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41.
Irfan Farabi Hayat Manuel Plan Birgitta E. Ebert Geoff Dumsday Claudia E. Vickers Bingyin Peng 《Microbial biotechnology》2021,14(6):2627-2642
The yeast Saccharomyces cerevisiae uses the pyruvate dehydrogenase-bypass for acetyl-CoA biosynthesis. This relatively inefficient pathway limits production potential for acetyl-CoA-derived biochemical due to carbon loss and the cost of two high-energy phosphate bonds per molecule of acetyl-CoA. Here, we attempted to improve acetyl-CoA production efficiency by introducing heterologous acetylating aldehyde dehydrogenase and phosphoketolase pathways for acetyl-CoA synthesis to enhance production of the sesquiterpene trans-nerolidol. In addition, we introduced auxin-mediated degradation of the glucose-dependent repressor Mig1p to allow induced expression of GAL promoters on glucose so that production potential on glucose could be examined. The novel genes that we used to reconstruct the heterologous acetyl-CoA pathways did not sufficiently complement the loss of endogenous acetyl-CoA pathways, indicating that superior heterologous enzymes are necessary to establish fully functional synthetic acetyl-CoA pathways and properly explore their potential for nerolidol synthesis. Notwithstanding this, nerolidol production was improved twofold to a titre of ˜ 900 mg l−1 in flask cultivation using a combination of heterologous acetyl-CoA pathways and Mig1p degradation. Conditional Mig1p depletion is presented as a valuable strategy to improve the productivities in the strains engineered with GAL promoters-controlled pathways when growing on glucose. 相似文献
42.
W. Ellis Penning Marit Mjelde Bernard Dudley Seppo Hellsten Jenica Hanganu Agnieszka Kolada Marcel van den Berg Sandra Poikane Geoff Phillips Nigel Willby Frauke Ecke 《Aquatic Ecology》2008,42(2):237-251
Aquatic macrophytes are one of the biological quality elements in the Water Framework Directive (WFD) for which status assessments
must be defined. We tested two methods to classify macrophyte species and their response to eutrophication pressure: one based
on percentiles of occurrence along a phosphorous gradient and another based on trophic ranking of species using Canonical
Correspondence Analyses in the ranking procedure. The methods were tested at Europe-wide, regional and national scale as well
as by alkalinity category, using 1,147 lakes from 12 European states. The grouping of species as sensitive, tolerant or indifferent
to eutrophication was evaluated for some taxa, such as the sensitive Chara spp. and the large isoetids, by analysing the (non-linear) response curve along a phosphorous gradient. These thresholds
revealed in these response curves can be used to set boundaries among different ecological status classes. In total 48 taxa
out of 114 taxa were classified identically regardless of dataset or classification method. These taxa can be considered the
most consistent and reliable indicators of sensitivity or tolerance to eutrophication at European scale. Although the general
response of well known indicator species seems to hold, there are many species that were evaluated differently according to
the database selection and classification methods. This hampers a Europe-wide comparison of classified species lists as used
for the status assessment within the WFD implementation process. 相似文献
43.
A new species of Taeniastrotos is described from an ariid host, Cathrops spixii, caught in southern Brazil. It is the first member of the genus to be recorded from the Atlantic Ocean and can be distinguished from its four known congeners by the setation of the terminal endopodal segment of the fourth leg. 相似文献
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45.
