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41.
T. B. Smith J. Blondeau R. S. Nemeth S. J. Pittman J. M. Calnan E. Kadison J. Gass 《Coral reefs (Online)》2010,29(2):289-308
Coral reef banks may form an important component of mesophotic coral ecosystems (MCEs) in the Caribbean, but remain poorly
explored relative to shallower reefs and mesophotic habitats on slopes and walls. Consequently, the processes structuring
mesophotic coral reef communities are not well understood, particularly the role of disturbance. A large and regionally important
mesophotic system, the Hind Bank Marine Conservation District (MCD), St. Thomas, USVI, was systematically surveyed. Data were
used to construct a comprehensive benthic habitat map for the MCD, describe the abiotic and biotic components of the benthos
among habitats, and investigate patterns of coral health among habitats. Two-thirds of the MCD (23.6 km2) was found to be dense coral reef (Coral Cover = 24.1%) dominated by the Montastraea annularis species complex. Coral reef ecosystems were topographically complex, but could be classified into distinct habitat types,
including high coral banks (35.8% of the MCD) and two large novel coral reef habitat types corresponding to an extremely flat
basin (18%) and a highly rugose hillock basin (6.5%), containing thousands of coral knolls (2–10 m high). An extreme disease
event with undescribed signs of mortality occurred on 47% of coral reefs and reached a high prevalence in affected areas (42.4% ± 6.3
SE, N = 26). The disease was significantly clustered in the basin habitats of the western MCD (global Moran’s I = 0.32, P < 0.01). Observations of the spatial pattern suggested that the driver was specific to the basin habitats and may have been
caused by a coherent abiotic event. 相似文献
42.
The molecular mechanisms by which plants sense their micronutrient status, and adapt to their environment in order to ensure a sufficient micronutrient supply, are poorly understood. Zinc is an essential micronutrient for all living organisms. when facing a shortage in zinc supply, plants adapt by enhancing the zinc uptake capacity. The molecular regulators controlling this adaptation were recently identified. in this mini-review, we highlight recent progress in understanding the adaptation to zinc deficiency in plants and discuss the future challenges to fully unravel its molecular basis.Key words: adaptation, zinc deficiency, biofortification, molecular regulators, plant nutritionIn an increasingly populated world, agricultural production is an essential element of social development. Agriculture is the primary source of all nutrients required for human life, and nutrient sufficiency is the basis for good health and welfare of the human population.1 Soils with zinc deficiency are widespread in the world, affecting large areas of cultivated soils in India, Turkey, China, Brazil and Australia,2,3 making zinc the most common crop micronutrient deficiency.4 In addition, risk of inadequate zinc diet and zinc malnutrition are estimated to affect one-third of the global human population, i.e., around two billion people.5 Most affected are people living in developing countries, where diets are rich in cereal-based foods. Cereal grains are rich in phytate, which is a potent anti-nutrient, limiting micronutrient bioavailability.6 Zinc deficiency in crop production can be easily ameliorated through zinc fertilization, making agronomic biofortification an important strategy,3 however in the poorer regions, the required infrastructure to provide a reliable supply of zinc fertilizers of sufficient quality, is often not available. In those situations, biofortified crops, in which the zinc status of crops is genetically improved by selective breeding or via biotechnology, offer a rural-based intervention that will more likely reach the population.7 Different traits can be targeted to developing such improved crops, such as plant zinc deficiency tolerance, zinc use efficiency and the accumulation of zinc in edible parts. However, insufficient knowledge on the molecular mechanisms and the regulation of the zinc homeostasis network in plants is a serious bottleneck when pursuing zinc biofortification. 相似文献
43.
