全文获取类型
收费全文 | 929篇 |
免费 | 80篇 |
国内免费 | 3篇 |
出版年
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 21篇 |
2020年 | 9篇 |
2019年 | 12篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 29篇 |
2015年 | 33篇 |
2014年 | 42篇 |
2013年 | 62篇 |
2012年 | 85篇 |
2011年 | 59篇 |
2010年 | 36篇 |
2009年 | 43篇 |
2008年 | 57篇 |
2007年 | 88篇 |
2006年 | 60篇 |
2005年 | 54篇 |
2004年 | 53篇 |
2003年 | 46篇 |
2002年 | 51篇 |
2001年 | 20篇 |
2000年 | 9篇 |
1999年 | 11篇 |
1998年 | 15篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1990年 | 3篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1962年 | 1篇 |
1945年 | 3篇 |
1944年 | 3篇 |
排序方式: 共有1012条查询结果,搜索用时 15 毫秒
81.
Ryuichi Ono Yoko Kuroki Mie Naruse Masayuki Ishii Sawa Iwasaki Atsushi Toyoda Asao Fujiyama Geoff Shaw Marilyn B. Renfree Tomoko Kaneko-Ishino Fumitoshi Ishino 《DNA research》2011,18(4):211-219
In humans and mice, there are 11 genes derived from sushi-ichi related retrotransposons, some of which are known to play essential roles in placental development. Interestingly, this family of retrotransposons was thought to exist only in eutherian mammals, indicating their significant contributions to the eutherian evolution, but at least one, PEG10, is conserved between marsupials and eutherians. Here we report a novel sushi-ichi retrotransposon-derived gene, SIRH12, in the tammar wallaby, an Australian marsupial species of the kangaroo family. SIRH12 encodes a protein highly homologous to the sushi-ichi retrotransposon Gag protein in the tammar wallaby, while SIRH12 in the South American short-tailed grey opossum is a pseudogene degenerated by accumulation of multiple nonsense mutations. This suggests that SIRH12 retrotransposition occurred only in the marsupial lineage but acquired and retained some as yet unidentified novel function, at least in the lineage of the tammar wallaby. 相似文献
82.
Ricardo?S.?CeiaEmail author Hugo?L.?Sampaio Sandra?H.?Parejo Ruben?H.?Heleno Maria?L.?Arosa Jaime?A.?Ramos Geoff?M.?Hilton 《Biological invasions》2011,13(1):93-104
The invasive Clethra arborea has a dual-role in the diet of the Azores bullfinch, a critically endangered bird species endemic to the island of São Miguel (Azores, Portugal). This is a crucial winter food resource but it lowers the availability of native laurel forest species that compose most of the bird’s diet throughout the year. The removal of this and other invasive alien species is part of current laurel forest habitat restoration programmes, disregarding the impact on the Azores bullfinch population. In order to evaluate the first responses of the Azores bullfinch to habitat restoration, we studied bird diet, foraging behaviour, food availability and habitat occupancy in managed (without C. arborea) and control areas. Significant increases in the availability of native food resources in managed areas were noticeable in the diet, particularly the intake of Ilex perado ssp. azorica and Prunus lusitanica ssp. azorica flower buds. In most of the studied months birds heavily used and foraged in managed over control areas. The one exception was in December, when a resource-gap occurred in managed areas, which may be overcome in the short-term due to re-establishment of native plants following removal of invasive aliens. 相似文献
83.
Marja Simpson Geoff M. Gurr Aaron T. Simmons Steve D. Wratten David G. James Gary Leeson Helen I. Nicol 《Agricultural and Forest Entomology》2011,13(1):45-57
- 1 Plants produce natural enemy‐attracting semiochemicals known as herbivore‐induced plant volatiles (HIPV) in response to herbivore damage. Deployment of synthetic HIPV in crops could enhance the biological control of pests. To test this, six HIPV [methyl salicylate (MeSA), methyl anthranilate (MeA), methyl jasmonate (MeJA), benzaldehyde (Be), cis‐3‐hexenyl acetate (HA), cis‐hexen‐1‐ol (He)] in three concentrations (0.5%, 1.0% and 2.0% v/v) mixed with a vegetable oil adjuvant, Synertrol® (Organic Crop Protectants Pty Ltd, Australia), were sprayed onto winegrape, broccoli and sweet corn plants.
