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651.
652.
Porges Karl Stewart Ian G. Hoßfeld Uwe Levit Georgy S. 《Russian Journal of Developmental Biology》2019,50(6):290-302
Russian Journal of Developmental Biology - Since Charles Darwin (1809–1882) and Ernst Haeckel (1834–1919) published their trailblazing ideas, the scientific community’s discussion... 相似文献
653.
Mikhail O. Maksimov Joseph D. Koos Chuhan Zong Bozhena Lisko A. James Link 《The Journal of biological chemistry》2015,290(52):30806-30812
Lasso peptide isopeptidase is an enzyme that specifically hydrolyzes the isopeptide bond of lasso peptides, rendering these peptides linear. To carry out a detailed structure-activity analysis of the lasso peptide isopeptidase AtxE2 from Asticcacaulis excentricus, we solved NMR structures of its substrates astexin-2 and astexin-3. Using in vitro enzyme assays, we show that the C-terminal tail portion of these peptides is dispensable with regards to isopeptidase activity. A collection of astexin-2 and astexin-3 variants with alanine substitutions at each position within the ring and the loop was constructed, and we showed that all of these peptides except for one were cleaved by the isopeptidase. Thus, much like the lasso peptide biosynthetic enzymes, lasso peptide isopeptidase has broad substrate specificity. Quantitative analysis of the cleavage reactions indicated that alanine substitutions in loop positions of these peptides led to reduced cleavage, suggesting that the loop is serving as a recognition element for the isopeptidase. 相似文献
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655.
Cellobiose may exert different effects on the activities of various endoglucanases. The endoglucanases of T. reesei and Rapidase are noticeably suppressed by cellobiose at concentrations above 3 mM. On the other hand, a low molecular weight endoglucanase from T. koningii is activated by cellobiose, whereas high molecular weight endoglucanases from the same source are inhibited by cellobiose. A detailed kinetic analysis of the effects showed that the low molecular weight endoglucanase is activated by a transglycosylation mechanism, in which cellobiose acts as an additional nucleophile. At saturating concentrations of cellobiose (Ks = 15 mM) the enzyme activity is increased 6-fold. Such a specific mechanism of activation manifests itself in an acceleration of random cleavage of CM-cellulose by the low molecular weight endoglucanase, which can be recorded by a viscosimetric technique. However, its action does not accelerate the production of soluble reducing sugars. 相似文献
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