Oligomerization, chaperone activity, and nuclear localization of p26, a small heat shock protein from Artemia franciscana 总被引:5,自引:0,他引:5
Sun Y Mansour M Crack JA Gass GL MacRae TH 《The Journal of biological chemistry》2004,279(38):39999-40006
Artemia franciscana embryos undergo encystment, developmental arrest and diapause, the last characterized by profound metabolic dormancy and extreme stress resistance. Encysted embryos contain an abundant small heat shock protein termed p26, a molecular chaperone that undoubtedly has an important role in development. To understand better the role of p26 in Artemia embryos, the structural and functional characteristics of full-length and truncated p26 expressed in Escherichia coli and COS-1 cells were determined. p26 chaperone activity declined with increasing truncation of the protein, and those deletions with the greatest adverse effect on protection of citrate synthase during thermal stress had the most influence on oligomerization. When produced in either prokaryotic or eukaryotic cells the p26 alpha-crystallin domain consisting of amino acid residues 61-152 existed predominantly as monomers, and p26 variants lacking the amino-terminal domain but with intact carboxyl-terminal extensions were mainly monomers and dimers. The amino terminus was, therefore, required for efficient dimer formation. Assembly of higher order oligomers was enhanced by the carboxyl-terminal extension, although removing the 10 carboxyl-terminal residues had relatively little effect on oligomerization and chaperoning. Full-length and carboxyl-terminal truncated p26 resided in the cytoplasm of transfected COS-1 cells; however, variants missing the complete amino-terminal domain and existing predominantly as monomers/dimers entered the nuclei. A mechanism whereby oligomer disassembly assisted entry of p26 into nuclei was suggested, this of importance because p26 translocates into Artemia embryo nuclei during development and stress. However, when examined in Artemia, the p26 oligomer size was unchanged under conditions that allowed movement into nuclei, suggesting a process more complex than just oligomer dissociation. 相似文献
44.
Suarez RK Gass CL 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2002,133(2):335-343
Because of their small size and expensive mode of flight, hummingbirds display some of the highest known mass-specific rates of aerobic metabolism among vertebrates. High enzymatic flux capacities through pathways of carbohydrate and long-chain fatty acid oxidation indicate that either substrate can fuel flight. Although hummingbirds are known to rely on fat to fuel migratory flight, short foraging bouts are fueled by the oxidation of carbohydrate, not fat. This allows birds refueling at meadows during migration to deposit fat at higher rates and avoids the energetic inefficiency that results from synthesizing fat from dietary sugar, and then breaking down the fat to fuel foraging flight. On cold mornings in subalpine meadows, refueling hummingbirds achieve net energy gain despite the high energetic costs of thermoregulation and flight. In doing so, they sustain the highest known time-averaged metabolic rates among vertebrates. However, low sucrose concentrations, provided in volumes large enough to allow the maintenance of energy balance at low temperature, result in energy deficit and mass loss. The problem of disposing of dietary water at low ambient temperature when intake rates are elevated suggests that the kidneys may be involved in establishing the upper limit to intake rates and, therefore, maximum sustained metabolic rates. It is suggested that hummingbird behaviour and metabolism have coevolved to maximize net energy gain. Further, the energetics of hummingbird thermoregulation and flight may have influenced the evolution of sucrose content in floral nectar. 相似文献
45.
Class A scavenger receptors mediate cell adhesion via activation of G(i/o) and formation of focal adhesion complexes 总被引:2,自引:0,他引:2
Class A macrophage scavenger receptors (SR-A) are multifunctional receptors with roles in modified lipoprotein uptake, innate immunity, and macrophage adhesion. Our previous studies conducted in mouse peritoneal macrophages demonstrated that pertussis toxin (PTX) mediated inhibition of G(i/o) attenuated SR-A-dependent uptake of modified lipoprotein. The finding that SR-A-mediated lipoprotein internalization was PTX-sensitive led us to hypothesize that SR-A-mediated cell adhesion might be similarly regulated by G(i/o)-dependent signaling pathways. To test this hypothesis, SR-A was expressed in HEK cells under inducible control. Relative to HEK cells that lack SR-A, SR-A expressing cells displayed enhanced adhesion to tissue culture dishes. SR-A-mediated adhesion was significantly reduced following PTX treatment and was insensitive to chelating divalent cations with EDTA. SR-A-expressing cells exhibited a distinct cell morphology characterized by fine filopodia-like projections. Both polymerized actin and vinculin were codistributed with SR-A in the filopodia-like projections indicating the formation of focal adhesion complexes. Overall, our results indicate that the ability of SR-A to enhance cell adhesion involves G(i/o) activation and formation of focal adhesion complexes. 相似文献
46.
