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71.
We describe an emigration of the Neotropical army ant Eciton mexicanum where the head of the emigration column was separated in time from previous raid column activity, and the emigration was not connected to the new bivouac site by a column of workers. Over 12 h elapsed between raid activity and the onset of emigration, suggesting the emigration followed a long-lasting pheromone trail. We suggest the bivouac site had been selected the night before the emigration by foraging workers. 相似文献
72.
A characteristic of neurological disorders is the loss of critical populations of cells that the body is unable to replace,thus there has been much interest in identifying methods of generating clinically relevant numbers of cells to replace those that have been damaged or lost.The process of neural direct conversion,in which cells of one lineage are converted into cells of a neural lineage without first inducing pluripotency,shows great potential,with evidence of the generation of a range of functional neural cell types both in vitro and in vivo,through viral and non-viral delivery of exogenous factors,as well as chemical induction methods.Induced neural cells have been proposed as an attractive alternative to neural cells derived from embryonic or induced pluripotent stem cells,with prospective roles in the investigation of neurological disorders,including neurodegenerative disease modelling,drug screening,and cellular replacement for regenerative medicine applications,however further investigations into improving the efficacy and safety of these methods need to be performed before neural direct conversion becomes a clinically viable option.In this review,we describe the generation of diverse neural cell types via direct conversion of somatic cells,with comparison against stem cell-based approaches,as well as discussion of their potential research and clinical applications. 相似文献
73.
The roles of MAPKs in disease 总被引:2,自引:0,他引:2
Lawrence MC Jivan A Shao C Duan L Goad D Zaganjor E Osborne J McGlynn K Stippec S Earnest S Chen W Cobb MH 《Cell research》2008,18(4):436-442
74.
The progress of replication forks is often threatened in vivo, both by DNA damage and by proteins bound to the template. Blocked forks must somehow be restarted, and the original blockage cleared, in order to complete genome duplication, implying that blocked fork processing may be critical for genome stability. One possible pathway that might allow processing and restart of blocked forks, replication fork reversal, involves the unwinding of blocked forks to form four-stranded structures resembling Holliday junctions. This concept has gained increasing popularity recently based on the ability of such processing to explain many genetic observations, the detection of unwound fork structures in vivo and the identification of enzymes that have the capacity to catalyse fork regression in vitro. Here, we discuss the contexts in which fork regression might occur, the factors that may promote such a reaction and the possible roles of replication fork unwinding in normal DNA metabolism. 相似文献
75.
Olivia Bustos Saijal Naik Gayle Ayers Claudio Casola Maria A. Perez-Lamigueiro Paul T. Chippindale Ellen J. Pritham Elena de la Casa-Esperón 《Gene》2009
Genes of the Schlafen family, first discovered in mouse, are expressed in hematopoietic cells and are involved in immune processes. Previous results showed that they are candidate genes for two major phenomena: meiotic drive and embryonic lethality (DDK syndrome). However, these genes remain poorly understood, mostly due to the limitations imposed by their similarity, close location and the potential functional redundancy of the gene family members. 相似文献
76.
Genetic diversity and population structure in Malus sieversii, a wild progenitor species of domesticated apple 总被引:1,自引:0,他引:1
Christopher M. Richards Gayle M. Volk Ann A. Reilley Adam D. Henk Dale R. Lockwood Patrick A. Reeves Philip L. Forsline 《Tree Genetics & Genomes》2009,5(2):339-347
Malus sieversii (Lebed.) M. Roem. is a wild progenitor species of the domesticated apple. It is found across a mountainous region of central
Asia and has been the focus of several collection expeditions by the USDA-ARS-National Plant Germplasm System. This study
used microsatellite variation at seven loci to estimate diversity and differentiation within M. sieversii using several complimentary approaches. Multilocus genotypes were amplified from 949 individuals representing seedling trees
from 88 half-sib families from eight M. sieversii populations collected in Kazakhstan. Apportioning of genetic variation was estimated at both the family and site level. Analyses
using a hierarchical model to estimate F
st showed that differentiation among individual families is more than three times greater than differentiation among sites.
In addition, average gene diversity and allelic richness varied significantly among sites. A rendering of a genetic network
among all sites showed that differentiation is largely congruent with geographical location. In addition, nonhierarchical
Bayesian assignment methods were used to infer genetic clusters across the collection area. We detected four genetic clusters
in the data set. The quality of these assignments was evaluated over multiple Markov Chain Monte Carlo runs using both posterior
likelihood and stability of the assignments. The spatial pattern of genetic assignments among the eight collection sites shows
two broadly distributed and two narrowly distributed clusters. These data indicate that the southwestern collection sites
are more admixed and more diverse than the northern sites. 相似文献
77.
