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31.
Repair of UV damage in plasmid DNA by human fibroblasts 总被引:1,自引:0,他引:1
Hans Mooibroek Bauke de Jong Gerard Venema 《Molecular & general genetics : MGG》1984,195(1-2):175-179
Summary Plasmid DNA from Bacillus subtilis was introduced into monolayers of human fibroblasts by means of a modification of the calcium phosphate coprecipitation technique, comprising centrifugation of the coprecipitate onto the cells and treatment with polyethyleneglycol. The amount of DNA resistant to removal from the monolayers ranged from 10% to 15% of the input DNA. By determination of the biological activity of the plasmid DNA, re-extracted after various periods following entry into the fibroblasts and subsequently used as donor for B. subtilis protoplasts, it was shown that the activity of the plasmid DNA was gradually lost. When ultraviolet light-inactivated plasmid DNA was used as donor, reactivation of the plasmid was observed, which was completed within 2 h. The dose-dependent incorporation of [14C]-thymidine suggests that DNA repair processes were involved in reactivation of the plasmid DNA. 相似文献
32.
Dr. Paul A. Lespinat Yves M. Berlier Guy D. Fauque Rene Toci Gerard Denariaz Jean LeGall 《Journal of industrial microbiology & biotechnology》1987,1(6):383-388
Summary Hydrogenase and nitrogenase activities of sulfate-reducing bacteria allow their adaptation to different nutritional habits even under adverse conditions. These exceptional capabilities of adaptation are important factors in the understanding of their predominant role in problems related to anaerobic metal corrosion. Although the D2–H+ exchange reaction indicated thatDesulfovibrio desulfuricans strain Berre-Sol andDesulfovibrio gigas hydrogenases were reversible, the predominant activity in vivo was hydrogen uptake. Hydrogen production was restricted to some particular conditions such as sulfate or nitrogen starvation. Under diazotrophic conditions, a transient hydrogen evolution was followed by uptake when dinitrogen was effectively fixed. In contrast, hydrogen evolution proceeded when acetylene was substituted as the nitrogenase substrate. Hydrogen can thus serve as an electron donor in sulfate reduction and nitrogen metabolism. 相似文献
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Richard F. Selden Andre Steinmetz Lee McIntosh Lawrence Bogorad Gerard Burkard Mfika Mubumbila Marcel Kuntz Edwin J. Crouse Jacques H. Weil 《Plant molecular biology》1983,2(3):141-153
A minimum of 37 genes corresponding to tRNAs for 17 different amino acids have been localized on the restriction endonuclease cleavage site map of theZea mays chloroplast DNA molecule. Of these, 14 genes corresponding to tRNAs for 11 amino acids are located in the larger of the two single-copy regions which separate the two inverted copies of the repeat region. One tRNA gene is in the smaller single-copy region. Each copy of the large repeated sequence contains, in addition to the ribosomal RNA genes, 11 tRNA genes corresponding to tRNAs for 8 amino acids. The genes for tRNA2 Ile and tRNAAla map in the ribosomal spacer sequence separating the 16S and 23S ribosomal RNA genes. The three isoaccepting species for the tRNAsLeu and the three for tRNAsSer, as well as the two isoaccepting species for tRNAAsn, tRNAGly, tRNAsIle, tRNAsMet, tRNAsThr, are shown to be encoded at different loci. Two independent methods have been used for the localization of tRNA genes on the physical map of the maize chloroplast DNA molecule: (a) cloned chloroplast DNA fragments were hybridized with radioactively-labelled total 4S RNAs, the hybridized RNAs were then eluted, and identified by two-dimensional polyacrylamide gel electrophoresis, and (b) individual tRNAs were32P-labelledin vitro and hybridized to DNA fragments generated by digestion of maize chloroplast DNA with various restriction endonucleases. 相似文献
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37.
ELECTRON MICROSCOPIC OBSERVATIONS OF RAT AND MOUSE CEREBELLUM IN TISSUE CULTURE 总被引:12,自引:8,他引:4
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Closely ordered stages of myelin formation in cultures of newborn rat and mouse cerebellum, selected by direct light microscopy, were studied with the electron microscope. Electron micrographs of these cultures reveal the presence of neurons, axons, neuroglia, microglia, and ependymal cells. The appearance of the neuron is identical to that previously described in vivo. The neuroglial cell has long, branching processes, and its cytoplasm is characterized by packets of long, narrow fibrils. During myelin formation, a glial cell process surrounds the axon. This process may form an internal mesaxon and may spiral for several turns around the axon. Other glial cell processes may interdigitate with or overlay the innermost process to contribute to the multilamellated structure. The glial processes flatten and the cytoplasmic surfaces of the cell membrane come into contact to form the lamellae of the myelin sheath. These adhesions may be temporarily incomplete as evidenced by sequestered islands of glial cytoplasm among the myelin lamellae. Ultimately, a compact, apparently spiral, myelin sheath is formed. These findings are discussed in relation to in vivo central myelin formation. 相似文献
38.
