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881.
Highly selective extraction of spiralin from the Spiroplasma citri cell membrane with alkyl-N-sulfobetaines 总被引:2,自引:0,他引:2
The extraction of proteins from the membrane of the mollicute (mycoplasma) Spiroplasma citri by sodium N-dodecyl-N,N-dimethyl-3-amino-1-propane sulfonate (SB12) and sodium N-tetradecyl-N,N-dimethyl-3-amino-1-propane sulfonate (SB14) was studied with electrophoretic methods. The membranes were prepared by osmotic lysis of the cells and depleted of the bulk of extrinsic proteins. It was possible to extract up to 35 and 45% of membrane proteins with SB12 and SB14, respectively. Maximal yield was obtained in both cases with detergent concentrations greater than or equal to 5 mumoles/mg of membrane protein. Spiralin, the major protein in the S. citri membrane, was highly selectively solubilized without the loss of antigenicity, with a yield of about 90% with SB12 and close to 100% with SB14, for a detergent concentration greater than or equal to 0.2 M. The degree of selectivity in favour of spiralin was higher with SB12 (purity approximately equal to 70%) than with SB14 (purity approximately equal to 50%). Treatment of the S. citri membrane with high concentrations of SB12 is a simple and fast procedure for partial purification of spiralin. This example shows that, in some cases, it should be possible to modulate the selectivity of the extraction of membrane proteins simply by varying the relative concentration of detergent. 相似文献
882.
Georges Combaut Jean-Marie Chantraine Jean Teste Karl-W. Glombitza 《Phytochemistry》1978,17(10):1791-1792
Cyclotribromoveratrylene has been isolated from an ethanolic extract of fresh Halopytis pinastroïdes, after treatment with diazomethane. This new 相似文献
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A simple method for measuring stiffness during running 总被引:1,自引:0,他引:1
Morin JB Dalleau G Kyröläinen H Jeannin T Belli A 《Journal of applied biomechanics》2005,21(2):167-180
The spring-mass model, representing a runner as a point mass supported by a single linear leg spring, has been a widely used concept in studies on running and bouncing mechanics. However, the measurement of leg and vertical stiffness has previously required force platforms and high-speed kinematic measurement systems that are costly and difficult to handle in field conditions. We propose a new "sine-wave" method for measuring stiffness during running. Based on the modeling of the force-time curve by a sine function,this method allows leg and vertical stiffness to be estimated from just a few simple mechanical parameters: body mass, forward velocity, leg length, flight time, and contact time. We compared this method to force-platform-derived stiffness measurements for treadmill dynamometer and overground running conditions, at velocities ranging from 3.33 m.s-1 to maximal running velocity in both recreational and highly trained runners. Stiffness values calculated with the proposed method ranged from 0.67 % to 6.93 % less than the force platform method, and thus were judged to be acceptable. Furthermore, significant linear regressions (p < 0.01) close to the identity line were obtained between force platform and sine-wave model values of stiffness. Given the limits inherent in the use of the spring-mass model, it was concluded that this sine-wave method allows leg and stiffness estimates in running on the basis of a few mechanical parameters, and could be useful in further field measurements. 相似文献
886.
Pfaff AW Georges S Abou-Bacar A Letscher-Bru V Klein JP Mousli M Candolfi E 《Immunology and cell biology》2005,83(5):483-489
Materno-foetal transmission causes one of the most serious forms of infection with the intracellular protozoan parasite Toxoplasma gondii. In the placenta, trophoblast cells constitute the barrier between maternal circulation and foetal tissue. We looked at the factors that determine the extent of cell adhesion to human BeWo trophoblast cells during T. gondii infection. BeWo monolayers stimulated with the supernatant of T. gondii-infected PBMC showed a large increase in THP-1 cell adhesion and upregulation of the intercellular adhesion molecule (ICAM)-1. Neutralization of cytokines by corresponding antibodies demonstrated that anti-IFN-gamma, but not anti-TNF-alpha or anti-IL-1beta, led to a significant reduction of THP-1 adhesion to a BeWo monolayer. Treatment of BeWo cells with single cytokines failed to induce upregulation of adhesion. In contrast, simultaneous treatment with IFN-gamma and either TNF-alpha or IL-1beta mimicked strongly the effect of infected cell supernatant. The results suggest that IFN-gamma plays a pivotal role in the cell adhesion process through upregulation of ICAM-1 and in the process of congenital transmission of T. gondii. 相似文献
887.
