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111.
Maria Cristina Thaller Luciana Migliore Cruz Marquez Washington Tapia Virna Cede?o Gian Maria Rossolini Gabriele Gentile 《PloS one》2010,5(2)
Background
Antibiotic resistance, evolving and spreading among bacterial pathogens, poses a serious threat to human health. Antibiotic use for clinical, veterinary and agricultural practices provides the major selective pressure for emergence and persistence of acquired resistance determinants. However, resistance has also been found in the absence of antibiotic exposure, such as in bacteria from wildlife, raising a question about the mechanisms of emergence and persistence of resistant strains under similar conditions, and the implications for resistance control strategies. Since previous studies yielded some contrasting results, possibly due to differences in the ecological landscapes of the studied wildlife, we further investigated this issue in wildlife from a remote setting of the Galapagos archipelago.Methodology/Principal Findings
Screening for acquired antibiotic resistance was carried out in commensal enterobacteria from Conolophus pallidus, the terrestrial iguana of Isla Santa Fe, where: i) the abiotic conditions ensure to microbes good survival possibilities in the environment; ii) the animal density and their habits favour microbial circulation between individuals; and iii) there is no history of antibiotic exposure and the impact of humans and introduced animal species is minimal except for restricted areas. Results revealed that acquired antibiotic resistance traits were exceedingly rare among bacteria, occurring only as non-dominant strains from an area of minor human impact.Conclusions/Significance
Where both the exposure to antibiotics and the anthropic pressure are minimal, acquired antibiotic resistance traits are not normally found in bacteria from wildlife, even if the ecological landscape is highly favourable to bacterial circulation among animals. Monitoring antibiotic resistance in wildlife from remote areas could also be a useful tool to evaluate the impact of anthropic pressure. 相似文献112.
Global patterns of β‐diversity along the phylogenetic time‐scale: The role of climate and plate tectonics 下载免费PDF全文
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Tainá CC Monte Rosana Gentile Juberlan Garcia Ester Mota Jeannie N Santos Arnaldo Maldonado Júnior 《Memórias do Instituto Oswaldo Cruz》2014,109(8):1057-1063
Angiostrongylus cantonensis is the etiologic agent of eosinophilic
meningoencephalitis in humans. Cases have been recorded in many parts of the world,
including Brazil. The aim of this study was to compare the differences in the biology
and morphology of two different Brazilian haplotypes of A. : ac8 and
ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents
at the beginning of the patent period was observed for ac9 haplotype and compared to
the total of L1 larvae eliminated, there was a significant difference between the two
haplotypes. The ac9 haplotype showed a significant difference in the proportion of
female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The
morphometric analysis showed that male and female specimens isolated from ac8
haplotype were significantly larger with respect to body length, oesophagus length,
spicule length (male) and distance from the anus to the rear end (female) compared to
specimens from ac9. The morphological analysis by light microscopy showed little
variation in the level of bifurcations at the lateral rays in the right lobe of the
copulatory bursa between the two haplotypes. The biological, morphological and
morphometric variations observed between the two haplotypes agree with the observed
variation at the molecular level using the cytochrome oxidase subunit I marker and
reinforce the possible influence of geographical isolation on the development of
these haplotypes. 相似文献
115.
Background and Aims
An intense pollen–pistil interaction precedes fertilization. This interaction is of particular relevance in agronomically important species where seeds or fruits are the edible part. Over time some agronomically species have been selected for the ability to produce fruit without seeds. While this phenomenon is critical for commercial production in some species, very little is known about the events behind the production of seedless fruit. In this work, the relationship between pollen–pistil interaction and the onset of fruiting was investigated in citrus mandarin.Methods
Pistils were sequentially examined in hand-pollinated flowers paying attention to pollen-tube behaviour, and to cytochemical changes along the pollen-tube pathway. To evaluate which of these changes were induced by pollination/fertilization and which were developmentally regulated, pollinated and unpollinated pistils were compared. Also the onset of fruiting was timed and changes in the ovary examined.Key Results
Conspicuous changes occurred in the pistil along the pollen-tube pathway, which took place in a basipetal way encompassing the timing of pollen-tube growth. However, these changes appear to be developmentally regulated as they happened in the same way and at the same time in unpollinated flowers. Moreover, the onset of fruiting occurred prior to fertilization and the very same changes could be observed in unpollinated flowers.Conclusions
Pollen–pistil interaction in citrus showed similarities with unrelated species and families belonging to other taxa. The uncoupling of the reproductive and fruiting processes accounts for the parthenocarpic ability of unpollinated flowers to produce fruit in citrus. However, the maintenance of a functional reproductive process reflects the potential to produce seeded fruits, providing a basis for the understanding of the production of seeded or unseeded fruits and further understanding of the process of parthenocarpy in other species. 相似文献116.
