全文获取类型
收费全文 | 8300篇 |
免费 | 1238篇 |
国内免费 | 2篇 |
专业分类
9540篇 |
出版年
2021年 | 83篇 |
2019年 | 70篇 |
2018年 | 92篇 |
2017年 | 66篇 |
2016年 | 134篇 |
2015年 | 219篇 |
2014年 | 266篇 |
2013年 | 361篇 |
2012年 | 420篇 |
2011年 | 442篇 |
2010年 | 281篇 |
2009年 | 244篇 |
2008年 | 378篇 |
2007年 | 455篇 |
2006年 | 451篇 |
2005年 | 480篇 |
2004年 | 469篇 |
2003年 | 455篇 |
2002年 | 419篇 |
2001年 | 196篇 |
2000年 | 111篇 |
1999年 | 146篇 |
1998年 | 111篇 |
1997年 | 96篇 |
1996年 | 93篇 |
1995年 | 89篇 |
1994年 | 85篇 |
1993年 | 77篇 |
1992年 | 108篇 |
1991年 | 96篇 |
1990年 | 92篇 |
1989年 | 81篇 |
1988年 | 95篇 |
1987年 | 98篇 |
1986年 | 100篇 |
1985年 | 106篇 |
1984年 | 110篇 |
1983年 | 89篇 |
1982年 | 95篇 |
1981年 | 125篇 |
1980年 | 109篇 |
1979年 | 96篇 |
1978年 | 81篇 |
1977年 | 97篇 |
1976年 | 85篇 |
1975年 | 81篇 |
1974年 | 92篇 |
1973年 | 78篇 |
1971年 | 67篇 |
1970年 | 67篇 |
排序方式: 共有9540条查询结果,搜索用时 15 毫秒
991.
Genetic map-based analysis of genome structure in the homosporous fern Ceratopteris richardii 下载免费PDF全文
Homosporous ferns have extremely high chromosome numbers relative to flowering plants, but the species with the lowest chromosome numbers show gene expression patterns typical of diploid organisms, suggesting that they may be diploidized ancient polyploids. To investigate the role of polyploidy in fern genome evolution, and to provide permanent genetic resources for this neglected group, we constructed a high-resolution genetic linkage map of the homosporous fern model species, Ceratopteris richardii (n = 39). Linkage map construction employed 488 doubled haploid lines (DHLs) that were genotyped for 368 RFLP, 358 AFLP, and 3 isozyme markers. Forty-one linkage groups were recovered, with average spacing between markers of 3.18 cM. Most loci (approximately 76%) are duplicated and most duplicates occur on different linkage groups, indicating that as in other eukaryotic genomes, gene duplication plays a prominent role in shaping the architecture of fern genomes. Although past polyploidization is a potential mechanism for the observed abundance of gene duplicates, a wide range in the number of gene duplicates as well as the absence of large syntenic regions consisting of duplicated gene copies implies that small-scale duplications may be the primary mode of gene duplication in C. richardii. Alternatively, evidence of past polyploidization(s) may be masked by extensive chromosomal rearrangements as well as smaller-scale duplications and deletions following polyploidization(s). 相似文献
992.