Rick PD; Hubbard GL; Kitaoka M; Nagaki H; Kinoshita T; Dowd S; Simplaceanu V; Ho C 《Glycobiology》1998,8(6):557-567
The polysaccharide chains of enterobacterial common antigen (ECA) consist
of linear trisaccharide repeat units with the structure -->3)-
alpha-d-Fuc4NAc-(1-->4)-beta-d-ManNAcA-(1-->
4)-alpha-d-GlcNAc-(1-->, where Fuc4NAc is 4-acetamido-4,
6-dideoxy-d-galactose, ManNAcA is N - acetyl-d- mannosaminuronic acid, and
GlcNAc is N -acetyl-d-glucosamine. The major form of ECA (ECAPG) consists
of polysaccharide chains that are believed to be covalently linked to
diacylglycerol through phosphodiester linkage; the phospholipid moiety
functions to anchor molecules in the outer membrane. The ECA trisaccharide
repeat unit is assembled as a polyisoprenyl-linked intermediate which has
been tentatively identified as Fuc4NAc-ManNAcA-GlcNAc-
pyrophosphorylundecaprenol (lipid III). Subsequent chain-elongation
presumably occurs by a block-polymerization mechanism. However, the
identity of the polyisoprenoid carrier-lipid has not been established.
Accordingly, the current studies were conducted in an effort to
structurally characterize the polyisoprenyl lipid-carrier involved in ECA
synthesis. Isolation and characterization of the lipid carrier was
facilitated by the accumulation of a ManNAcA-GlcNAc-
pyrophosphorylpolyisoprenyl lipid (lipid II) in mutants of Salmonella
typhimurium defective in the synthesis of TDP-Fuc4NAc, the donor of Fuc4NAc
residues for ECA synthesis. Analyses of lipid II preparations by fast atom
bombardment tandem mass spectroscopy (FAB-MS/MS) resulted in the
identification of the lipid-carrier as the 55-carbon polyisoprenyl alcohol,
undecaprenol. These analyses also resulted in the identification of a novel
glycolipid which copurified with lipid II. FAB-MS/MS analyses of this
glycolipid revealed its structure to be 1,2-diacyl- sn
-glycero-3-pryophosphoryl-GlcNAc-ManNAcA (DGP- disaccharide). An
examination of purified ECAPGby phosphorus-31 nuclear magnetic resonance
spectroscopy confirmed that the polysaccharide chains are linked to
diacylglycerol through phosphodiester linkage. Thus, DGP-disaccharide does
not appear to be an intermediate in ECAPGsynthesis. Nevertheless, although
the available evidence clearly indicate that lipid II is a precursor of
DGP-disaccharide, the function of this novel glycolipid is not yet known,
and it may be an intermediate in the biosynthesis of a molecule other than
ECAPG.
相似文献
47.
角质形成细胞生长因子(KGF)在喉粘膜良性、癌前及恶性病变中的mRNA水平分析 总被引:1,自引:0,他引:1
利用原位杂交的方法检测KGFmRNA在正常喉粘膜上皮(N)、慢性非特异性炎症(IF)、不典型增生(DYS)及鳞癌(SCC)中的转录水平,探讨KGF在喉粘膜良性及恶性病变中的分布和可能的作用。结果表明,KGFmRNA不仅在间质中的成纤维细胞中表达,少量的炎细胞及血管内皮细胞中亦表达,而且从N、IF、DYS到SCC、KGFmRNA转录水平逐渐增强;上皮细胞及肿瘤性上皮细胞不表达KGFmRNA,KGFmRNA在分化差的SCC周围间质中表达较分化好的SCC周围间质增多。结论:KGF在上皮与间充质细胞的交互作用中发挥着重要的作用,对维持喉粘膜正常结构、代谢及喉癌的发生发展具有重要意义。 相似文献
48.