Carla P. Catterall Jarrad A. Cousin Scott Piper Gayle Johnson 《Diversity & distributions》2010,16(4):559-570
Aim Urbanization and deforestation are important drivers of biodiversity change. However, long‐term changes in faunal communities within urbanizing regions are poorly understood. We investigated how well observed community changes in both space and time agree with expectations based on current paradigms in urban ecology. Location Greater Brisbane region, Australia. Methods We compared bird assemblages in two time‐periods 15 years apart, at multiple sites in remnant forest and residential suburbs across an urbanizing landscape. Differences in assemblage composition, species abundances and functional groupings were assessed within and between habitats. Results Compared with forest, suburbs in both time‐periods had over twice the total bird abundance, a different species composition, greater between‐site community similarity, a greater proportion of non‐native species and greater dominance by large‐bodied species. These differences corresponded with changes in sites whose habitat was converted from forest to suburb. Between time‐periods, abundances of 58% of suburban species changed significantly compared with those of 11% in forest. Increaser species outnumbered decreasers in suburbs, with the reverse in forest. Abundance of small‐bodied birds decreased 70% in suburbs and 20% in forest. Broad‐spectrum competitors and nest predators were common among suburban increasers. Among invasive species, the number of increasers was counterbalanced by decreasers. Both site‐scale species richness and between‐site community similarity increased to a small extent in both habitats. Main conclusions Species composition and ecological function of suburban bird communities were very dynamic. Suburban assemblages were neither a subset of forest species nor an increasingly non‐native compilation. Communities in large forest patches were comparatively stable. The notion of habitat‐specific species turnover better characterizes the nature of most changes than either species decline or homogenization, even though both of these were evident. There is considerable scope for careful urban planning, focused on both among‐ and within‐habitat variety, to sustain bird diversity in urbanizing landscapes. 相似文献
78.
Benjamin Linard Ingo Ebersberger Shawn E McGlynn Natasha Glover Tomohiro Mochizuki Mateus Patricio Odile Lecompte Yannis Nevers Paul D Thomas Toni Gabaldn Erik Sonnhammer Christophe Dessimoz Ikuo Uchiyama QFO Consortium 《Molecular biology and evolution》2021,38(8):3033
Accurate determination of the evolutionary relationships between genes is a foundational challenge in biology. Homology—evolutionary relatedness—is in many cases readily determined based on sequence similarity analysis. By contrast, whether or not two genes directly descended from a common ancestor by a speciation event (orthologs) or duplication event (paralogs) is more challenging, yet provides critical information on the history of a gene. Since 2009, this task has been the focus of the Quest for Orthologs (QFO) Consortium. The sixth QFO meeting took place in Okazaki, Japan in conjunction with the 67th National Institute for Basic Biology conference. Here, we report recent advances, applications, and oncoming challenges that were discussed during the conference. Steady progress has been made toward standardization and scalability of new and existing tools. A feature of the conference was the presentation of a panel of accessible tools for phylogenetic profiling and several developments to bring orthology beyond the gene unit—from domains to networks. This meeting brought into light several challenges to come: leveraging orthology computations to get the most of the incoming avalanche of genomic data, integrating orthology from domain to biological network levels, building better gene models, and adapting orthology approaches to the broad evolutionary and genomic diversity recognized in different forms of life and viruses. 相似文献
79.
80.
Genome duplication requires not only unwinding of the template but also the displacement of proteins bound to the template, a function performed by replicative helicases located at the fork. However, accessory helicases are also needed since the replicative helicase stalls occasionally at nucleoprotein complexes. In Escherichia coli, the primary and accessory helicases DnaB and Rep translocate along the lagging and leading strand templates, respectively, interact physically and also display cooperativity in the unwinding of model forked DNA substrates. We demonstrate here that this cooperativity is displayed only by Rep and not by other tested helicases. ssDNA must be exposed on the leading strand template to elicit this cooperativity, indicating that forks blocked at protein-DNA complexes contain ssDNA ahead of the leading strand polymerase. However, stable Rep-DnaB complexes can form on linear as well as branched DNA, indicating that Rep has the capacity to interact with ssDNA on either the leading or the lagging strand template at forks. Inhibition of Rep binding to the lagging strand template by competition with SSB might therefore be critical in targeting accessory helicases to the leading strand template, indicating an important role for replisome architecture in promoting accessory helicase function at blocked replisomes. 相似文献