全文获取类型
收费全文 | 569篇 |
免费 | 21篇 |
专业分类
590篇 |
出版年
2022年 | 9篇 |
2021年 | 16篇 |
2020年 | 3篇 |
2019年 | 8篇 |
2018年 | 13篇 |
2017年 | 17篇 |
2016年 | 8篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 22篇 |
2012年 | 21篇 |
2011年 | 24篇 |
2010年 | 15篇 |
2009年 | 6篇 |
2008年 | 19篇 |
2007年 | 18篇 |
2006年 | 14篇 |
2005年 | 17篇 |
2004年 | 17篇 |
2003年 | 22篇 |
2002年 | 17篇 |
2001年 | 16篇 |
2000年 | 21篇 |
1999年 | 9篇 |
1995年 | 4篇 |
1992年 | 13篇 |
1991年 | 16篇 |
1990年 | 14篇 |
1989年 | 16篇 |
1988年 | 23篇 |
1987年 | 20篇 |
1986年 | 10篇 |
1985年 | 17篇 |
1984年 | 11篇 |
1983年 | 9篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1973年 | 7篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1970年 | 6篇 |
1969年 | 6篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 3篇 |
排序方式: 共有590条查询结果,搜索用时 10 毫秒
31.
32.
The evolution of pathogen virulence in natural populations has conventionally been considered as a result of selection caused by the interactions of the host with its pathogen(s). The host population, however, is generally embedded in complex trophic interactions with other populations in the community, in particular, intensive predation on the infected host can increase its mortality, and this can affect the course of virulence evolution. Reciprocally, in the long run, the evolution of virulence within an infected host can affect the patterns of population dynamics of a predator consuming the host (e.g. resulting in large amplitude oscillations, causing a severe drop in the population size, etc.). Surprisingly, neither the effect of predation on the evolution of virulence within a host, nor the influence of the evolution of virulence upon the consumer's dynamics has been addressed in the literature yet. In this paper, we consider a classical S-I ecoepidemiological model in which the infected host is consumed by a predator. We are particularly interested in the evolutionarily stable virulence of the pathogen in the model and its dependence upon ecologically relevant parameters. We show that predation can prominently shift the evolutionarily stable virulence towards more severe strains as compared to the same system without predation. We demonstrate that the evolution of virulence can result in a succession of dynamical regimes and can even lead to the extinction of the predator in the long run. The presence of a predator can indirectly affect the evolution within its prey since the evolutionarily stable virulence becomes a function of the prey growth rate, which would not be the case in a predator-free system. We find that the evolutionarily stable virulence largely depends on the carrying capacity K of the prey in a non-monotonous way. The model also predicts that in an eutrophic environment the shift of virulence towards evolutionarily stable benign strains can cause demographically stochastic evolutionary suicide, resulting in the extinction of both species, thus artificially maintaining severe strains of pathogen can enhance the persistence of both species. 相似文献
33.
RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex 总被引:1,自引:0,他引:1
Chang M Bellaoui M Zhang C Desai R Morozov P Delgado-Cruzata L Rothstein R Freyer GA Boone C Brown GW 《The EMBO journal》2005,24(11):2024-2033
SGS1 encodes a DNA helicase whose homologues in human cells include the BLM, WRN, and RECQ4 genes, mutations in which lead to cancer-predisposition syndromes. Clustering of synthetic genetic interactions identified by large-scale genetic network analysis revealed that the genetic interaction profile of the gene RMI1 (RecQ-mediated genome instability, also known as NCE4 and YPL024W) was highly similar to that of SGS1 and TOP3, suggesting a functional relationship between Rmi1 and the Sgs1/Top3 complex. We show that Rmi1 physically interacts with Sgs1 and Top3 and is a third member of this complex. Cells lacking RMI1 activate the Rad53 checkpoint kinase, undergo a mitotic delay, and display increased relocalization of the recombination repair protein Rad52, indicating the presence of spontaneous DNA damage. Consistent with a role for RMI1 in maintaining genome integrity, rmi1Delta cells exhibit increased recombination frequency and increased frequency of gross chromosomal rearrangements. In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response. 相似文献
34.
Cell thermosensitivity of Escherichia coli B/r increases with the cooling rise, especially in hypertonic conditions after heating at 50, 55 and 60 degrees C. A certain relationship is suggested between the observed phenomenon and the osmotic homeostasis system of microorganisms under condition of thermogenic and tonic stress. 相似文献
35.
