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11.
Performance of a constructed wetland in treating brackish wastewater from commercial recirculating and super-intensive shrimp growout systems 总被引:6,自引:0,他引:6
A recirculating aquaculture system was developed for treating Pacific white shrimp (Litopenaeus vannamei) production wastewater using an integrated vertical-flow (IVF) and five connected integrated horizontal flow (IHF) constructed wetlands as water treatment filters for mesohaline conditions (8.25‰-8.26‰ salinity). The constructed wetlands demonstrated the ability to reduce total nitrogen, total ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total phosphorous, chemical oxygen demand, and total suspended solids to levels significantly lower than those in effluents from culture tanks. Various water quality parameters in the culture tanks were deemed suitable for shrimp culture. The actual ratio of wetland area (A(w)) to culture tank area (A(t)) was 1.1439, and the estimated optimal ratio A(w)/A(t) was approximately 1. The IVF-IHF wetlands showed flexibility and reliability in consistently removing the main pollutants from commercial recirculating and super-intensive shrimp growout systems throughout the culture period. 相似文献
12.
Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken 总被引:4,自引:0,他引:4
WANG Genyu YAN Bingxue DENG Xuemei LI Changlü HU Xiaoxiang & Li Ning .State Key Laboratory for Agrobiotechnology China Agricultural University Beijing China .Department of Animal Genetics Breeding College of Animal Science Technology China Agricultural University Beijing China 《中国科学:生命科学英文版》2005,48(2):187-194
13.
Pan Liao Wei Zhou Lin Zhang Jing Wang Xiangming Yan Yan Zhang Ran Zhang Li Li Genyu Zhou Guoyin Kai 《Acta Physiologiae Plantarum》2009,31(3):565-572
The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate (MVA), which is the
first committed step in MVA pathway for isoprenoid biosynthesis in plants. In this study, a full-length cDNA encoding HMGR
was isolated from Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE) for the first time, which was designated as SmHMGR (GenBank Accession No.EU680958). The full-length cDNA of SmHMGR was 2,115 bp containing a 1,695 bp open reading frame (ORF) encoding a polypeptide of 565 amino acids. Bioinformatic analyzes
revealed that the deduced SmHMGR had extensive homology with other plant HMGRs contained two transmembrane domains and a catalytic domain. Molecular modeling
showed that SmHMGR is a new HMGR with a spatial structure similar to other plant HMGRs. Phylogenetic tree analysis indicated
that SmHMGR belongs to the plant HMGR super-family and has the closest relationship with HMGR from Picrorhiza kurrooa. Expression pattern analysis implied that SmHMGR expressed highest in root, followed by stem and leaf. The expression of SmHMGR could be up-regulated by salicylic acid (SA) and methyl jasmonate (MeJA), suggesting that SmHMGR was elicitor-responsive. This work will be helpful to understand more about the role of HMGR involved in the tanshinones
biosynthesis at the molecular level. 相似文献
14.
Genyu Wang Pengfei Wu Ya Liu Shuo Mi Shuai Mai Chunkai Gu Gehua Wang Hongjuan Liu Jianan Zhang Børre Tore Børresen Evy Mellemsæther Hans Kristian Kotlar 《Applied microbiology and biotechnology》2015,99(20):8803-8813
Butanol-producing microorganisms are all obligate anaerobes. In this study, a unique symbiotic system TSH06 was isolated to be capable of producing butanol under non-anaerobic condition. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal RNA (rRNA) revealed that two strains coexist in TSH06. The two strains were identical to Clostridium acetobutylicum and Bacillus cereus, respectively. They were isolated individually and named as C. acetobutylicum TSH1 and B. cereus TSH2. C. acetobutylicum TSH1 is a butanol-producing, obligate anaerobic strain. Facultative anaerobic B. cereus TSH2 did not possess the ability of butanol production; however, it offered C. acetobutylicum TSH1 the viability under non-anaerobic condition. Moreover, B. cereus TSH2 enhanced butanol yield and speed of fermentation. TSH06 produced 12.97 g/L butanol and 15.39 g/L total solvent under non-anaerobic condition, which is 25 and 24 %, respectively, higher than those of C. acetobutylicum TSH1. In addition, TSH06 produced butanol faster under non-anaerobic condition than under anaerobic condition. Butanol accounted for more than 80 % of total solvent, which is higher than the known report. TSH06 was stable during passage. In all, TSH06 is a promising candidate for industrialisation of biobutanol with high yield, high butanol proportion, easy-handling and time-saving system. These results demonstrated the potential advantage of symbiosis. This study also provides a promising strategy for butanol fermentation. 相似文献