Gentile MA Melchiorre S Emolo C Moschioni M Gianfaldoni C Pancotto L Ferlenghi I Scarselli M Pansegrau W Veggi D Merola M Cantini F Ruggiero P Banci L Masignani V 《The Journal of biological chemistry》2011,286(16):14588-14597
Streptococcus pneumoniae expresses on its surface adhesive pili, involved in bacterial attachment to epithelial cells and virulence. The pneumococcal pilus is composed of three proteins, RrgA, RrgB, and RrgC, each stabilized by intramolecular isopeptide bonds and covalently polymerized by means of intermolecular isopeptide bonds to form an extended fiber. RrgB is the pilus scaffold subunit and is protective in vivo in mouse models of sepsis and pneumonia, thus representing a potential vaccine candidate. The crystal structure of a major RrgB C-terminal portion featured an organization into three independently folded protein domains (D2-D4), whereas the N-terminal D1 domain (D1) remained unsolved. We have tested the four single recombinant RrgB domains in active and passive immunization studies and show that D1 is the most effective, providing a level of protection comparable with that of the full-length protein. To elucidate the structural features of D1, we solved the solution structure of the recombinant domain by NMR spectroscopy. The spectra analysis revealed that D1 has many flexible regions, does not contain any intramolecular isopeptide bond, and shares with the other domains an Ig-like fold. In addition, we demonstrated, by site-directed mutagenesis and complementation in S. pneumoniae, that the D1 domain contains the Lys residue (Lys-183) involved in the formation of the intermolecular isopeptide bonds and pilus polymerization. Finally, we present a model of the RrgB protein architecture along with the mapping of two surface-exposed linear epitopes recognized by protective antisera. 相似文献
117.
Jack?A?TuszynskiEmail author Philip?Winter Diana?White Chih-Yuan?Tseng Kamlesh?K?Sahu Francesco?Gentile Ivana?Spasevska Sara?Ibrahim?Omar Niloofar?Nayebi Cassandra?DM?Churchill Mariusz?Klobukowski Rabab?M?Abou?El-Magd 《Theoretical biology & medical modelling》2014,11(1):52
A variety of topics are reviewed in the area of mathematical and computational modeling in biology, covering the range of scales from populations of organisms to electrons in atoms. The use of maximum entropy as an inference tool in the fields of biology and drug discovery is discussed. Mathematical and computational methods and models in the areas of epidemiology, cell physiology and cancer are surveyed. The technique of molecular dynamics is covered, with special attention to force fields for protein simulations and methods for the calculation of solvation free energies. The utility of quantum mechanical methods in biophysical and biochemical modeling is explored. The field of computational enzymology is examined. 相似文献
118.
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120.
Citrus phylogeny and genetic origin of important species as investigated by molecular markers 总被引:19,自引:0,他引:19
E. Nicolosi Z. N. Deng A. Gentile S. La Malfa G. Continella E. Tribulato 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1155-1166
Citrus phylogeny was investigated using RAPD, SCAR and cpDNA markers. The genotypes analyzed included 36 accessions belonging
to Citrus together with 1 accession from each of the related genera Poncirus, Fortunella, Microcitrus and Eremocitrus. Phylogenetic analysis with 262 RAPDs and 14 SCARs indicated that Fortunella is phylogenetically close to Citrus while the other three related genera are distant from Citrus and from each other. Within Citrus, the separation into two subgenera, Citrus and Papeda, designated by Swingle, was clearly observed except for C. celebica and C. indica. Almost all the accessions belonging to subgenus Citrus fell into three clusters, each including 1 genotype that was considered to be a true species. Different phylogenetic relationships
were revealed with cpDNA data. Citrus genotypes were separated into subgenera Archicitrus and Metacitrus, as proposed by Tanaka, while the division of subgenera Citrus and Papeda disappeared. C. medica and C. indica were quite distant from other citrus as well from related genera. C. ichangensis appeared to be the ancestor of the mandarin cluster, including C. tachibana. Lemon and Palestine sweet lime were clustered into the Pummelo cluster led by C. latipes. C. aurantifolia was located in the Micrantha cluster. Furthermore, genetic origin was studied on 17 cultivated citrus genotypes by the same
molecular markers, and a hybrid origin was hypothesized for all the tested genotypes. The assumptions are discussed with respect
to previous studies; similar results were obtained for the origin of orange and grapefruit. Hybrids of citron and sour orange
were assumed for lemon, Palestine sweet lime, bergamot and Volkamer lemon, while a citron × mandarin hybrid was assumed for
Rangpur lime and Rough lemon. For Mexican lime our molecular data indicated C. micrantha to be the female parent and C. medica as the male one.
Received: 5 October 1999 / Accepted: 3 November 1999 相似文献