全文获取类型
收费全文 | 17861篇 |
免费 | 1411篇 |
国内免费 | 1591篇 |
专业分类
20863篇 |
出版年
2024年 | 43篇 |
2023年 | 227篇 |
2022年 | 607篇 |
2021年 | 1004篇 |
2020年 | 660篇 |
2019年 | 794篇 |
2018年 | 762篇 |
2017年 | 522篇 |
2016年 | 745篇 |
2015年 | 1113篇 |
2014年 | 1331篇 |
2013年 | 1384篇 |
2012年 | 1661篇 |
2011年 | 1472篇 |
2010年 | 898篇 |
2009年 | 818篇 |
2008年 | 903篇 |
2007年 | 798篇 |
2006年 | 722篇 |
2005年 | 671篇 |
2004年 | 518篇 |
2003年 | 475篇 |
2002年 | 371篇 |
2001年 | 297篇 |
2000年 | 278篇 |
1999年 | 271篇 |
1998年 | 170篇 |
1997年 | 158篇 |
1996年 | 181篇 |
1995年 | 136篇 |
1994年 | 154篇 |
1993年 | 98篇 |
1992年 | 113篇 |
1991年 | 108篇 |
1990年 | 79篇 |
1989年 | 72篇 |
1988年 | 48篇 |
1987年 | 55篇 |
1986年 | 38篇 |
1985年 | 28篇 |
1984年 | 37篇 |
1983年 | 18篇 |
1982年 | 14篇 |
1981年 | 9篇 |
1979年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
62.
Kaitlin U Laverty Arttu Jolma Sara E Pour Hong Zheng Debashish Ray Quaid Morris Timothy
R Hughes 《Nucleic acids research》2022,50(19):e111
Modelling both primary sequence and secondary structure preferences for RNA binding proteins (RBPs) remains an ongoing challenge. Current models use varied RNA structure representations and can be difficult to interpret and evaluate. To address these issues, we present a universal RNA motif-finding/scanning strategy, termed PRIESSTESS (Predictive RBP-RNA InterpretablE Sequence-Structure moTif regrESSion), that can be applied to diverse RNA binding datasets. PRIESSTESS identifies dozens of enriched RNA sequence and/or structure motifs that are subsequently reduced to a set of core motifs by logistic regression with LASSO regularization. Importantly, these core motifs are easily visualized and interpreted, and provide a measure of RBP secondary structure specificity. We used PRIESSTESS to interrogate new HTR-SELEX data for 23 RBPs with diverse RNA binding modes and captured known primary sequence and secondary structure preferences for each. Moreover, when applying PRIESSTESS to 144 RBPs across 202 RNA binding datasets, 75% showed an RNA secondary structure preference but only 10% had a preference besides unpaired bases, suggesting that most RBPs simply recognize the accessibility of primary sequences. 相似文献
63.
人乳寡糖(Human milk oligosaccharides,HMO)是母乳中重要的免疫活性成分,对婴幼儿健康起到显著促进作用。2’-岩藻糖基乳糖(2’-FL)是HMO的主要组分,极具应用价值,3-岩藻糖基乳糖(3-FL)与2’-FL的合成途径相似,两者的研究具有相互借鉴意义,近年来针对它们的研究取得了较多进展。以微生物细胞工厂为核心理念的新型生物合成途径有望将2’-FL和3-FL产业化,未来将对乳制品行业产生重要的影响。文中综述了生物技术制备2’-FL和3-FL的最新研究进展,并对未来发展趋势进行了展望。 相似文献
64.