Andrew Pullin Geoff Frampton Rob Jongman Christian Kohl Barbara Livoreil Alexandra Lux György Pataki Gillian Petrokofsky Aranka Podhora Heli Saarikoski Luis Santamaria Stefan Schindler Isabel Sousa-Pinto Marie Vandewalle Heidi Wittmer 《Biodiversity and Conservation》2016,25(7):1285-1300
Responding to different questions generated by biodiversity and ecosystem services policy or management requires different forms of knowledge (e.g. scientific, experiential) and knowledge synthesis. Additionally, synthesis methods need to be appropriate to policy context (e.g. question types, budget, timeframe, output type, required scientific rigour). In this paper we present a range of different methods that could potentially be used to conduct a knowledge synthesis in response to questions arising from knowledge needs of decision makers on biodiversity and ecosystem services policy and management. Through a series of workshops attended by natural and social scientists and decision makers we compiled a range of question types, different policy contexts and potential methodological approaches to knowledge synthesis. Methods are derived from both natural and social sciences fields and reflect the range of question and study types that may be relevant for syntheses. Knowledge can be available either in qualitative or quantitative form and in some cases also mixed. All methods have their strengths and weaknesses and we discuss a sample of these to illustrate the need for diversity and importance of appropriate selection. To summarize this collection, we present a table that identifies potential methods matched to different combinations of question types and policy contexts, aimed at assisting teams undertaking knowledge syntheses to select appropriate methods. 相似文献
46.
Marja Simpson Geoff M. Gurr Aaron T. Simmons Steve D. Wratten David G. James Gary Leeson Helen I. Nicol 《Agricultural and Forest Entomology》2011,13(1):45-57
- 1 Plants produce natural enemy‐attracting semiochemicals known as herbivore‐induced plant volatiles (HIPV) in response to herbivore damage. Deployment of synthetic HIPV in crops could enhance the biological control of pests. To test this, six HIPV [methyl salicylate (MeSA), methyl anthranilate (MeA), methyl jasmonate (MeJA), benzaldehyde (Be), cis‐3‐hexenyl acetate (HA), cis‐hexen‐1‐ol (He)] in three concentrations (0.5%, 1.0% and 2.0% v/v) mixed with a vegetable oil adjuvant, Synertrol® (Organic Crop Protectants Pty Ltd, Australia), were sprayed onto winegrape, broccoli and sweet corn plants.
- 2 The relative abundance of insects within treated plots was assessed with non‐attracting, transparent sticky traps at varying time intervals up to 22 days after spraying.
- 3 In the vineyard experiment, Trichogrammatidae responded to Be and MeA (0.5%) and Be (1.0%); Encyrtidae and Bethylidae responded to MeA (1.0%); Scelionidae responded to all compounds at 1.0% and 2.0%; and predatory insects responded to MeA. In sweet corn, parasitoids as a group and Encyrtidae responded to MeA (0.5%); Braconidae responded to all compounds at 0.5% and Synertrol‐only; thrips responded to all compounds at 0.5% and 1.0%; while all parasitoids responded to all compounds at 0.5% and 1.0% and Synertrol‐only. In broccoli, parasitoids as a group and Scelionidae responded to Be, HA, He and Synertrol‐only; Trichogrammatidae responded to Be (0.5%), He (0.5% and 1.0%), MeJA (1.0%) and MeSA (0.5%); and thrips responded to all compounds at to 0.5% and 1.0%.
- 4 Significant attraction of insects occurred up to 6 days after the HIPV application, suggesting that plants may have been induced to produce endogenous volatiles that attracted insects over an extended period.
- 5 The results obtained are discussed in relation to the potential utility of synthetic HIPV to enhance the biological control of pests.
47.
Ricardo?S.?CeiaEmail author Hugo?L.?Sampaio Sandra?H.?Parejo Ruben?H.?Heleno Maria?L.?Arosa Jaime?A.?Ramos Geoff?M.?Hilton 《Biological invasions》2011,13(1):93-104
The invasive Clethra arborea has a dual-role in the diet of the Azores bullfinch, a critically endangered bird species endemic to the island of São Miguel (Azores, Portugal). This is a crucial winter food resource but it lowers the availability of native laurel forest species that compose most of the bird’s diet throughout the year. The removal of this and other invasive alien species is part of current laurel forest habitat restoration programmes, disregarding the impact on the Azores bullfinch population. In order to evaluate the first responses of the Azores bullfinch to habitat restoration, we studied bird diet, foraging behaviour, food availability and habitat occupancy in managed (without C. arborea) and control areas. Significant increases in the availability of native food resources in managed areas were noticeable in the diet, particularly the intake of Ilex perado ssp. azorica and Prunus lusitanica ssp. azorica flower buds. In most of the studied months birds heavily used and foraged in managed over control areas. The one exception was in December, when a resource-gap occurred in managed areas, which may be overcome in the short-term due to re-establishment of native plants following removal of invasive aliens. 相似文献
48.
