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11.
Sara Bruschini Simona di Martino Maria Elena Pisanu Luigi Fattore Claudia De Vitis Valentina Laquintana Simonetta Buglioni Eugenio Tabbì Andrea Cerri Paolo Visca Gabriele Alessandrini Francesco Facciolo Christian Napoli Marcella Trombetta Antonio Santoro Anna Crescenzi Gennaro Ciliberto Rita Mancini 《Journal of cellular physiology》2020,235(3):1877-1887
12.
The Psp system of Mycobacterium tuberculosis integrates envelope stress‐sensing and envelope‐preserving functions 下载免费PDF全文
13.
Patrizia Di Gennaro Linda Veronique Kazandjian Francesco Mezzetti Guido Sello 《Archives of microbiology》2013,195(4):269-278
This work reports the preparation of two recombinant strains each containing two enzymatic activities mutually expressed through regulated systems for production of functionalized epoxides in one-pot reactions. One strain was Pseudomonas putida PaW340, containing the gene coding for styrene monooxygenase (SMO) from Pseudomonas fluorescens ST under the auto-inducing Ptou promoter and the TouR regulator of Pseudomonas sp. OX1 and the gene coding for naphthalene dihydrodiol dehydrogenase (NDDH) from P. fluorescens N3 under the Ptac promoter inducible by IPTG. The second strain was Escherichia coli JM109, in which the expression of SMO was under the control of the Pnah promoter and the NahR regulator of P. fluorescens N3 inducible by salicylate, while the gene expressing NDDH was under the control of the Plac promoter inducible by IPTG. SMO and NDDH activities were tested in bioconversion experiments using cinnamyl alcohol as reference substrate. The application that we selected is one example of the sequential use of the two enzymatic activities which require a temporal control of the expression of both genes. 相似文献
14.
Federico Fogolari Haritha Haridas Alessandra Corazza Paolo Viglino Davide Corà Michele Caselle Gennaro Esposito Luigi E Xodo 《BMC structural biology》2009,9(1):64-20
Background
Independent surveys of human gene promoter regions have demonstrated an overrepresentation of G3X n1G3X n2G3X n3G3 motifs which are known to be capable of forming intrastrand quadruple helix structures. In spite of the widely recognized importance of G-quadruplex structures in gene regulation and growing interest around this unusual DNA structure, there are at present only few such structures available in the Nucleic Acid Database. In the present work we generate by molecular modeling feasible G-quadruplex structures which may be useful for interpretation of experimental data. 相似文献15.
16.
Antonio Politano Vincenzo Formoso Gennaro Chiarello 《Plasmonics (Norwell, Mass.)》2013,8(4):1683-1690
The collective electronic excitations in thin Ag films deposited onto the Ni(111) surface were studied by high-resolution electron energy loss spectroscopy. A broad loss peak at 7.7 eV was assigned to the Ag multipole plasmon, in excellent agreement with calculations based on s-d polarization model. Ag multipole plasmon was excited only at grazing incidence. Furthermore, a strong dependence on the impinging energy exists. Multipole plasmon could be measured only for a very strict range of primary electron beam energies and it was excited by electrons scattered at a reflection plane located just underneath the jellium edge. Such mode was found to be dramatically more sensible to the state of the surface with respect to ordinary surface plasmon. Moreover, we report experimental evidence of interference effects in surface plasmon excitation. 相似文献
17.
Joao A. Ascensao Pratik Datta Baris Hancioglu Eduardo Sontag Maria L. Gennaro Oleg A. Igoshin 《PLoS computational biology》2016,12(2)
Understanding how dynamical responses of biological networks are constrained by underlying network topology is one of the fundamental goals of systems biology. Here we employ monotone systems theory to formulate a theorem stating necessary conditions for non-monotonic time-response of a biochemical network to a monotonic stimulus. We apply this theorem to analyze the non-monotonic dynamics of the σB-regulated glyoxylate shunt gene expression in Mycobacterium tuberculosis cells exposed to hypoxia. We first demonstrate that the known network structure is inconsistent with observed dynamics. To resolve this inconsistency we employ the formulated theorem, modeling simulations and optimization along with follow-up dynamic experimental measurements. We show a requirement for post-translational modulation of σB activity in order to reconcile the network dynamics with its topology. The results of this analysis make testable experimental predictions and demonstrate wider applicability of the developed methodology to a wide class of biological systems. 相似文献
18.
19.
Tuberculosis (TB) remains one of the most devastating infectious diseases and its eradication is still unattainable given the limitations of current technologies for diagnosis, treatment and prevention. The World Health Organization's goal to eliminate TB globally by 2050 remains an ongoing challenge as delayed diagnosis and misdiagnosis of TB continue to fuel the worldwide epidemic. Despite considerable improvements in diagnostics for the last few decades, a simple and effective point-of-care TB diagnostic test is yet not available. Here, we review the current assays used for TB diagnosis, and highlight the recent advances in nanotechnology and microfluidics that potentially enable new approaches for TB diagnosis in resource-constrained settings. 相似文献
20.
Rym Zitari-Chatti Noureddine Chatti Domenico Fulgione Immacolata Caiazza Gennaro Aprea Ali Elouaer Khaled Said Teresa Capriglione 《Genetica》2009,136(3):439-447
In this study we analysed mitochondrial DNA variation in Penaeus kerathurus prawns collected from seven locations along a transect across the Siculo–Tunisian region in order to verify if any population
structuring exists over a limited geographical scale and to delineate the putative transition zone with sufficient accuracy.
Partial DNA sequences of COI and 16S genes were analysed. In contrast to the highly conservative 16S gene, the COI sequences
exhibited sufficient diversity for population analysis. The COI gene revealed low levels of haplotype and nucleotide diversities.
The size of the annual landings of this commercial species suggests large population sizes. Hence, the low genetic diversity
detected in this study could indicate a possible reduction in effective population sizes in the past. We detected significant
genetic differentiation between eastern and western populations likely due to restricted gene flow across the Siculo–Tunisian
boundary. We discuss the different evolutionary forces that may have shaped the genetic variation and suggest that the genetic
divide is probably maintained by present-day dispersal limitation.
R. Zitari-Chatti and N. Chatti are contributed equally to the work. 相似文献