Li Bai Wang Lu Shan Tang Huixin Tang Manman Xu Chen Liang Sujun Zheng Shuang Liu Ming Kong Zhongping Duan Yu Chen 《Cell death & disease》2022,13(9)
We previously documented that M2-like macrophages exert a hepatoprotective effect in acute-on-chronic liver failure (ACLF) by inhibiting necroptosis signalling. Nevertheless, the molecular mechanism behind this hepatoprotection still needs to be further dissected. Galectin-3 (GAL3) has been reported to be critically involved in the pathogenesis of multiple liver diseases, whereas the potential role of GAL3 in ACLF remains to be explored. Herein, we hypothesised that GAL3 plays a pivotal role in the hepatoprotection conferred by M2-like macrophages in ACLF by inhibiting necroptosis. To test this hypothesis, we first assessed the expression of GAL3 in control and fibrotic mice with or without acute insult. Second, loss- and gain-of-function experiments of GAL3 were performed. Third, the correlation between GAL3 and M2-like macrophage activation was analysed, and the potential role of GAL3 in M2-like macrophage-conferred hepatoprotection was confirmed. Finally, the molecular mechanism underlying GAL3-mediated hepatoprotection was dissected. GAL3 was found to be obviously upregulated in fibrotic mice with or without acute insult but not in acutely injured mice. Depletion of GAL3 aggravated hepatic damage in fibrotic mice upon insult. Conversely, adoptive transfer of GAL3 provided normal mice enhanced resistance against acute insult. The expression of GAL3 is closely correlated with M2-like macrophage activation. Through adoptive transfer and depletion experiments, M2-like macrophages were verified to act as a major source of GAL3. Importantly, GAL3 was confirmed to hold a pivotal place in the hepatoprotection conferred by M2-like macrophages through loss- and gain-of-function experiments. Unexpectedly, the depletion and adoptive transfer of GAL3 resulted in significant differences in the expression levels of pyroptosis but not necroptosis signalling molecules. Taken together, GAL3 plays a pivotal role in the hepatoprotection conferred by M2-like macrophages in ACLF by inhibiting pyroptosis but not necroptosis signalling. Our findings provide novel insights into the pathogenesis and therapy of ACLF.Subject terms: Inflammasome, Necroptosis 相似文献
65.
66.
Rui Cheng Xiaoman Zheng Yingmei Wang Xing Ma Xin Liu Wenjun Xu Mengyun Wang Yuanpeng Gao Xupeng Xing Chuan Zhou Hongzheng Sun Zekun Guo Fusheng Quan Jun Liu Song Hua Yongsheng Wang Yong Zhang Xu Liu 《中国科学:生命科学英文版》2022,65(11):2257-2268
Animal cloning can be achieved by somatic cell nuclear transfer(SCNT), but the resulting live birth rate is relatively low. We previously improved the efficiency of bovine SCNT by exogenous melatonin treatment or by overexpression of lysine-specific demethylase 4D(KDM4D) and 4E(KDM4E). In this study, we revealed abundant alternative splicing(AS) transitions during fertilization and embryonic genome activation, and demonstrated abnormal AS in bovine SCNT embryos compared with in vitro fertilized ... 相似文献
67.
68.
69.
Jia-Chen Hu Chen-Xi Zheng Bing-Dong Sui Wen-Jia Liu Yan Jin 《World journal of stem cells》2022,14(5):318-329
Poor healing of cutaneous wounds is a common medical problem in the field of traumatology. Due to the intricate pathophysiological processes of wound healing, the use of conventional treatment methods, such as chemical molecule drugs and traditional dressings, have been unable to achieve satisfactory outcomes. Within recent years, explicit evidence suggests that mesenchymal stem cells (MSCs) have great therapeutic potentials on skin wound healing and regeneration. However, the direct application of MSCs still faces many challenges and difficulties. Intriguingly, exosomes as cell-secreted granular vesicles with a lipid bilayer membrane structure and containing specific components from the source cells may emerge to be excellent substitutes for MSCs. Exosomes derived from MSCs (MSC-exosomes) have been demonstrated to be beneficial for cutaneous wound healing and accelerate the process through a variety of mechanisms. These mechanisms include alleviating inflammation, promoting vascularization, and promoting proliferation and migration of epithelial cells and fibroblasts. Therefore, the application of MSC-exosomes may be a promising alternative to cell therapy in the treatment of cutaneous wounds and could promote wound healing through multiple mechanisms simultaneously. This review will provide an overview of the role and the mechanisms of MSC-derived exosomes in cutaneous wound healing, and elaborate the potentials and future perspectives of MSC-exosomes application in clinical practice. 相似文献
70.
Yung-Tsi Bolon Bindu Joseph Steven B Cannon Michelle A Graham Brian W Diers Andrew D Farmer Gregory D May Gary J Muehlbauer James E Specht Zheng Jin Tu Nathan Weeks Wayne W Xu Randy C Shoemaker Carroll P Vance 《BMC plant biology》2010,10(1):1-24