Australian native flowering plants enhance the longevity of three parasitoids of brassica pests 下载免费PDF全文
Sunita Pandey Azizur Rahman Geoff M. Gurr 《Entomologia Experimentalis et Applicata》2018,166(4):265-276
Floral resources from native plants that are adapted to the local environment could be more advantageous than the use of nonnative plants. In Australia, there is a dearth of information on the benefits of native plants to natural enemies and their selectivity against pests. Accordingly, we examined the longevity of the parasitoids Diaeretiella rapae (McIntosh) and Cotesia glomerata (L.) (both Hymenoptera: Braconidae), and Diadegma semiclausum (Hellen) (Hymenoptera: Ichneumonidae) exposed to flowering shoots from Australian native plants which was compared with the nonnative buckwheat (Fagopyrum esculentum), often used in conservation biological control. Longevity of parasitoids was significantly enhanced by the Australian natives Westringia fruticosa, Mentha satureioides, Callistemon citrinus, Leptospermum cv. ‘Rudolph’, Grevillea cv. ‘Bronze Rambler’, Myoporum parvifolium, Lotus australis, and nonnative F. esculentum. The highest mean survival by native plant species was 3.4× higher for D. rapae with Leptospermum sp. and 4.3× higher for D. semiclausum with M. parvifolium. For C. glomerata, Grevillea sp. increased longevity by 6.9× compared with water only. Longevity of Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major crop pest, was enhanced by all plants against which it was screened except Acacia baileyana, a species that had no effect on parasitoid longevity. Several Australian native plant species that benefit parasitoids were identified. None of the plant species provided a selective benefit to the parasitoid D. semiclausum compared with its host P. xylostella; however, the benefit of M. parvifolium and Grevillea sp. on the longevity of D. semiclausum was relatively higher compared with the pest. These results suggest the need for field studies to determine whether native Australian plants increase P. xylostella impact in nearby brassica crops. 相似文献
49.
Impacts of the invasive fungus Austropuccinia psidii (myrtle rust) on three Australian Myrtaceae species of coastal swamp woodland 下载免费PDF全文
Laura Fernandez Winzer Angus J. Carnegie Geoff S. Pegg Michelle R. Leishman 《Austral ecology》2018,43(1):56-68
Exotic fungal pathogens can substantially affect individuals and populations of susceptible native plant species, potentially resulting in changes in community structure and composition. Austropuccinia psidii (myrtle rust) is a pathogenic fungus native to South America that affects species in the plant family Myrtaceae. The pathogen was introduced accidentally to Australia and first detected in NSW in April 2010. Ecological impacts have been poorly studied in the native range of A. psidii and even less in its Australian introduced range. In order to assess the potential impact of A. psidii on coastal swamp woodland, two glasshouse experiments were conducted using three co‐occurring species: Melaleuca quinquenervia, Leptospermum laevigatum and Baeckea linifolia. Plants of each species were grown individually (Experiment 1) and in mixed species assemblages (Experiment 2), with half inoculated with A. psidii and the other half remaining as controls. Infection level was assessed and impact on seedling survival and growth recorded. In both experiments L. laevigatum and M. quinquenervia seedlings were heavily infected and showed high degrees of susceptibility with negative effects on growth (height, biomass and number of leaves). In contrast, no B. linifolia seedling presented visible symptoms of disease, although seedlings showed reduced growth. Melaleuca quinquenervia seedlings had greater infection levels and suffered greater growth reductions than L. laevigatum in both experiments. However, there was no significant difference in the relative abundance of the three species in the mixed‐species experiment. This study provides a better understanding of the potential impacts of A. psidii in this vegetation community and has significant implications for the conservation and management of Australian Myrtaceae‐dominated plant communities generally. 相似文献
50.