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971.
New insights into the history of domesticated and wild apricots and its contribution to Plum pox virus resistance
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![点击此处可从《Molecular ecology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Stéphane Decroocq Amandine Cornille David Tricon Sevda Babayeva Aurélie Chague Jean‐Philippe Eyquard Raul Karychev Svetlana Dolgikh Tatiana Kostritsyna Shuo Liu Weisheng Liu Wenjuan Geng Kang Liao Bayram M. Asma Zeynal Akparov Tatiana Giraud Véronique Decroocq 《Molecular ecology》2016,25(19):4712-4729
Studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. Apricot is an important fruit in the Northern hemisphere, where it is threatened by the Plum pox virus (PPV), causing the sharka disease. The histories of apricot domestication and of its resistance to sharka are however still poorly understood. We used 18 microsatellite markers to genotype a collection of 230 wild trees from Central Asia and 142 cultivated apricots as representatives of the worldwide cultivated apricot germplasm; we also performed experimental PPV inoculation tests. The genetic markers revealed highest levels of diversity in Central Asian and Chinese wild and cultivated apricots, confirming an origin in this region. In cultivated apricots, Chinese accessions were differentiated from more Western accessions, while cultivated apricots were differentiated from wild apricots. An approximate Bayesian approach indicated that apricots likely underwent two independent domestication events, with bottlenecks, from the same wild population. Central Asian native apricots exhibited genetic subdivision and high frequency of resistance to sharka. Altogether, our results contribute to the understanding of the domestication history of cultivated apricot and point to valuable genetic diversity in the extant genetic resources of wild apricots. 相似文献
972.
Yan Zhou Panpan Geng Yalin Liu Jinyuan Wu Hongtu Qiao Yuping Xie Na Yin Linlin Chen Xiaochen Lin Yang Liu Shan Yi Guangming Zhang Hongjun Li Maosheng Sun 《生物化学与生物物理学报:疾病的分子基础》2018,1864(1):60-68
Rotaviruses are double-stranded RNA viruses that are a major cause of viral diarrhea in infants. Examining virus–host cell interaction is important for elucidating mechanisms of virus proliferation in host cells. Viruses can create an environment that promotes their survival and self-proliferation by encoding miRNAs or miRNA-like molecules that target various host cell. However, it remains unclear whether RNA viruses encode viral miRNAs, and their regulation mechanisms are largely unknown. We previously performed deep sequencing analysis to investigate rotavirus-encoded miRNAs, and identified the small RNA molecule Chr17_1755, which we named RV-vsRNA1755. In our present study, we determined that RV-vsRNA1755 is encoded by the rotavirus NSP4 gene and that it targets the host cell IGF1R, which is part of the PI3K/Akt pathway. We further explored the biological characteristics and functions of RV-vsRNA1755.Our results suggest that rotavirus adapts to manipulate PI3K/Akt signaling at early phases of infection. RV-vsRNA1755 targets IGF1R, blockading the PI3K/Akt pathway and triggering autophagy, but it ultimately inhibits autophagy maturation. A mechanism through which rotavirus encodes a virus-like small RNA (RV-vsRNA1755) that triggers autophagy by targeting the host cell IGF1R gene was revealed. These data provide a theoretical basis for therapeutic drug screening targeting RV-vsRNA1755. 相似文献
973.
974.
The Gammaproteobacteria are widely and abundantly distributed in various environments, and they play important roles in the geochemical cycles of biogenic elements (e.g., C, N and S) in the ecosystems. Previous studies showed that Gammaproteobacteria dominate in saline and hypersaline lakes. However, little is known on how salinity influences gammaproteobacterial community composition and their ecological functions (i.e., organic carbon degradation). In this study, we investigated the gammaproteobacterial diversity and carbon utilization and their response to salinity in six saline/hypersaline lakes and one freshwater lake on the Qinghai–Tibetan Plateau (QTP). The results indicated that the gammaproteobacterial community composition was mainly influenced by the salinity of the studied QTP lakes. Salinity was also a key environmental factor influencing the carbon utilization ability of Gammaproteobacteria: within one genus (e.g., Halomonas, Pseudoalteromonas, Vibrio) the strains retrieved from low-salinity environments had stronger carbon utilization ability than their counterparts from high-salinity environments; within one genus, the strains isolated from lakes with different salinity shared similar carbon utilization preference, indicating that species belonging to the same genus may execute similar ecological functions in the environments regardless of salinity. 相似文献
975.
Zhi Lin Lu Zhang Di Zhang Guitao Huo Xiaobing Zhou Yan‐wei Yang Yan Huo Bo Li Xing‐chao Geng 《Journal of medical primatology》2018,47(2):132-135
This report describes a suppurative meningitis in a young cynomolgus. The animal had neutrophil aggregation in the subarachnoid space and hemorrhage in bilateral adrenal glands. Staphylococcus was identified by FISH in brain. To our knowledge, this is the first case of staphylococcal meningitis with Waterhouse‐Friderichsen syndrome in a cynomolgus monkey. 相似文献
976.
977.
978.