Bayne EH Rakitina DV Morozov SY Baulcombe DC 《The Plant journal : for cell and molecular biology》2005,44(3):471-482
RNA silencing in transgenic and virus-infected plants involves a mobile silencing signal that can move cell-to-cell and systemically through the plant. It is thought that this signal can influence long-distance movement of viruses because protein suppressors of silencing encoded in viral genomes are required for long-distance virus movement. However, until now, it was not known whether the mobile signal could also influence short-range virus movement between cells. Here, through random mutation analysis of the Potato Potexvirus X (PVX) silencing suppressor P25, we provide evidence that it does. All mutants that were defective for silencing suppression were also non-functional in viral cell-to-cell movement. However, we identified mutant P25 proteins that were functional as silencing suppressors but not as movement proteins and we conclude that suppression of silencing is not sufficient to allow virus movement between cells: there must be a second P25 function that is independent of silencing but also required for cell-to-cell movement. Consistent with this hypothesis, we identified two classes of suppressor-inactive P25 mutants. One class of these mutants is proposed to be functional for the accessory function because their failure to support PVX movement could be complemented by heterologous suppressors of silencing. The second class of P25 mutants is considered defective for both the suppressor and second functions because the heterologous silencing suppressors did not restore virus movement. It is possible, based on analyses of short interfering RNA accumulation, that P25 suppresses silencing by interfering with either assembly or function of the effector complexes of RNA silencing. 相似文献
36.
Morozov II Morozova GV Petin VG 《Radiatsionnaia biologiia, radioecologiia / Rossi?skaia akademiia nauk》2005,45(3):305-309
The influence of 60Co gamma-ray radiation of high intensity (85 Gy/min) on the viability of E. coli B/r and E. coli BS-1 bacteria, cultivated in salt buffer with the concentration about 10(8) cells/ml, was studied. It was determined that under the doses, which induce about 80% of death of the cells, the irradiated bacteria, just like the intact cells, die during the incubation processes, while under the doses induced the death of cells above 95%, the cells viability of the both strains increases and reaches the constant value by the byhend 2nd-5th days of incubation in these conditions. In the result of the differences of the reactions of the intact and irradiated with different doses of radiation microorganisms on the incubation during their postradiational period in the phosphate buffer we have the fact of the absence of the dependence of the effect from the dose, or the decreasing of the consequences of the radiation under the increasing of the dose of the radiation. The nature of this phenomenology while stays not understood. 相似文献
37.
Wahlroos Tony Susi Petri Solovyev Andrej Dorokhov Yurii Morozov Sergeyi Atabekov Josif Korpela Timo 《Molecular breeding : new strategies in plant improvement》2005,14(4):455-462
An approach that enables the increase of the quantity of a specific amino acid in crop plants is reported. Oleosin gene from Arabidopsis thaliana or 30K movement protein gene of Tobacco mosaic virus (TMV; genus Tobamovirus) were cloned under the control of napin or hybrid promoters, and in fusion to synthetic poly-histidine (poly-His) sequences for transformation into spring turnip rape (Brassica rapa subsp. oleifera; synonym to B. campestris). The most stable expression cassettes for the poly-His production prior to the plant transformation were selected by analyzing the protein expression in in vitro translation and in transient plant expression systems using GFP as marker. Expression of the poly-His-constructs in transgenic Brassica rapa plants was analyzed using dot and western blotting and PCR. The constructs were stably expressed in the third generation of the transgenic plant lines. Histidine content was measured from the seeds of the transgenic plants, and some plant lines had more than 20% increase in histidine content compared to wild type. The methodology may be widely applicable to increase the content of any amino acid in crop plants including those encoded by rare codons. 相似文献
38.
We identified five genes encoding components of the TOR signaling pathway within Aspergillus nidulans. Unlike the situation in Saccharomyces cerevisiae, there is only a single Tor kinase, as in plant and animal systems, and mutant phenotypes suggest that the TOR pathway plays only a minor role in regulating nitrogen metabolism. 相似文献
39.
Paape M Solovyev AG Erokhina TN Minina EA Schepetilnikov MV Lesemann DE Schiemann J Morozov SY Kellmann JW 《Molecular plant-microbe interactions : MPMI》2006,19(8):874-883
The Tomato spotted wilt virus (TSWV) encoded NSm movement protein facilitates cell-to-cell spread of the viral genome through structurally modified plasmodesmata. NSm has been utilized as bait in yeast two-hybrid interaction trap screenings. As a result, a protein of unknown function, called At-4/1, was isolated from an Arabidopsis thaliana GAL4 activation domain-tagged cDNA library. Using polyclonal antibodies against bacterially expressed At-4/1, Western blot analysis of protein extracts isolated from different plant species as well as genome database screenings showed that homologues of At-4/1 seemed to be encoded by many vascular plants. For subcellular localization studies, At-4/1 was fused to green fluorescent protein, and corresponding expression vectors were used in particle bombardment and agroinfiltration assays. Confocal laser scannings revealed that At-4/1 assembled in punctate spots at the cell periphery. The protein accumulated intracellularly in a polarized fashion, appearing in only one-half of a bombarded epidermal cell, and, moreover, moved from cell to cell, forming twin-structured bodies seemingly located at both orifices of the plasmodesmatal pore. In coexpression studies, At-4/1 colocalized with a plant virus movement protein TGBp3 known to reside in endoplasmic reticulum-derived membrane structures located in close vicinity to plasmodesmata. Thus, At-4/1 belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking. 相似文献
40.