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61.
62.
G J Hanson J S Baran H S Lowrie M A Russell S J Sarussi K Williams M Babler S E Bittner S E Papaioannou P C Yang 《Biochemical and biophysical research communications》1989,160(1):1-5
We prepared a new series of renin inhibitors based on dipeptide glycols, replacing the P4-P3 subsites with an O-(N-morpholinocarbonyl)-3-L-phenyllactic acid residue. This modification proved bioisosteric with Boc-L-phenylalanine, giving rise to highly potent human renin inhibitors (1-5 nM), e.g., SC-46944 (IC50 = 5 nM). Moreover, this change produced compounds that are orally efficacious in reducing plasma renin activity in salt-depleted marmosets. 相似文献
63.
Papaioannou M Chakarova CF Prescott DC Waseem N Theis T Lopez I Gill B Koenekoop RK Bhattacharya SS 《Human genetics》2005,118(3-4):501-503
Retinitis pigmentosa (RP) is a debilitating disease of the retina affecting ∼1.5 million people worldwide. RP shows remarkable heterogeneity both clinically and genetically, with more than 40 genetic loci implicated, 12 of which account for the autosomal dominant form (adRP) of inheritance. We have recently identified a French Canadian family that presents with early onset adRP. After exclusion of all known loci for adRP, a genome-wide search established firm linkage with a marker from the short arm of chromosome 9 (LOD score of 6.3 at recombination fraction θ=0). The linked region is flanked by markers D9S285 and D9S1874, corresponding to a genetic distance of 31 cM, in the region 9p22-p13. 相似文献
64.
The oral cavity offers a range of different niches where periodontopathogens can adhere and survive (e.g. the mucosa, the tongue, the tonsils, the saliva and all intra-oral hard surfaces such as teeth, dentures, oral implants). Transmission of bacteria from one niche to another is likely to occur. Recent studies, for example, illustrated that initially sterile abutments of oral implants were rapidly colonized by bacteria from the subgingival environment around teeth. This transmission of bacteria can occur spontaneously via the saliva, or by means of oral hygiene aids and/or dental instruments. Such an intra-oral transmission, if it occurs at a high speed, could jeopardize the outcome of periodontal therapy. To overcome a bacterial transmission, a 'one-stage full-mouth disinfection' was recently introduced for the treatment of periodontal infections. This new treatment strategy resulted in significant clinical and microbiological improvements when compared with the standard sequential treatment. 相似文献
65.
E N Vithana L Abu-Safieh M J Allen A Carey M Papaioannou C Chakarova M Al-Maghtheh N D Ebenezer C Willis A T Moore A C Bird D M Hunt S S Bhattacharya 《Molecular cell》2001,8(2):375-381
We report mutations in a gene (PRPF31) homologous to Saccharomyces cerevisiae pre-mRNA splicing gene PRP31 in families with autosomal dominant retinitis pigmentosa linked to chromosome 19q13.4 (RP11; MIM 600138). A positional cloning approach supported by bioinformatics identified PRPF31 comprising 14 exons and encoding a protein of 499 amino acids. The level of sequence identity to the yeast PRP31 gene indicates that PRPF31 is also likely to be involved in pre-mRNA splicing. Mutations that include missense substitutions, deletions, and insertions have been identified in four RP11-linked families and three sporadic RP cases. The identification of mutations in a pre-mRNA splicing gene implicates defects in the splicing process as a novel mechanism of photoreceptor degeneration. 相似文献
66.
J Jain E A Nalefski P G McCaffrey R S Johnson B M Spiegelman V Papaioannou A Rao 《Molecular and cellular biology》1994,14(3):1566-1574
67.
