-like repeat that interacts with the su(Hw) product is altered. Analysis of the gypsy element responsible for the ctk and ctMRpN30 mutations also reveals a correlation between the dominant action of su(Hw) and disruption of the octamer region. We propose that these disruptions alter the affinity and interaction of su(Hw) protein with gypsy DNA, thereby sensitizing the mutant phenotype to fluctuations in su(Hw) product. 相似文献
13.
Plasmid DNA transduction with mini-D3112 delta H, deletion derivative of phage D3112, which lost the genes essential for phage growth but retained the sites required for transposition and packaging was studied. Unlike D3112, mini-D3112 delta H element can transduce plasmids and plasmid markers at frequencies of 10(-5)-10(-8) in rec+ cells of Pseudomonas aeruginosa. Plasmids R1162 and R388 of the size smaller than phage genome were transduced intact. Large plasmids, like RP4 and R151, were deleted under transduction. By this way, we isolated deletion derivatives of RP4. The smallest derivative pN2 contained a 4.5 kb fragment of RP4. Unlike the latter, pN2 plasmid had narrow host range and did not maintain in Escherichia coli cells. 相似文献
14.
15.
Taratuhin O. D. Novikova L. Yu. Seferova I. V. Gerasimova T. V. Nuzhdin S. V. Samsonova M. G. Kozlov K. N. 《Biophysics》2020,65(1):106-117
Biophysics - Abstract—Soybean phenology is strongly influenced by temperature and day length, and phenological records clearly reflect the changes in climatic conditions. A model including... 相似文献
16.
Evan M. Cornett Yulia V. Gerasimova Dmitry M. Kolpashchikov 《Bioorganic & medicinal chemistry》2013,21(7):1988-1991
Inhibitors that covalently damage proteins or nucleic acids offer great potency, but are difficult to rationally design and suffer from poor specificity. Here we outline a general concept for constructing covalent inhibitors, called the two-component covalent inhibitor (TCCI). The approach takes advantage of two ligand analogs equipped with pre-reactive groups. Binding of the analogs to the adjacent sites of a target biopolymer brings the pre-reactive groups in close proximity and causes their interaction followed by covalent damage of the target. In the present study we used light-activated pre-reactive groups to inactivate a DNA polymerase. It was found that the efficiency of a traditional single-component inhibitor was greatly reduced in the presence of a non-target protein, while the TCCI was not significantly affected. Our findings suggest that TCCI approach has advantages in inactivation of biopolymers in complex multi-component systems. 相似文献
17.
Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton's jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium. 相似文献
18.
Oral pathogens have created a menace in recent years due to biofilm formation and antimicrobial drug resistance. The current treatment strategy works well with antibiotics. However, constant use of antibiotics creates a selective pressure, which increases adaptability of the pathogens. Therefore, it is of interest to analyze the potential targets of genistein in dental pathogens using computer aided prediction tools. 相似文献
19.
20.