- 2 The relative abundance of insects within treated plots was assessed with non‐attracting, transparent sticky traps at varying time intervals up to 22 days after spraying.
- 3 In the vineyard experiment, Trichogrammatidae responded to Be and MeA (0.5%) and Be (1.0%); Encyrtidae and Bethylidae responded to MeA (1.0%); Scelionidae responded to all compounds at 1.0% and 2.0%; and predatory insects responded to MeA. In sweet corn, parasitoids as a group and Encyrtidae responded to MeA (0.5%); Braconidae responded to all compounds at 0.5% and Synertrol‐only; thrips responded to all compounds at 0.5% and 1.0%; while all parasitoids responded to all compounds at 0.5% and 1.0% and Synertrol‐only. In broccoli, parasitoids as a group and Scelionidae responded to Be, HA, He and Synertrol‐only; Trichogrammatidae responded to Be (0.5%), He (0.5% and 1.0%), MeJA (1.0%) and MeSA (0.5%); and thrips responded to all compounds at to 0.5% and 1.0%.
- 4 Significant attraction of insects occurred up to 6 days after the HIPV application, suggesting that plants may have been induced to produce endogenous volatiles that attracted insects over an extended period.
- 5 The results obtained are discussed in relation to the potential utility of synthetic HIPV to enhance the biological control of pests.
84.
The mechanisms behind the evolution and maintenance of conspicuous visible polymorphisms comprising tens of morphs present a challenge to evolutionary theory. However, for cryptic forms Endler (Evol. Biol., 11, 1978, 319) conjectured that complex backgrounds facilitate polymorphism because in such habitats there are several ways to resemble the resting surface. We use computer simulation to explore the evolution of cryptic morphs on increasingly complex backgrounds under regimes that include selection for crypsis, apostatic predation and dietary wariness. We show that there is a monotonic increase in the number of morphs evolving in a population as the potential number of cryptic morphs increases. The relationship is very weak with selection for crypsis alone, but much stronger with the addition of apostatic selection. In contrast, when dietary wariness is added to the model the plot of number of morphs maintained, as a function of the potential number of cryptic forms available, is minimized at an intermediate number of cryptic forms, i.e. is V-shaped. These counter-intuitive patterns are robust to varying strengths of apostatic selection and different implementations of dietary wariness, and are more pronounced when predator and prey generation lengths are similar. 相似文献
85.
Nerve Growth Factor is an essential protein that supports neuronal survival during development and influences neuronal function throughout adulthood, both in the central and peripheral nervous system. The unprocessed precursor of NGF, proNGF, seems to be endowed with biological functions distinct from those of the mature protein, such as chaperone-like activities and apoptotic and/or neurotrophic properties. We have previously suggested, based on Small Angle X-ray Scattering data, that recombinant murine proNGF has features typical of an intrinsically unfolded protein. Using complementary biophysical techniques, we show here new evidence that clarifies and widens this hypothesis through a detailed comparison of the structural properties of NGF and proNGF. Our data provide direct information about the dynamic properties of the pro-peptide and indicate that proNGF assumes in solution a compact globular conformation. The N-terminal pro-peptide extension influences the chemical environment of the mature protein and protects the protein from proteolytic digestion. Accordingly, we observe that unfolding of proNGF involves a two-steps mechanism. The distinct structural properties of proNGF as compared to NGF agree with and rationalise a different functional role of the precursor. 相似文献
86.