S. Knodle S. Anderson S. Papaioannou 《In vitro cellular & developmental biology. Plant》1986,22(1):23-27
Summary Rabbit aortic smooth muscle cells were prepared by enzymatic digestion of the aortic smooth muscle layer. The cells were subcultured
up to Passage 22 starting from a cryogenically preserved stock (approximately 1010cells, Passage 8) and characterized morphologically and for45Ca++ uptake. Microscopically the cells demonstrated the characteristics of vascular smooth muscle cells.45Ca++ uptake by the cells plated on tissue culture flasks (25 cm2) was determined at 25°C in physiological salt solution (PSS) containing45Ca++ in low (5 mM) or high (50mM) KCl concentrations. At the end of the incubation period (0 to 30 min), PSS was aspirated and the cells quickly washed, digested
with 0.5N NaOH, and counted for45Ca++. High K+ increased the45Ca++ uptake by 100% or more compared to the low K+ uptake of45Ca++. This K+-induced45Ca++ uptake was eliminated in osmotically shocked cells, and inhibited by nifedipine, verapamil, and diltiazem, in a dose-dependent
manner. The extent of45Ca++ uptake and the inhibitory activity of nifedipine were retained up to Passage 22. It is concluded that the developed methodology
for scaled-up cultures of rabbit aortic smooth muscle cells provides morphologically intact and biochemically functioning
cells suitable for calcium channel studies. 相似文献
68.
Two diploid embryonal carcinoma (EC) cell lines, P10 and P19, differ in their response to the embryonic environment. P10 produces mostly normal chimeras following injection into blastocysts, whereas P19 produces mostly abnormal chimeras. In this study, P10 cells were aggregated with morulae, and all resulting fetuses were chimeric with very large contributions from the EC cells. However, all embryos were abnormal. Following aggregation of P19 cells with morulae, very few embryos were recovered and they were all non-chimeric. Both P10 and P19 were capable of forming functional gap junctions with morula cells and with the ICM of the blastocyst but not with trophoblast, showing that differences in the ability to make junctional contact with the embryo cannot explain the differences between the two cell lines. 相似文献
69.
Lisa M. Lix Xiuyun Wu Wilma Hopman Nancy Mayo Tolulope T. Sajobi Juxin Liu Jerilynn C. Prior Alexandra Papaioannou Robert G. Josse Tanveer E. Towheed K. Shawn Davison Richard Sawatzky 《PloS one》2016,11(3)
Background
Self-reported health status measures, like the Short Form 36-item Health Survey (SF-36), can provide rich information about the overall health of a population and its components, such as physical, mental, and social health. However, differential item functioning (DIF), which arises when population sub-groups with the same underlying (i.e., latent) level of health have different measured item response probabilities, may compromise the comparability of these measures. The purpose of this study was to test for DIF on the SF-36 physical functioning (PF) and mental health (MH) sub-scale items in a Canadian population-based sample.Methods
Study data were from the prospective Canadian Multicentre Osteoporosis Study (CaMos), which collected baseline data in 1996–1997. DIF was tested using a multiple indicators multiple causes (MIMIC) method. Confirmatory factor analysis defined the latent variable measurement model for the item responses and latent variable regression with demographic and health status covariates (i.e., sex, age group, body weight, self-perceived general health) produced estimates of the magnitude of DIF effects.Results
The CaMos cohort consisted of 9423 respondents; 69.4% were female and 51.7% were less than 65 years. Eight of 10 items on the PF sub-scale and four of five items on the MH sub-scale exhibited DIF. Large DIF effects were observed on PF sub-scale items about vigorous and moderate activities, lifting and carrying groceries, walking one block, and bathing or dressing. On the MH sub-scale items, all DIF effects were small or moderate in size.Conclusions
SF-36 PF and MH sub-scale scores were not comparable across population sub-groups defined by demographic and health status variables due to the effects of DIF, although the magnitude of this bias was not large for most items. We recommend testing and adjusting for DIF to ensure comparability of the SF-36 in population-based investigations. 相似文献70.
Cynthia M Balion Carolyn Benson Parminder S Raina Alexandra Papaioannou Christopher Patterson Afisi S Ismaila 《BMC neurology》2007,7(1):38