全文获取类型
收费全文 | 2035篇 |
免费 | 140篇 |
国内免费 | 1篇 |
专业分类
2176篇 |
出版年
2022年 | 13篇 |
2021年 | 31篇 |
2020年 | 8篇 |
2019年 | 11篇 |
2018年 | 17篇 |
2017年 | 17篇 |
2016年 | 39篇 |
2015年 | 53篇 |
2014年 | 85篇 |
2013年 | 101篇 |
2012年 | 119篇 |
2011年 | 111篇 |
2010年 | 79篇 |
2009年 | 65篇 |
2008年 | 119篇 |
2007年 | 102篇 |
2006年 | 95篇 |
2005年 | 105篇 |
2004年 | 76篇 |
2003年 | 121篇 |
2002年 | 89篇 |
2001年 | 28篇 |
2000年 | 26篇 |
1999年 | 33篇 |
1998年 | 44篇 |
1997年 | 24篇 |
1996年 | 33篇 |
1995年 | 31篇 |
1994年 | 23篇 |
1993年 | 34篇 |
1992年 | 36篇 |
1991年 | 26篇 |
1990年 | 27篇 |
1989年 | 26篇 |
1988年 | 14篇 |
1987年 | 22篇 |
1986年 | 12篇 |
1985年 | 29篇 |
1984年 | 22篇 |
1983年 | 14篇 |
1982年 | 24篇 |
1981年 | 15篇 |
1980年 | 22篇 |
1979年 | 25篇 |
1978年 | 11篇 |
1977年 | 14篇 |
1976年 | 12篇 |
1975年 | 9篇 |
1974年 | 12篇 |
1973年 | 11篇 |
排序方式: 共有2176条查询结果,搜索用时 15 毫秒
41.
42.
Matthew J. Friedman Ante M. Krstulović Henri Colin Georges Guiochon Kathleen Pajer 《Analytical biochemistry》1984,142(2):480-486
Described in this paper is a rapid, isocratic assay for serum indole-3-acetic acid (IAA). The sample preparation involves only protein precipitation using sulfosalicylic acid, and the sensitivity of amperometric detection is in the picogram range. The chromatographic analysis time is approximately 4 min. The devised method was used for a longitudinal study of IAA levels in serum samples from control subjects and newly abstinent alcoholics. Dietary variations were eliminated by administering a 2.0-g loading dose of L-Trp to all subjects investigated. The results are presented in the form of cumulative frequency polygons. Preliminary data indicate no differences in IAA levels between newly abstinent alcoholics and control subjects. 相似文献
43.
Contact of adjacent root hairs of seedlings of white clover ( Trifolium repens L. cv. Ladino and Louisiana Nolin) led to cell-cell adhesion of root hair tips. The involvement of the root lectin, trifoliin A, in this phenomen was examined in slide cultures of axenically grown seedlings. Trifoliin A was detected by indirect immunofluorescence on root hair tips, which had adhered to one another. Seedlings grown under conditions which specifically reduce the levels of this lectin on the root surface (e.g., in the presence of 15 m M NO3 – or 5 m M 2-deoxy- d -glucose) had significantly fewer adhesions of root hair tips. In addition, flushing the slide cultures with 20 m M 2-deoxy- d -glucose resulted in an immediate 4-fold reduction in frequency of tip adhesions. These results are consistent with the lectin cross-bridging model, which predicts that cell-cell adhesions would occur when trifoliin A on root hair tips contacts complementary glycosylated receptors on neighboring root hairs. 相似文献
44.
Georges Olivier 《Journal of human evolution》1980,9(8):645-649
This study is divided in two parts. The first shows that the secular trend of increasing height is far from decreasing and, on the contrary, is accelerating. The second part attacks the problem of the causes of this phenomena. It shows that the increase of stature is linked to all indicators of the conditions of life, without any one factor being predominant. On the other hand, students of the more privileged back-grounds keep on showing this secular trend, though their life standards seems to be at the optimum. Therefore, the cause and the end of this phenomena cannot be demonstrated. 相似文献
45.
46.