We investigate an instance of conflict between mates over the sex ratio of their brood. We construct a kin-selection model for the evolution of the sex ratio assuming local resource competition (LRC) among females. We explore two basic scenarios: (a) the case where parents make simultaneous sex-ratio decisions (the simultaneous allocation model); and (b) the case where parental sex-ratio decisions occur one after the other (the sequential allocation model). In the simultaneous investment model, resolution of the conflict between mates depends on the extent to which relative paternal contribution influences the brood sex ratio. In the sequential allocation model, fathers determine primary sex-ratio through fertilization bias; then mothers modify the paternal sex-ratio decision by adjusting the level of investment of some resource that contributes to offspring survival. Under the sequential model, a compromise is always achieved; however this compromise favours one perspective or the other, depending on the extent to which maternal investment influences offspring survival. 相似文献
87.
88.
Macpherson JL Boyd MP Arndt AJ Todd AV Fanning GC Ely JA Elliott F Knop A Raponi M Murray J Gerlach W Sun LQ Penny R Symonds GP Carr A Cooper DA 《The journal of gene medicine》2005,7(5):552-564
BACKGROUND: An anti-HIV-1 tat ribozyme, termed Rz2, has been shown to inhibit HIV-1 infection/replication and to decrease HIV-1-induced pathogenicity in T-lymphocyte cell lines and normal peripheral blood T-lymphocytes. We report here the results of a phase I gene transfer clinical trial using Rz2. METHODS: Apheresis was used to obtain a peripheral blood cell population from each of four HIV-negative donors. After enrichment for CD4+ T-lymphocytes, ex vivo expansion and genetic manipulation (approximately equal aliquots of the cells were transduced with the ribozyme-containing (RRz2) and the control (LNL6) retroviral vector), these cells were infused into the corresponding HIV-1-positive twin recipient. Marking was assessed over an initial 24-week period and in total over an approximate 4-year period. RESULTS: The gene transfer procedure was shown to be safe, and technically feasible. Both RRz2- and LNL6-gene-containing peripheral blood mononuclear cells (PBMC) were detected at all time points examined to 4 years. There was concomitant gene construct expression in the absence of the need for ex vivo peripheral blood cell stimulation and there was no evidence of immune elimination of the neoR T-lymphocytes nor of silencing of the Moloney murine leukemia virus long terminal repeat. CONCLUSIONS: The proof of principle results reported here demonstrate safety and feasibility of this type of gene transfer approach. While not specifically tested, T-lymphocytes containing an anti-HIV gene construct may impact on HIV-1 viral load and CD4+ T-lymphocyte count, potentially representing a new therapeutic modality for HIV-1 infection. 相似文献
89.
R. Geoff B.?SmithEmail author J. Doland?Nichols Jerome K.?Vanclay 《Biodiversity and Conservation》2005,14(10):2447-2463
In subtropical rainforest in eastern Australia, changes in the diversity of trees were compared under natural conditions and
eight silvicultural regimes over 35 years. In the treated plots basal area remaining after logging ranged from 12 to 58 m^{2}/ha.
In three control plots richness differed little over this period. In the eight treated plots richness per plot generally declined
after intervention and then gradually increased to greater than original diversity. After logging there was a reduction in
richness per plot and an increase in species richness per stem in all but the lightest selective treatments. The change in
species diversity was related to the intensity of the logging, however the time taken for species richness to return to pre-logging
levels was similar in all silvicultural treatments and was not effected by the intensity of treatment. These results suggest
that light selective logging in these forests mainly affects dominant species. The return to high diversity after only a short
time under all silvicultural regimes suggests that sustainability and the manipulation of species composition for desired
management outcomes is possible. 相似文献
90.
Anduleit K Sutton G Diprose JM Mertens PP Grimes JM Stuart DI 《Protein science : a publication of the Protein Society》2005,14(10):2741-2743
Many insect viruses survive for long periods by occlusion within robust crystalline polyhedra composed primarily of a single polyhedrin protein. We show that two different virus families form polyhedra which, despite lack of sequence similarity in the virally encoded polyhedrin protein, have identical cell constants and a body-centered cubic lattice. It is almost inconceivable that this could have arisen by chance, suggesting that the crystal lattice has been preserved because it is particularly well-suited to its function of packaging and protecting viruses. 相似文献