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591.
Activation of Glycosidases as a Consequence of Infection Stress in Fusarium Wilt of Tomato 总被引:1,自引:0,他引:1
Changes of β-1,3-glucanase, chitinase, β-1,4-glucosidase and N-acetylglucosaminidase activity have been investigated in relation to the development of symptoms and colonization by the pathogen in roots, stems and leaves of susceptible (‘Improved, Pearson’) and resistant (‘Improved Pearson VF11’) tomato plants infected by Fusarium oxysporum f. sp. lycopersici. Glycosidase activities increased after inoculation to different extents depending on the plant part and cultivar. Increases were always higher in susceptible than in resistant plants. Changes in the β-1,3-glucanase activity after inoculation were particularly large in stems of infected plants. In contrast, chitinase activity increased more in roots than in stems. The β-1,3-glucosidase and chitinase activity decreased slightly from the basal to the apical third of stems. The trend of changes of the glycosidase activity generally were well related with the severity of disease symptoms and the fungal colonization of basal stem segments. There was no evidence that the increase of glycosidase activity after the infection was directly related with the resistance to Fusarium wilt in tomato. 相似文献
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593.
Site occupancy‐detection models (SODMs) are statistical models widely used for biodiversity surveys where imperfect detection of species occurs. For instance, SODMs are increasingly used to analyse environmental DNA (eDNA) data, taking into account the occurrence of both false‐positive and false‐negative errors. However, species occurrence data are often characterized by spatial and temporal autocorrelation, which might challenge the use of standard SODMs. Here we reviewed the literature of eDNA biodiversity surveys and found that most of studies do not take into account spatial or temporal autocorrelation. We then demonstrated how the analysis of data with spatial or temporal autocorrelation can be improved by using a conditionally autoregressive SODM, and show its application to environmental DNA data. We tested the autoregressive model on both simulated and real data sets, including chronosequences with different degrees of autocorrelation, and a spatial data set on a virtual landscape. Analyses of simulated data showed that autoregressive SODMs perform better than traditional SODMs in the estimation of key parameters such as true‐/false‐positive rates and show a better discrimination capacity (e.g., higher true skill statistics). The usefulness of autoregressive SODMs was particularly high in data sets with strong autocorrelation. When applied to real eDNA data sets (eDNA from lake sediment cores and freshwater), autoregressive SODM provided more precise estimation of true‐/false‐positive rates, resulting in more reasonable inference of occupancy states. Our results suggest that analyses of occurrence data, such as many applications of eDNA, can be largely improved by applying conditionally autoregressive specifications to SODMs. 相似文献
594.
Benoit Diringer Krizia Pretell Ricardo Avellan Cesar Chanta Virna Cedeo Gabriele Gentile 《Ecology and evolution》2019,9(8):4392-4402
Wild populations of the pustulose ark, Anadara tuberculosa (Bivalvia), an emblematic species of the East Pacific mangrove ecosystem declined in South American countries (Colombia, Ecuador, and Peru) mainly due to overharvesting and habitat loss or degradation. Understanding the genetic aspects of geographic variations and population structure of A. tuberculosa, currently unknown, appears as a priority to fishery authorities in order to elaborate integrated and collaborative conservation policies for fishery management, aquaculture, and stock enhancement programs. We used mtDNA sequence data to investigate haplotype diversity, genetic structure, and demography of A. tuberculosa. Results indicate genetic homogeneity of populations distributed north and south of the equator, respectively. However, statistically significant differentiation emerged between northern and southern populations with pairwise фST values ranging between 0.036 and 0.092. The oceanic current system acting in the area (Panama Current and Humboldt Current) might play a role in limiting the larval dispersal of the species, still poorly understood. Demography reconstruction supported recent population expansion, possibly started after last glacial maximum. Our results would suggest separate and independent management of populations north and south of the equator. 相似文献
595.