Identification using phage display of peptides promoting targeting and internalization into HPV-transformed cell lines 总被引:2,自引:0,他引:2
Robinson P Stuber D Deryckère F Tedbury P Lagrange M Orfanoudakis G 《Journal of molecular recognition : JMR》2005,18(2):175-182
'High-risk' human papilloma viruses (HPVs) cause cervical tumours. In order to treat these tumours therapeutic approaches must be developed that efficiently target the tumour cells. Using phage display, we selected tumour-targeting peptides from a library of constrained nonamer peptides presented multivalently on pVIII of M13. Three different consensus peptide sequences were isolated by biopanning on HPV16-transformed SiHa cells. The corresponding phage-peptides targeted and were internalized in HPV16 transformed SiHa and CaSki cells as well as in HPV18-transformed HeLa cells, but failed to bind a panel of normal or transformed cell lines. Two of the three selected peptides targeted cells only when presented on phage particles in a constrained conformation. However, all three peptides retained their targeting capacity when presented on the reporter protein enhanced green fluorescent protein (EGFP) in a monovalent form. These peptides may be useful for the design of drug or gene delivery vectors for the treatment of cervical cancer. 相似文献
888.
Pochron ST Morelli TL Terranova P Scirbona J Cohen J Kunapareddy G Rakotonirina G Ratsimbazafy R Rakotosoa R Wright PC 《American journal of primatology》2005,65(2):103-115
Scent-marking behavior has been well documented in many primate species. Three common functions attributed to scent-marking in males of multi-male/multi-female lemur species include: 1) advertisement of individual identity, 2) territorial defense, and 3) reproductive suppression. We examined the average number of scent-marks per hour exhibited daily by adult male sifakas (Propithecus edwardsi) and found that patterns of scent-marking changed with season, natal status, and dominance status. Males in single-male groups scent-marked at the highest rate, followed by dominant males, males of equal status, and subordinate males. Non-natal males generally scent-marked at higher rates than natal males, and adult males living in a natal group without a parent marked at higher rates than males living with a parent. All males scent-marked at higher rates in the migration season compared to the other seasons. These patterns were consistent with territorial defense and advertisement to females, and the suggestion that these chemical signals impart information concerning status. Since scent-marking behavior tracked seasons and varied with both dominance and natal status, it may serve multiple functions in males. 相似文献
889.
Ganter B Tugendreich S Pearson CI Ayanoglu E Baumhueter S Bostian KA Brady L Browne LJ Calvin JT Day GJ Breckenridge N Dunlea S Eynon BP Furness LM Ferng J Fielden MR Fujimoto SY Gong L Hu C Idury R Judo MS Kolaja KL Lee MD McSorley C Minor JM Nair RV Natsoulis G Nguyen P Nicholson SM Pham H Roter AH Sun D Tan S Thode S Tolley AM Vladimirova A Yang J Zhou Z Jarnagin K 《Journal of biotechnology》2005,119(3):219-244
Successful drug discovery requires accurate decision making in order to advance the best candidates from initial lead identification to final approval. Chemogenomics, the use of genomic tools in pharmacology and toxicology, offers a promising enhancement to traditional methods of target identification/validation, lead identification, efficacy evaluation, and toxicity assessment. To realize the value of chemogenomics information, a contextual database is needed to relate the physiological outcomes induced by diverse compounds to the gene expression patterns measured in the same animals. Massively parallel gene expression characterization coupled with traditional assessments of drug candidates provides additional, important mechanistic information, and therefore a means to increase the accuracy of critical decisions. A large-scale chemogenomics database developed from in vivo treated rats provides the context and supporting data to enhance and accelerate accurate interpretation of mechanisms of toxicity and pharmacology of chemicals and drugs. To date, approximately 600 different compounds, including more than 400 FDA approved drugs, 60 drugs approved in Europe and Japan, 25 withdrawn drugs, and 100 toxicants, have been profiled in up to 7 different tissues of rats (representing over 3200 different drug-dose-time-tissue combinations). Accomplishing this task required evaluating and improving a number of in vivo and microarray protocols, including over 80 rigorous quality control steps. The utility of pairing clinical pathology assessments with gene expression data is illustrated using three anti-neoplastic drugs: carmustine, methotrexate, and thioguanine, which had similar effects on the blood compartment, but diverse effects on hepatotoxicity. We will demonstrate that gene expression events monitored in the liver can be used to predict pathological events occurring in that tissue as well as in hematopoietic tissues. 相似文献
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