Analysis of mouse
Tcr genes has previously defined at least five different
Tcra-V haplotypes among inbred strains of mice. For mice of the
Tcra-V
b
haplotype, including C57BL/10 (B10), T-cell expression of the
Tcra-V11 gene subfamily can be detected with a monoclonal antibody, 1.F2. In the course of further characterizing the specificity of 1.F2, we found that it fails to recognize
Tcra-V11-expressing T-cell hybrids derived from the B10 congenic strain, B10.A(18R)/SgIcr. Moreover, staining analysis indicated that the Va11 epitope recognized by 1.F2 is not expressed by peripheral T cells from several different B10.A(18R) colonies with the exception of that at the Research Institute of Scripps Clinic. Nucleotide sequences were determined for cDNA representing rearranged
Tcra-V11 genes from two independent, B10.A(18R)/SgIcr derived T-cell hybrids. The two
Tcra-V11 gene segments were identical and the predicted amino acid sequence differed by at least five residues from
Tcra-V11 sequences previously obtained from B10.A mice. Southern blot analysis of restriction fragment length polymorphisms (RFLP) associated with
Tcra-V11, as well as
Tcra-V
1
, subfamily genes revealed that the B10.A(18R) mouse has inherited
Tcra-V
a
alleles rather than the expected
Tcra-V
b
alleles from the B10 strain. RFLP analysis of the
Rib-1 locus, located in close proximity to the
Tcra locus on chromosome 14, showed that B10.A(18R) carries the
Rib-1
b
allele from B10. These results indicate that the B10.A(18R) mouse has inherited a recombinant chromosome 14 with a recombination event having occured between the
Rib-1 locus and the
Tcra-V
a
gene subfamilies examined. Inheritance of
Tcra-V
a
alleles in B10.A(18R) probably originated from strain 129/J which breeding records show was used in the first cross with B10.A in the production of B10.A(18R) and which we found exhibits
Tcra-V11
a
RFLPs.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers M55634 and M55635.
Address correspondence and offprint requests to: P. B. Nakajima.
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