A mutant of Saccharomyces cerevisiae, DW137, isolated after treatment of a wild-type strain with ICR-170. The mutant was respiration-deficient and showed abnormal cell division when grown at 30 degrees C. In addition, the mutant was temperature-sensitive and underwent lysis when grown at 37 degrees C. Random spore analysis, induced reversion profiles, and complementation analysis indicated that the abnormal phenotypes were under the control of a single recessive mutation caused by a base-pair substitution in a nuclear gene. Macromolecular analysis of the mutant at permissive and restrictive temperatures showed that at restrictive temperatures the mutant cannot synthesize DNA. Surprisingly, at restrictive temperatures, protein synthesis in the mutant continued at a rate greater than that observed at permissive temperatures. Cell death and lysis of the mutant could be prevented by treatment of cultures with cycloheximide, an inhibitor of protein synthesis. The data suggest that the abnormally high rate of protein synthesis and the inability to synthesize DNA are jointly responsible for death of the cells, and most probably play and integrating role in the incipient cell lysis. 相似文献
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598.
This study examines the application of population statistics to laboratory-derived toxicological data for the purpose of developing a predictive model that assesses the population consequences of pollutant and environmentally-induced stress. Life tables are used to calculate age-specific survivorship, fecundity, intrinsic rate of population increase (r), and reproductive value (Va), for populations ofMysidopsis bahia chronically exposed, in separate tests, to mercury and nickel. The population statistics, r, and Va, and pollutant dose are defined quantitatively, and the critical value (r = 0) for the intrinsic rate of population increase is compared with traditional toxicological measures of acute and chronic toxicity.The effects of an environmental variable, predation, significantly reduce the critical value (r = 0) for the intrinsic rate of growth and demonstrate the interactions of multiple stressors. The use of the population statistic, r, is recommended for estimating the ecological significance of toxicological data and for facilitating the assessment of interactions between pollutant-stressed populations and dynamic environmental pressures.Contribution No. 258 of the U.S. Environmental Protection Agency. 相似文献
599.
Matteo Elio Siesa Raoul Manenti Emilio Padoa-Schioppa Fiorenza De Bernardi Gentile Francesco Ficetola 《Biological invasions》2011,13(9):2147-2160
Complex spatial dynamics are frequent in invasive species; analyzing distribution patterns can help to understand the mechanisms
driving invasions. We used different spatial regression techniques to evaluate processes determining the invasion of the red
swamp crayfish Procambarus clarkii. We evaluated four a priori hypotheses on processes that may determine crayfish invasion: landscape alteration, connectivity,
wetland suitability for abiotic and biotic features. We assessed the distribution of P. clarkii in 119 waterbodies in a recently invaded area. We used spatially explicit statistical techniques (spatial eigenvector mapping,
generalized additive models, Bayesian intrinsic conditional autoregressive models) within an information-theoretic framework
to assess the support of hypotheses; we also analyzed the pattern of spatial autocorrelation of data, model residuals, and
eigenvectors. We found strong agreement between the results of spatial eigenvector mapping and Bayesian autoregressive models.
Procambarus clarkii was significantly associated with the largest, permanent wetlands. Additive models suggested also association with human-dominated
landscapes, but tended to overfit data. The results indicate that abiotic wetlands features and landscape alteration are major
drivers of the species’ distribution. Species distribution data, residuals of ordinary least squares regression, and spatial
eigenvectors all showed positive and significant spatial autocorrelation at distances up to 2,500 m; this may be caused by
the dispersal ability of the species. Our analyses help to understand the processes determining the invasion and to identify
the areas most at risk where screening and early management efforts can be focused. The comparison of multiple spatial techniques
allows a robust assessment of factors determining complex distribution patterns. 相似文献
600.
Melotto Andrea Ficetola Gentile Francesco Pennati Roberta Ancona Nicoletta Manenti Raoul 《Biological invasions》2021,23(12):3777-3793
Biological Invasions - During biotic invasions, native communities are abruptly exposed to novel and often severe selective pressures. The lack of common evolutionary history with invasive... 相似文献