Manen JF Habashi C Jeanmonod D Park JM Schneeweiss GM 《Molecular phylogenetics and evolution》2004,33(2):482-500
The rbcL sequences of 106 specimens representing 28 species of the four recognized sections of Orobanche were analyzed and compared. Most sequences represent pseudogenes with premature stop codons. This study confirms that the American lineage (sects. Gymnocaulis and Myzorrhiza) contains potentially functional rbcL-copies with intact open reading frames and low rates of non-synonymous substitutions. For the first time, this is also shown for a member of the Eurasian lineage, O. coerulescens of sect. Orobanche, while all other investigated species of sects. Orobanche and Trionychon contain pseudogenes with distorted reading frames and significantly higher rates of non-synonymous substitutions. Phylogenetic analyses of the rbcL sequences give equivocal results concerning the monophyly of Orobanche, and the American lineage might be more closely related to Boschniakia and Cistanche than to the other sections of Orobanche. Additionally, species of sect. Trionychon phylogenetically nest in sect. Orobanche. This is in concordance with results from other plastid markers (rps2 and matK), but in disagreement with other molecular (nuclear ITS), morphological, and karyological data. This might indicate that the ancestor of sect. Trionychon has captured the plastid genome, or parts of it, of a member of sect. Orobanche. Apart from the phylogenetically problematic position of sect. Trionychon, the phylogenetic relationships within sect. Orobanche are similar to those inferred from nuclear ITS data and are close to the traditional groupings traditionally recognized based on morphology. The intraspecific variation of rbcL is low and is neither correlated with intraspecific morphological variability nor with host range. Ancestral character reconstruction using parsimony suggests that the ancestor of O. sect. Orobanche had a narrow host range. 相似文献
993.
994.
Aagaard A Listwan P Cowieson N Huber T Ravasi T Wells CA Flanagan JU Kellie S Hume DA Kobe B Martin JL 《Structure (London, England : 1993)》2005,13(2):309-317
Latexin, the only known mammalian carboxypeptidase inhibitor, has no detectable sequence similarity with plant and parasite inhibitors, but it is related to a human putative tumor suppressor protein, TIG1. Latexin is expressed in the developing brain, and we find that it plays a role in inflammation, as it is expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets. The crystal structure of mouse latexin, solved at 1.83 A resolution, shows no structural relationship with other carboxypeptidase inhibitors. Furthermore, despite a lack of detectable sequence duplication, the structure incorporates two topologically analogous domains related by pseudo two-fold symmetry. Surprisingly, these domains share a cystatin fold architecture found in proteins that inhibit cysteine proteases, suggesting an evolutionary and possibly functional relationship. The structure of the tumor suppressor protein TIG1 was modeled, revealing its putative membrane binding surface. 相似文献
995.
996.
Steinauer ML Hanelt B Mwangi IN Maina GM Agola LE Kinuthia JM Mutuku MW Mungai BN Wilson WD Mkoji GM Loker ES 《Molecular ecology》2008,17(23):5062-5074
Hybridization and introgression can have important consequences for the evolution, ecology and epidemiology of pathogenic organisms. We examined the dynamics of hybridization between a trematode parasite of humans, Schistosoma mansoni, and its sister species, S. rodhaini, a rodent parasite, in a natural hybrid zone in western Kenya. Using microsatellite markers, rDNA and mtDNA, we showed that hybrids between the two species occur in nature, are fertile and produce viable offspring through backcrosses with S. mansoni. Averaged across collection sites, individuals of hybrid ancestry comprised 7.2% of all schistosomes collected, which is a large proportion given that one of the parental species, S. rodhaini, comprised only 9.1% of the specimens. No F1 individuals were collected and all hybrids represented backcrosses with S. mansoni that were of the first or successive generations. The direction of introgression appears highly asymmetric, causing unidirectional gene flow from the rodent parasite, S. rodhaini, to the human parasite, S. mansoni. Hybrid occurrence was seasonal and most hybrids were collected during the month of September over a 2-year period, a time when S. rodhaini was also abundant. We also examined the sex ratios and phenotypic differences between the hybrids and parental species, including the number of infective stages produced in the snail host and the time of day the infective stages emerge. No statistical differences were found in any of these characteristics, and most of the hybrids showed an emergence pattern similar to that of S. mansoni. One individual, however, showed a bimodal emergence pattern that was characteristic of both parental species. In conclusion, these species maintain their identity despite hybridization, although introgression may cause important alterations of the biology and epidemiology of schistosomiasis in this region. 相似文献
997.
T. Spencer Wells 《BMJ (Clinical research ed.)》1890,2(1565):1465-1468
998.
T. Spencer Wells 《BMJ (Clinical research ed.)》1889,1(1484):1280-1281
999.
1000.