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Gayathri Gopalakrishnan MARIA Cristina Negri William Salas 《Global Change Biology Bioenergy》2012,4(6):739-750
Current research on the environmental sustainability of bioenergy has largely focused on the potential of bioenergy crops to sequester carbon and mitigate greenhouse gas emissions and possible impacts on water quality and quantity. A key assumption in these studies is that bioenergy crops will be grown in a manner similar to current agricultural crops such as corn and hence would affect the environment similarly. In this study, we investigate an alternative cropping system where bioenergy crops are grown in buffer strips adjacent to current agricultural crops such that nutrients present in runoff and leachate from the traditional row‐crops are reused by the bioenergy crops (switchgrass, miscanthus and native prairie grasses) in the buffer strips, thus providing environmental services and meeting economic needs of farmers. The process‐based biogeochemical model Denitrification‐Decomposition (DNDC) was used to simulate crop yield, nitrous oxide production and nitrate concentrations in leachate for a typical agricultural field in Illinois. Model parameters have been developed for the first time for miscanthus and switchgrass in DNDC. Results from model simulations indicated that growing bioenergy crops in buffer strips mitigated nutrient runoff, reduced nitrate concentrations in leachate by 60–70% and resulted in a reduction of 50–90% in nitrous oxide emissions compared with traditional cropping systems. While all the bioenergy crop buffers had significant positive environmental benefits, switchgrass performed the best with respect to minimizing nutrient runoff and nitrous oxide emissions, while miscanthus had the highest yield. Overall, our model results indicated that the bioenergy crops grown in these buffer strips achieved yields that are comparable to those obtained for traditional agricultural systems while simultaneously providing environmental services and could be used to design sustainable agricultural landscapes. 相似文献
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The major resolution of the study was to develop a dynamic form of natural biopolymer material to improve the wound healing by inhibition of biofilm formation on the surface. The extraction of collagen was effectively prepared from Scomberomorus lineolatus fish skin. Lyophilized collagen sheet was liquefied in 0.5M acetic acid to form acidic solubilized collagen (ASC) for further analysis. Physicochemical characterization of ASC was performed by various techniques using a standard protocol. The yield of ASC form S.lineolatus is higher (21.5%) than the previous reported studies. The effect of collagen solubility is gradually decreases with increasing concentration of NaCl and collagen is mostly soluble in acidic pH conditions. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of ASC contains α chain composition of α1 and α2 subunits and was characterized as type I collagen. Ultraviolet absorption was regulated as the appropriate wavelength to optimize the collagen. Fourier-transform infrared spectroscopy and X-ray diffraction confirmed that the isolated collagen is a triple-helical structure. The biofilm formation of Pseudomonas aeruginosa was significantly reduced by collagen incorporated with isolated 3,5,7-trihydroxyflavone (collagen-TF) sheet up to 70%. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay executed on fibroblast cell lines (L929) shows that the collagen-TF sheet was 100% compatible to enrich the cell adhesion and proliferation. The current study was the first report to extract, purify, and characterize ASC from S. lineolatus fish skin and characterize as type I collagen. Based on the result, we design the natural biodegradable collagen loaded with TF compound (collagen-TF) for antibiofilm properties. Compared with different sources of polymer, fish skin collagen is more effective and can be used as a biopolymer sheet for wound healing, food, drug delivery, tissue engineering, and pharmaceutical application. 相似文献
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Amirtharaj Francis Balaraju Sunitha Kandavalli Vinodh Kiran Polavarapu Shiva Krishna Katkam Sailesh Modi M. M. Srinivas Bharath Narayanappa Gayathri Atchayaram Nalini Kumarasamy Thangaraj 《PloS one》2014,9(7)
TCAP encoded telethonin is a 19 kDa protein, which plays an important role in anchoring titin in Z disc of the sarcomere, and is known to cause LGMD2G, a rare muscle disorder characterised by proximal and distal lower limb weakness, calf hypertrophy and loss of ambulation. A total of 300 individuals with ARLGMD were recruited for this study. Among these we identified 8 clinically well characterised LGMD2G cases from 7 unrelated Dravidian families. Clinical examination revealed predominantly proximo - distal form of weakness, scapular winging, muscle atrophy, calf hypertrophy and foot drop, immunoblot showed either complete absence or severe reduction of telethonin. Genetic analysis revealed a novel nonsense homozygous mutation c.32C>A, p.(Ser11*) in three patients of a consanguineous family and an 8 bp homozygous duplication c.26_33dupAGGTGTCG, p.(Arg12fs31*) in another patient. Both mutations possibly lead to truncated protein or nonsense mediated decay. We could not find any functionally significant TCAP mutation in the remaining 6 samples, except for two other polymorphisms, c.453A>C, p.( = ) and c.-178G>T, which were found in cases and controls. This is the first report from India to demonstrate TCAP association with LGMD2G. 相似文献
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