全文获取类型
收费全文 | 3298篇 |
免费 | 354篇 |
国内免费 | 2篇 |
出版年
2023年 | 12篇 |
2022年 | 25篇 |
2021年 | 78篇 |
2020年 | 54篇 |
2019年 | 71篇 |
2018年 | 70篇 |
2017年 | 62篇 |
2016年 | 112篇 |
2015年 | 152篇 |
2014年 | 171篇 |
2013年 | 206篇 |
2012年 | 264篇 |
2011年 | 267篇 |
2010年 | 135篇 |
2009年 | 139篇 |
2008年 | 183篇 |
2007年 | 176篇 |
2006年 | 156篇 |
2005年 | 156篇 |
2004年 | 147篇 |
2003年 | 139篇 |
2002年 | 131篇 |
2001年 | 58篇 |
2000年 | 58篇 |
1999年 | 47篇 |
1998年 | 43篇 |
1997年 | 31篇 |
1996年 | 22篇 |
1995年 | 20篇 |
1994年 | 14篇 |
1993年 | 12篇 |
1992年 | 27篇 |
1991年 | 19篇 |
1990年 | 20篇 |
1989年 | 21篇 |
1988年 | 24篇 |
1987年 | 20篇 |
1986年 | 17篇 |
1985年 | 20篇 |
1984年 | 19篇 |
1983年 | 19篇 |
1982年 | 11篇 |
1980年 | 11篇 |
1979年 | 38篇 |
1978年 | 27篇 |
1977年 | 23篇 |
1976年 | 23篇 |
1975年 | 21篇 |
1973年 | 9篇 |
1971年 | 11篇 |
排序方式: 共有3654条查询结果,搜索用时 547 毫秒
991.
Does sediment resuspension have persistent effects on phytoplankton? Experimental studies in three shallow lakes 总被引:10,自引:0,他引:10
1. The persistence of effects of sediment resuspension on chlorophyll a , phytoplankton production and plant nutrients was examined by artificially resuspending sediment in enclosures in three shallow lakes and monitoring concentrations for 4–8 days. Realism was assessed by relating initial suspended solids concentrations to those observed during natural wind events.
2. Positive effects on the phytoplankton, persisting for at least 4–6 days were detected in eight of the nine experiments, and similar effects on nutrient concentrations were also common, although suspended solids had normally returned to control levels within 24 h
3. The phytoplankton during the periods of persistence was normally dominated by planktonic rather than benthic/meroplanktonic genera.
4. Several of the positive responses appeared to be associated with relief of nitrogen deficiency in the algae.
5. Persistent effects from previous resuspension events may cause baseline concentrations of phytoplankton and nutrients in shallow lakes to be overestimated and the effects of resuspension on phytoplankton and nutrients to be underestimated 相似文献
2. Positive effects on the phytoplankton, persisting for at least 4–6 days were detected in eight of the nine experiments, and similar effects on nutrient concentrations were also common, although suspended solids had normally returned to control levels within 24 h
3. The phytoplankton during the periods of persistence was normally dominated by planktonic rather than benthic/meroplanktonic genera.
4. Several of the positive responses appeared to be associated with relief of nitrogen deficiency in the algae.
5. Persistent effects from previous resuspension events may cause baseline concentrations of phytoplankton and nutrients in shallow lakes to be overestimated and the effects of resuspension on phytoplankton and nutrients to be underestimated 相似文献
992.
993.
994.
995.
Previous studies have shown that a variety of mammalian cell types, including macrophages, contain small amounts of redox-active iron in their lysosomes. Increases in the level of this iron pool predispose the cell to oxidative stress. Limiting the availability of intralysosomal redox-active iron could therefore represent potential cytoprotection for cells under oxidative stress.
In the present study we have shown that an initial 6 h exposure of J774 macrophages to 30 μM iron, added to the culture medium as FeCl3, increased the lysosomal iron content and their sensitivity to H2O2-induced (0.25 mM for 30 min) oxidative stress. Over time (24-72 h), however, the cells were desensitized to the cytotoxic effects of H2O2; most likely as a consequence of both lysosomal iron exocytosis and of ferritin synthesis (demonstrated by atomic absorption spectrophotometry, autometallography, and immunohistochemistry). When the cells were exposed to a second dose of iron, their lysosomal content of iron increased again but the cells became no further sensitized to the cytotoxic effects of H2O2. Using the lysosomotropic weak base, acridine orange, we demonstrated that after the second exposure to iron and H2O2, lysosomes remained intact and were no different from control cells which were exposed to H2O2 but not iron.
These data suggest that the initial induction of ferritin synthesis leads to enrichment of lysosomes with ferritin via autophagocytosis. This limits the redox-availability of intralysosomal iron and, in turn, decreases the cells' sensitivity to oxidative stress. These in vitro observations could also explain why cells under pathological conditions, such as haemochromatosis, are apparently able to withstand high iron concentrations for some time in vivo. 相似文献
In the present study we have shown that an initial 6 h exposure of J774 macrophages to 30 μM iron, added to the culture medium as FeCl3, increased the lysosomal iron content and their sensitivity to H2O2-induced (0.25 mM for 30 min) oxidative stress. Over time (24-72 h), however, the cells were desensitized to the cytotoxic effects of H2O2; most likely as a consequence of both lysosomal iron exocytosis and of ferritin synthesis (demonstrated by atomic absorption spectrophotometry, autometallography, and immunohistochemistry). When the cells were exposed to a second dose of iron, their lysosomal content of iron increased again but the cells became no further sensitized to the cytotoxic effects of H2O2. Using the lysosomotropic weak base, acridine orange, we demonstrated that after the second exposure to iron and H2O2, lysosomes remained intact and were no different from control cells which were exposed to H2O2 but not iron.