The cellular growth ofChlamydomonas reinhardii is modified by the addition of a total exogenous histone fraction. These modifications may be related to chloroplast DNA replication; they are different according to the different classes of histones. The H1 subfraction seems to be responsible for the effect of the total histone fraction. 相似文献
47.
Georges Rapoport André Klier Alain Billault Françoise Fargette Raymond Dedonder 《Molecular & general genetics : MGG》1979,176(2):239-245
Summary A collection of about 2500 clones containing hybrid plasmids representative of nearly the entire genome of B. subtilis 168 was established in E. coli SK1592 by using the poly(dA)·poly(dT) joining method with randomly sheared DNA fragments and plasmid pHV33, a bifunctional vector which can replicate in both E. coli and B. subtilis. Detection of cloned recombinant DNA molecules was based on the insertional inactivation of the Tc gene occurring at the unique BamHI cleavage site present in the vector plasmid.Thirty individual clones of the collection were shown to hybridize specifically with a B. subtilis rRNA probe. CCC-recombinant plasmids extracted from E. coli were pooled in lots of 100 and used to transform auxotrophic mutants of B. subtilis 168. Complementation of these auxotrophic mutations was observed for several markers such as thr, leuA, hisA, glyB and purB. In several cases, markers carried by the recombinant plasmids were lost from the plasmid and integrated into the chromosomal DNA. Loss of genetic markers from the hybrid plasmids did not occur when a rec
- recipient strain of B. subtilis was used.Abbreviations ApR
resistance to ampicillin
- TcR
resistance to tetracycline
- CmR
resistance to chloramphenicol
- CCC
covalently closed circular duplex
- Mdal
magadalton 相似文献
48.
The genomic organization of two parasitic wasps was analyzed by DNA reassociation. Cot curves revealed a pattern with three types of components. A highly repetitive DNA, accounting for 15 to 25% of the genome, was identified as satellite DNA. The moderately repetitive DNA corresponds to 26 to 42% of the genome in both species, and shows large variations in complexity, repetitive frequency and a number of sub-components between males and females. These variations are seen as resulting from DNA amplification during somatic and sexual differentiation. Dot blot analyses show that such DNA amplifications concern several types of structural and regulatory genes. The presence of repeated mobile elements was studied by the Roninson method to compare the repeated sequence patterns of Diadromus pulchellus and Eupelmus vuilleti with those of Drosophila melanogaster. The occurrence and organization of mobile elements in these Hymenoptera differ from those of the neighboring order of Diptera. The repetitive and unique components define very large genomes (1 to 3 × 109 base pairs). The genomic organization in Parasitica appears to be an extreme drosophilan type. We propose that the germinal genome of these parasitic wasps is primarily composed of satellite DNA blocks and very long stretches of unique sequences, separated by a few repeated and/or variously deleted, interspersed elements of each mobile element family. 相似文献
49.
Summary The properties of surfactin, a biosurfactant lipopeptide, were highly modified in the presence of inorganic cations. The micellization of surfactin was favoured by monovalent and, especially by divalent cations with a modification of the molecular area at the air-water interface. Haemolysis of erythrocytes by surfactin was enhanced by low concentrations of divalent cations with an increase of the binding of the lipopeptide to membrane. Inorganic ions induced conformational rearrangements probably due to ion-surfactin associations which modify the surface-active properties. 相似文献
50.
Pascal Bonnarme Michel Delattre Georges Corrieu Marcel Asther 《Applied microbiology and biotechnology》1992,37(5):670-673
Summary Lignin (LiP) and manganese peroxidase (MnP) excretion by Phanerochaete chrysosporium INA-12 was significantly increased in response to fungal extract supplementation. LiP and MnP production was increased 1.7- and 1.8-fold, respectively, with fungal extracts from agitated pellet cultures of strain INA-12, namely fungal extracts P6 and P4. In cultures supplemented with a fungal extract harvested from static cultures of strain INA-12 (fungal extract S4), LiP and MnP production was increased 1.8- and 1.6-fold, respectively. Succinate dehydrogenase activity, a mitochondrial marker, was significantly enhanced (2.7-fold) in cultures with the addition of fungal extracts.
Correspondence to: M. Asther 相似文献