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671.
672.
Marco Scocchi Christine Lüthy Pietro Decarli Giuseppina Mignogna Philipp Christen Renato Gennaro 《International journal of peptide research and therapeutics》2009,15(2):147-155
Bac7, a cathelicidin peptide of the proline-rich group, inactivates bacteria in a stereospecific manner by entering target
cells without any apparent membrane damage and by binding to as yet unknown intracellular targets. The present study was aimed
at detecting these putative intracellular interactors, which might mediate the antibacterial action of this peptide. By using
affinity resins functionalized with the N-terminal 1-35 fragment of Bac7, a single protein was specifically retained with
high affinity from Escherichia coli cytoplasmic protein lysates. This ligand was identified as the heat shock protein DnaK, the Hsp70 homolog in E. coli. The interaction between the peptide and the chaperone is stereospecific, given that a resin prepared with the all-
d enantiomer failed to retain the protein. In vitro, Bac7(1-35) formed a complex with DnaK with an affinity comparable to that
of other known high-affinity peptide ligands. In addition, at 10–100 μM concentration, the peptide inhibited the protein refolding
activity of the complete DnaK/DnaJ/GrpE/ATP molecular chaperone system in a dose-dependent manner. Despite these results,
the in vitro sensitivity to the peptide, under growth permitting conditions, of DnaK-deficient E. coli strains was not significantly affected compared to the wild-type strain. This suggests that, apart from DnaK, other vital
targets for the proline-rich AMPs are present in susceptible bacteria.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Marco Scocchi and Christine Lüthy contributed equally to this work. 相似文献
673.
Giuseppe Giallongo Roberto Pilot Christian Durante Gian Andrea Rizzi Raffaella Signorini Renato Bozio Armando Gennaro Gaetano Granozzi 《Plasmonics (Norwell, Mass.)》2011,6(4):725-733
Commercially available digital versatile discs (DVDs) contain a silver-coated spiral distribution of rectangular-shaped grooves
(AgDVD): for the first time, they have been used to produce surface-enhanced Raman scattering (SERS) substrates by electrochemical
deposition of silver nanoparticles (AgNPs@AgDVD). The overall procedure only requires cheap and widely available materials
and can be easily accomplished. Scanning electron microscopy images of AgNPs@AgDVD revealed that small AgNPs (average diameter
about 15 nm) are present within the valleys of AgDVD, whereas over the ridges, the AgNPs are bigger, more densely packed and
with a dendrite-like morphology somewhere. The SERS properties of these substrates have been studied in terms of the enhancement
factor (EF), point-to-point reproducibility and sample-to-sample repeatability. It turned out that high SERS EF and good reproducibility
requirements are both fulfilled. As for repeatability, remarkably better results than typical literature values have been
achieved. Such an easy&cheap preparation along with efficient SERS properties make DVD-derived SERS substrates very good candidates for the development
of convenient and disposable sensing platforms. 相似文献