These data suggest that the initial induction of ferritin synthesis leads to enrichment of lysosomes with ferritin via autophagocytosis. This limits the redox-availability of intralysosomal iron and, in turn, decreases the cells' sensitivity to oxidative stress. These in vitro observations could also explain why cells under pathological conditions, such as haemochromatosis, are apparently able to withstand high iron concentrations for some time in vivo. 相似文献
996.
Kits for the detection of some bacterial food poisoning toxins: problems, pitfalls and benefits 总被引:3,自引:0,他引:3
Brett 《Journal of applied microbiology》1998,84(S1):110S-118S
997.
Despite the importance of the soil seed bank in tropical forest regeneration, little is known about spatial variability in species composition and abundance of seeds stored in the soil. To develop sampling methods for comparative studies, we examined species richness, spatial variation, and abundance of germinants from the soil seed bank in a 16 year old secondary, tropical wet forest at La Selva Biological Station, Costa Rica. Surface soil (10 cm deep, 4.7 cm diameter) was collected at the intersection points of a gridded 1 ha plot (10 × 10-m grid, 121 samples) and in a nested 100 m2 subplot (2 × 2-m grid, 36 samples). The 1 ha plot had a density of 4535 seeds/m2 with 34 species observed. Based on a series of 100 randomized species accumulation curves, a Michaelis-Menten fit predicted a mean species richness of 36.3 species; the number of observed species was close to the predicted asymptote. A nonparametric, first-order jackknife species richness estimator predicted a species richness of 37.0 species. Eighty-five and 95 percent of the observed species richness is contained, on average, within 41 and 74 pooled samples, respectively. Within the 100 m2 nested subplot, a density of 5476 seeds/m2 was observed, comprising 26 species with an estimated species richness (Michaelis-Menten fit) of 29.1 species. The jackknife species richness estimator predicted a species richness of 36.7 species. For species richness and abundance of both plots, spatial autocorrelation statistics (Moran's I) were not significantly different from zero at lag distances from 2 to 100 m, indicating a random distribution at these spatial scales. For this site, accurate estimates of species composition depend upon the number of samples collected as well as the spatial distribution of sampling effort. Many small samples distributed over a large area provide greater accuracy and precision for estimating species richness of the soil seed bank. 相似文献
998.
Objective: To determine whether preventive treatment for tuberculosis in adults infected with HIV reduces the frequency of tuberculosis and overall mortality. Design: Systematic review and data synthesis of randomised placebo controlled trials. Main outcome measures: Active tuberculosis, mortality, and adverse drug reaction requiring cessation of the study regimen. Outcomes stratified by status of purified protein derivative skin test. Results: Four trials comprising 4055 adults from Haiti, Kenya, the United States, and Uganda were included. All compared isoniazid (6-12 months) with placebo, and one trial also compared multidrug treatment for 3 months with placebo. Mean follow up was 15-33 months. Overall, frequency of tuberculosis (relative risk 0.57, 95% confidence interval 0.41 to 0.79) was reduced in those receiving preventive treatment compared with placebo: mortality was not significantly reduced (0.93, 0.83 to 1.05). In subjects positive for purified protein derivative receiving preventive treatment, the risk of tuberculosis was reduced substantially (0.32, 0.19 to 0.51) and the risk of death was reduced moderately (0.73, 0.57 to 0.95) compared with those taking placebo. In adults negative for purified protein derivative receiving preventive treatment, the risk of tuberculosis (0.82, 0.50 to 1.36) and the risk of death (1.02, 0.89 to 1.17) were not reduced significantly. Adverse drug reactions were more frequent, but not significantly so, in patients receiving drug compared with placebo (1.45, 0.98 to 2.14). Conclusions: Preventive treatment given for 3-12 months protects against tuberculosis in adults infected with HIV, at least in the short to medium term. Protection is greatest in subjects positive for purified protein derivative, in whom death is also less frequent. Long term benefits remain to be shown.
Key messages
- One third of the world’s population is infected with Mycobacterium tuberculosis
- People infected with HIV are at much increased risk of developing active tuberculosis
- Short term preventive drug treatment given to people infected with HIV reduces the occurrence of active tuberculosis
- The benefit is greatest in people with latent infection, as shown by a positive skin test for tuberculosis, and this group also exhibits a survival benefit
999.
Summary A unique form of cell division is reported for the cellsKomma caudata andCryptomonas ovata (Cryptophyceae). During cytokinesis, the posterior tail-like region of each daughter cell develops from the anterior region of the parental cell. This process, termed pole reversal, involves a major realignment in overall cell polarity as well as alterations to cytoplasmic and surface components. Pole reversal may be a consequence of flagellar apparatus transformation and reorientation during division, and pole reversal may facilitate the development of the asymmetric cell shape in daughter cells. 相似文献
1000.
Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active on IL2 as on beta-globin mRNA. Pancreatic RNase, by contrast, does not show a significant preference for IL2 mRNA. Neither 5' capping, nor polyadenylation of the substrate mRNAs affects their degradation by the IL2-selective mRNase, whose activity is optimal in 0.5 mM Mg++ and 100 mM potassium acetate. The mRNase behaves like a protein of molecular weight 60-70,000 on gel chromatography, and is unusual in that it is insensitive to placental RNase inhibitor (RNasin). The mRNase cleaves IL2 mRNA at a small number of sites in the coding region, and IL2 mRNA containing only the coding region and 36 nucleotides of the 3'-noncoding region competes efficiently with full-length IL2 mRNA for the mRNase, whereas beta-globin mRNA does not. 相似文献