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91.
Girish Kumar V Surendranathan KP Gayathri Devi DR Belwadi MR 《Indian journal of experimental biology》2005,43(2):187-191
Dietary onion and garlic caused an increase in the level of liver and plasma total lipids in Coturnix coturnix japonica. This increase could be due to the effect of an increased feed intake, bile production, digestion and absorption that in turn caused an increased utilisation of dietary fat, increased transfer of dietary lipids to the liver and/or due to increased lipogenesis as such. However, there was no increase in the muscle lipid content. This effect in the muscle could be due to inhibitory effect of onion or garlic on lipoprotein lipase activity. Further, the changes in the tissue total lipid level in the control group due to change in age and sex were also observed. 相似文献
92.
93.
Eaazhisai K Jayalakshmi R Gayathri P Anand RP Sumathy K Balaram H Murthy MR 《Journal of molecular biology》2004,335(5):1251-1264
In the absence of the de novo purine nucleotide biosynthetic pathway in parasitic protozoa, purine salvage is of primary importance for parasite survival. Enzymes of the salvage pathway are, therefore, good targets for anti-parasitic drugs. Adenylosuccinate synthetase (AdSS), catalysing the first committed step in the synthesis of AMP from IMP, is a potential target for anti-protozoal chemotherapy. We report here the crystal structure of adenylosuccinate synthetase from the malaria parasite, Plasmodium falciparum, complexed to 6-phosphoryl IMP, GDP, Mg2+ and the aspartate analogue, hadacidin at 2 A resolution. The overall architecture of P. falciparum AdSS (PfAdSS) is similar to the known structures from Escherichia coli, mouse and plants. Differences in substrate interactions seen in this structure provide a plausible explanation for the kinetic differences between PfAdSS and the enzyme from other species. Additional hydrogen bonding interactions of the protein with GDP may account for the ordered binding of substrates to the enzyme. The dimer interface of PfAdSS is also different, with a pronounced excess of positively charged residues. Differences highlighted here provide a basis for the design of species-specific inhibitors of the enzyme. 相似文献
94.
Gurumurthy P Gayathri TN Bhagavathy S Venkatesan P 《Indian journal of experimental biology》2004,42(1):68-73
A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C. 相似文献
95.
Srinivas KS Chandrasekar G Srivastava R Puvanakrishnan R 《Journal of biochemical and biophysical methods》2004,60(1):23-27
In this paper, we describe a method to obtain a relatively pure mitochondrial and microsomal fractions by subcellular fractionation of human hepatoma cell line C3A using sucrose as the hypoosmotic medium. The cells were subjected to osmotic stress with sucrose and homogenized. Osmolarity was then restored to the cells and the organelles were separated by density gradient centrifugation. The protein profiles were examined by SDS-PAGE and the purity was analysed by marker enzymes and Western blotting. Our results indicate a good separation of mitochondrial and microsomal fractions from human hepatoma C3A cells. 相似文献
96.
Tomato (Lycopersicon esculentum Mill. cv. PKM 1) plants growing under field conditions were exposed for 15 d to solar radiation
with UV-B component (280 - 320 nm) enhanced to 6.3 kJ m-2 d-1. This simulated a 15% stratospheric ozone depletion over Madurai
(9° 50′ N latitude). Lipid peroxidation in the leaves of UV-B treated plants was 32% higher compared to the control. Superoxide
dismutase (SOD) and catalase activities registered parallel promotion by 126 and 50 %, respectively, in the UV-B treated plants.
Further, the contents of total phenols and anthocyanins in the leaves have also been enhanced by 40 and 156%, respectively.
On the contrary, polyphenol oxidase activity demonstrated a 58 % inhibition in the leaves of UV-B treated plants. While anthocyanins
and phenols are proposed to act as antioxidants, the reduction in polyphenol oxidase activity may maintain the turnover of
phenols in the UV-B treated plants.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
97.
98.
Using public surveys to reliably and rapidly estimate the distributions of multiple invasive species on the Andaman archipelago 下载免费PDF全文
Nitya Prakash Mohanty Anand Sachin Gayathri Selvaraj Karthikeyan Vasudevan 《Biotropica》2018,50(2):197-201
To effectively manage multiple biological invasions, information on their distributions must be generated rapidly and over large spatial scales. Using public surveys in a false‐positive occupancy framework, we reliably estimate the distributions of three synanthropic invasive species on the Andaman Islands. 相似文献
99.
Bandopadhyay Debashree Manish Kumar Thottethodi Subrahmanya Keshava Prasad Archana Natarajan Rita Christopher Atchayaram Nalini Parayil Sankaran Bindu Narayanappa Gayathri Muchukunte Mukunda Srinivas Bharath 《Journal of neurochemistry》2018,145(4):323-341
100.
The regulation of plasma membrane (PM)-localized transmembrane protein/receptor trafficking has critical implications for cell signaling, metabolism and survival. In this study, we investigated the role of BECN1 (Beclin 1) in the degradative trafficking of PM-associated APP (amyloid β precursor protein), whose metabolism to amyloid-β, an essential event in Alzheimer disease, is dependent on divergent PM trafficking pathways. We report a novel interaction between PM-associated APP and BECN1 that recruits macroautophagy/endosomal regulatory proteins PIK3C3 and UVRAG. We found that BECN1 promotes surface APP internalization and sorting predominantly to endosomes and endolysosomes. BECN1 also promotes the targeting of a smaller fraction of internalized APP to LC3-positive phagophores, suggesting a role for BECN1-dependent PM macroautophagy in APP degradation. Furthermore, BECN1 facilitates lysosomal degradation of surface APP and reduces the secretion of APP metabolites (soluble ectodomains, sAPP). The association between APP and BECN1 is dependent on the evolutionarily conserved domain (ECD) of BECN1 (amino acids 267–337). Deletion of a BECN1 ECD subregion (amino acids 285–299) did not impair BECN1- PIK3C3 interaction, PtdIns3K function or macroautophagy, but was sufficient to impair the APP-BECN1 interaction and BECN1's effects on surface APP internalization and degradation, resulting in increased secretion of sAPPs. Interestingly, both the BECN1-APP association and BECN1-dependent APP endocytosis and degradative trafficking were negatively regulated by active AKT. Our results further implicate phosphorylation of the BECN1 Ser295 residue in the inhibition of APP degradation by AKT. Our studies reveal a novel function for BECN1 in the sorting of a plasma membrane protein for endolysosomal and macroautophagic degradation. 相似文献