Natalya Kostandova Luccene Desir Abdel Direny Alaine Knipes Jean Frantz Lemoine Carl Renand Fayette Amy Kirby Katherine Gass 《PLoS neglected tropical diseases》2022,16(1)
BackgroundThe Transmission Assessment Survey (TAS) is a decision-making tool to determine when transmission of lymphatic filariasis is presumed to have reached a level low enough that it cannot be sustained even in the absence of mass drug administration. The survey is applied over geographic areas, called evaluation units (EUs); existing World Health Organization guidelines limit EU size to a population of no more than 2 million people.Methodology/Principal findingsIn 2015, TASs were conducted in 14 small EUs in Haiti. Simulations, using the observed TAS results, were performed to understand the potential programmatic impact had Haiti chosen to form larger EUs. Nine “combination-EUs” were formed by grouping adjacent EUs, and bootstrapping was used to simulate the expected TAS results.When the combination-EUs were comprised of at least one “passing” and one “failing” EU, the majority of these combination-EU would pass the TAS 79% - 100% of the time. Even in the case when both component EUs had failed, the combination-EU was expected to “pass” 11% of the time.Simulations of mini-TAS, a strategy with smaller power and hence smaller sample size than TAS, resulted in more conservative “passing” and “failing” when implemented in original EUs.Conclusions/SignificanceOur results demonstrate the high potential for misclassification when the average prevalence of lymphatic filariasis in the combined areas differs with regards to the TAS threshold. Of particular concern is the risk of “passing” larger EUs that include focal areas where prevalence is high enough to be potentially self-sustaining. Our results reaffirm the approach that Haiti took in forming smaller EUs. Where baseline or monitoring data show a high or heterogeneous prevalence, programs should leverage alternative strategies like mini-TAS in smaller EUs, or consider gathering additional data through spot check sites to advise EU formation. 相似文献
49.
The environmental contaminants methylmercury (MeHg) and mercuric chloride (HgCl2) stimulated the spontaneous release of [3H]noradrenaline ([3H]NA) from hippocampal slices in a time- and concentration-dependent manner. Both MeHg and HgCl2 were similarly potent, with an EC50 of 88.4 microM and 75.9 microM, respectively. The releasing effects of MeHg and HgCl2 increased in the presence of desipramine, showing that the mechanism does not involve reversal of the transmitter transporter, and were completely blocked by reserpine preincubation, indicating a vesicular origin of [3H]NA release. The voltage-gated Na+ channel blocker tetrodotoxin (TTX) did not affect the response to mercury compounds. [3H]NA release elicited by MeHg was partially dependent on extracellular Ca2+, since it decreased significantly in a Ca2+-free EGTA-containing medium whereas HgCl2 induced a release of [3H]NA independent of extracellular Ca2+. Neither Ca2+-channels blockers, cobalt chloride (CoCl2) and (omega-conotoxin-GVIA, nor the Na+/Ca2+-exchanger inhibitor benzamil reduced MeHg-evoked [3H]NA release. Moreover, thapsigargin or caffeine, endoplasmic reticulum Ca2+-depletors, did not modify metal-evoked [3H]NA release, whereas ruthenium red, which inhibits the mitochondrial Ca2+ transport, decreased the effect of both MeHg and HgCl2. All these data indicate that, in hippocampal slices, mercury compounds release [3H]NA from the vesicular pool by a mechanism involving Ca2+ mobilization from mitochondrial stores. 相似文献
50.