Investigation of the interactions between aptamer and misfolded proteins: From monomer and oligomer to fibril by single‐molecule force spectroscopy
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Yan Zheng Qing Wang Xiaohai Yang Zhiping Li Lei Gao Hua Zhang Wenyan Nie Xiuhua Geng Kemin Wang 《Journal of molecular recognition : JMR》2018,31(4)
Increasing knowledge on the understanding interactions of aptamer with misfolded proteins (including monomer, oligomer, and amyloid fibril) is crucial for development of aggregation inhibitors and diagnosis of amyloid diseases. Herein, the interactions of lysozyme monomer–, oligomer‐, and amyloid fibril–aptamer were investigated using single‐molecule force spectroscopy. The results revealed that the aptamer screened against lysozyme monomer could also bind to oligomer and amyloid fibril, in spite of the recognition at a lower binding probability. It may be attributed to the inherent structural differences of misfolded proteins and the flexible conformation of aptamer. In addition, dynamic force spectra showed that there were similar dissociation paths in the dissociation process of lysozyme monomer–, oligomer‐, and amyloid fibril–aptamer complexes. It showed that the dissociation only passed 1 energy barrier from the binding state to the detachment. However, the dynamic parameters suggested that the oligomer‐ and amyloid fibril–aptamer were more stable than lysozyme monomer–aptamer. The phenomena may result from the exposure of aptamer‐recognized sequences on the surface and the electrostatic interactions. This work demonstrated that single‐molecule force spectroscopy could be a powerful tool to study the binding behavior of the aptamer with misfolded proteins at single‐molecule level, providing abundant information for researches and comprehensive applications of aptamer probes in diagnosis of amyloid diseases. 相似文献
979.
有机物沟埋还田模式与花后灌水量对玉米光合特性和产量的影响 总被引:4,自引:0,他引:4
有机物沟埋还田与花后灌水配合对增加玉米田保水供水能力,提高玉米花后光合性能、实现节水增产有重要意义.本试验以郑单958为供试材料,设置有机物沟埋还田和花后灌水量两个因素,有机物沟埋还田包括不还田(M0)、秸秆单还田(M1)和牛粪秸秆混合还田(M2)3个还田类型,花后灌水量包括450 mm(W1)和325 mm(W2)2个水平,研究了其对玉米穗位叶光合性能、光系统Ⅱ(PSⅡ)效率和产量等的影响.结果表明:与秸秆单还田比较,牛粪秸秆混合还田有效提高了玉米花后光合能力和各器官的干物质积累量;与节水灌溉相比,正常灌溉加强了有机物还田对玉米光合能力的促进作用.牛粪秸秆混合还田与正常灌溉结合可显著提高玉米花后叶片的光合速率(Pn)、气孔导度(gs)和蒸腾速率(Tr),降低胞间CO2浓度(Ci);提高玉米花后叶片PSⅡ的最大光化学效率(φpo)和捕获的激子将电子传递到电子传递链中QA下游的电子受体的概率(Ψo);改善花后叶片光能利用率,维持花后叶片较高的光合性能;同时增加花后玉米各器官干物质的量,提高干物质总积累量和转运能力,有利于花后同化物对籽粒的分配,最终获得高产.节水灌溉降低了叶片的光合性能,造成产量的下降;但配合牛粪秸秆混合还田与不还田处理相比,水分利用效率、籽粒增长速率和增产效果均优于正常灌水.这表明牛粪秸秆混合还田与正常灌溉结合可有效提高玉米花后光合性能,增加干物质积累量,促进玉米增产;牛粪秸秆混合还田与节水灌溉结合一定程度上降低了因减少灌溉量造成的减产. 相似文献
980.
Xue Teng Weiwei Chen Zhihan Liu Tao Feng Hui Li Sheng Ding Yonggang Chen Ying Zhang Xianping Tang Deqin Geng 《Neurochemical research》2018,43(6):1200-1209
Necroptosis is a manner of caspase-independent cell death,which accounts for delayed ischemic cerebral injury, and can be used as a novel tool to expand the treatment time window in ischemic cerebral injury. Q-VD-OPH, a novel pan caspase inhibitor, has been identified as an inducer of necroptosis. In this study, we determined the optimal dose of Q-VD-OPH, which induces necroptosis in rats by the middle cerebral artery occlusion, followed by reperfusion. Furthermore, we report that the NLRP3 inflammasome is involved in necroptosis, with levels of NLRP3 inflammasome proteins as well as inflammatory cytokines, such as IL-1β, being elevated. We also demonstrated that NLRP3 was not only expressed in microglia and vascular endothelial cell, but also in neurons when necroptosis is induced with Q-VD-OPH. Inhibition of NLRP3 by glyburide strongly suppressed the expression of NLRP3 inflammasome proteins and IL-1β, and markedly reduced brain tissue damage. Our findings provide evidence that pretreatment with Q-VD-OPH suppresses apoptosis and induces necroptosis in the cerebral ischemia-reperfusion model. We also identified that the NLRP3 inflammasome plays an important role in neuronal necroptosis, and that NLRP3 inflammasome deficiency reduces brain tissue damage after cerebral ischemia-reperfusion injury in rats. 相似文献