A biometeorological analysis of the daily asthma complaints, classified in 5 degrees of severity, of 50 boys and 25 girls, 7 to 16 years old, at the asthma center Eykeloord (Eastern Netherlands), during 1964 has shown that in winter and early spring the number of asthma attacks in both sexes increased during periods of cooling. The total rise in asthma attacks increased with the length of the cooling period and decreased in relation to the length of the period of warming up. During the summer months June and August these meteorotropic effects were not statistically significant. The observations confirmed the previous findings at an asthma center in the Western Netherlands.
Zusammenfassung Eine biometeorologische Analyse der täglichen Asthmabeschwerden, die in 5 Schweregraden klassifiziert wurden, bei 50 Knaben und 25 Mädchen im Alter von 7 bis 16 Jahren in der Asthmaklinik Eykeloord (Ost-Holland)während des Jahres 1964 hat gezeigt, dass im Winter und Vorfrühling die Anzahl der Asthmaanfälle bei Kälteeinbrüchen zunimmt. Asthmaanfälle nehmen an Zahl mit der Länge der Kälteperioden zu und mit der Länge der Wärmeperioden ab.Während der Sommermonate Juni bis August waren diese Beziehungen zwar auch nachweisbar aber nicht signifikant. Die Beobachtungen bestätigten früheren Ergebnisse in einer Asthmaklinik in West-Holland.
Resume Une analyse biométéorologique des accès d'asthme a montré qu'en hiver et au début du printemps le nombre des dits accès augmente au moment d'une advection d'air froid. Pour ce faire, on s'est basé sur l'observation de 50 garçons et de 25 fillettes de 7 à 16 ans répartis en 5 classes d'acuité de la maladie. Tous étaient en traitement durant l'année 1964 à la clinique pour asthmatiques d'Eykeloord(Hollande orientale). Les accès d'asthme sont d'autant plus nombreux que la période froide est plus longue et leur nombre diminue parallèlement à la durée des périodes plus chaudes. La même relation a pu être établie durant les mois d'été (de juin à août), mais pas de façon significative. Ces observations confirment celles qui avaient été faites antérieurement dans une clinique pour asthmatiques de l'ouest de la Hollande.相似文献
39.
Natalia G. Stoicheva Christopher L. Davey Gerard H. Markx Douglas B. Kell 《Biocatalysis and Biotransformation》1989,2(4):245-255
Dielectric spectroscopy provides a convenient means of determining the degree of intactness of biological cells. 4-terminal dielectric measurements of suspensions of Saccharomyces cerevisiae at 0.4 MHz show that, as with all other biological cells, these organisms possess a substantial β-dispersion. The additional of octanol to such suspensions causes a rapid decrease in the electrical capacitance of the suspension, which parallels the cellular viability as determined by methylene blue staining. The kinetics of cell death are determined in part by the rate of dissolution of the organic solvent in the aqueous phase. The toxicity of several organic solvents to S. cerevisiae is studied using this technique, and is found to be dependent upon the polarity of the solvent. The present method provides a simple and rapid means for assessing the biocompatibility of solvents used in biotransformations. 相似文献
40.
Study of the in vitro bioactivation of albendazole in human liver microsomes and hepatoma cell lines
Sylvie Rolin Hajar Souhaili-El Amri Anne-Marie Batt Michele Levy Denyse Bagrel Gerard Siest 《Cell biology and toxicology》1989,5(1):1-14
The metabolism of albendazole (ABZ), a benzimidazole anthelminthic, was studied in either microsomal preparations of human liver biopsies or cultured human hepatoma cell lines. Metabolites were analyzed by HPLC. Our data show that microsomes from human biopsies and two human cell lines, HepG2 and Hep3B, oxidize the drug to the sulfoxide very efficiently, whereas the third cell line tested, SK-HEP-1, does not. Both cytochrome P-450 dependent monooxygenases and favin-containing monooxygenases appear to be involved in human ABZ metabolism. Using the cell line displaying the highest ABZ-metabolizing activity, HepG2, the cytotoxic and the inducing effects of the parent drug ABZ and of two primary metabolites, the sulfoxide and the sulfone were studied. These three chemicals provoked a rise in mitotic index resulting from cell division blockage at the prophase or at the metaphase (ABZ metabolites) stage, and ABZ was more cytotoxic than its metabolites. With regard to enzyme-inducing effects, our data clearly demonstrate that the sulfoxide and, to a lesser degree, the sulfone are potent inducers of some drug metabolizing enzymes (i.e., cytochrome P-488 dependent monooxygenases and UDP glucuronyltransferase), whereas ABZ fails to increase and even slightly decreases these enzymatic activities. In conclusion, the HepG2 human hepatoma cell line appears to be suitable for the study of many parameters of metabolism and action of ABZ and other structurally related compounds in humans.Abbreviations ABZ
albendazole
- B[a]P
benzo[a]pyrene
- HPLC
high-performance liquid chromatography
- MC
3-methylcholanthrene
- MFO
mixed-function oxidase
- UDPGT
UDP-glucuronyltransferase 相似文献