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71.
Montserrat Barquín María J. González Garmendia Elena Pinilla María R. Torres 《Inorganica chimica acta》2009,362(7):2334-12
By the reactions of Cu(OAc)2 · H2O and Cu(HCOO)2 · 4H2O with 2-(aminomethyl)pyridine in different proportions, the compounds Cu(OAc)2(2-amp) (1), Cu(HCOO)2(2-amp) (2), Cu(HCOO)2(2-amp)1/2 (5), Cu(OAc)2(2-amp)2 · H2O (6) and Cu(HCOO)2(2-amp)2 · H2O (7) were obtained. In 1 the copper shows an elongated rhombic octahedral stereochemistry determined by a 2-amp molecule and two asymmetrical bidentate acetate groups. The hydrogen bonds between the NH2 groups and O atoms yield to the formation of a double chain. Compound 2 instead consists in monodimensional chains of Cu(2-amp)(HCOO) units, with monodentate formate groups, linked by syn-anti bridging formate groups. Sheets are formed by hydrogen bonds between the chains. By crystallization of a solution of 6 in chloroform, CuCl2(2-amp)2 (3) was obtained. It presents a highly distorted square pyramidal geometry around the copper atom. The sheets, formed by the hydrogen bonds between NH2 and Cl, are interpenetrated and shows π stacking. Magnetic properties and EPR spectra for these new compounds have been studied. Also the magnetic behaviour of Cu(OAc)2(2-amp)1/2 (4) is described. 相似文献
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75.
Frasca S Linfert DR Tsongalis GJ Gorton TS Garmendia AE Hedrick RP West AB Van Kruiningen HJ 《Diseases of aquatic organisms》1999,35(3):221-233
During seasonal epizootics of neurologic disease and mass mortality in the summers of 1992, 1993 and 1994 on a sea-farm in Ireland, Atlantic salmon Salmo salar smolts suffered from encephalitis associated with infection by a neurotropic parasite. Based on ultrastructural studies, this neurotropic parasite was identified as an intercellular presporogonic multicellular developmental stage of a histozoic myxosporean, possibly a Myxobolus species. In order to generate sequence data for phylogenetic comparisons to substantiate the present morphological identification of this myxosporean in the absence of detectable sporogony, polymerase chain reaction (PCR), Southern blot hybridization, dideoxynucleotide chain-termination DNA sequencing, and in situ hybridization (ISH) were used in concert to characterize segments of the small subunit ribosomal RNA (SSU rRNA) gene. Oligonucleotide primers were created from sequences of the SSU rRNA gene of M. cerebralis and were employed in PCR experiments using DNA extracted from formalin-fixed paraffin-embedded tissue sections of brains from Atlantic salmon smolts in which the myxosporean had been detected by light microscopy. Five segments of the SSU rRNA gene of the myxosporean, ranging in length from 187 to 287 base pairs, were amplified, detected by hybridization with sequence-specific probes, and sequenced. Consensus sequences from these segments were aligned to create a partial sequence of the SSU rRNA gene of the myxosporean. Assessments of sequence identity were made between this partial sequence and sequences of SSU rRNA genes from 7 myxosporeans, including Ceratomyxa shasta, Henneguya doori, M. arcticus, M. cerebralis, M. insidiosus, M. neurobius, and M. squamalis. The partial SSU rRNA gene sequence from the myxosporean had more sequence identity with SSU rRNA gene sequences from neurotropic and myotropic species of Myxobolus than to those from epitheliotropic species of Myxobolus or Henneguya, or the enterotropic species of Ceratomyxa, and was identical to regions of the SSU rRNA gene of M. cerebralis. Digoxigenin-labeled oligonucleotide DNA probes complementary to multiple segments of the SSU rRNA gene of M. cerebralis hybridized with DNA of the parasite in histologic sections of brain in ISH experiments, demonstrating definitively that the segments of genome amplified were from the organisms identified by histology and ultrastructural analysis. Based on sequence data derived entirely from genetic material of extrasporogonic stages, the SSU rDNA sequence identity discovered in this study supports the hypothesis that the myxosporean associated with encephalitis of farmed Atlantic salmon smolts is a neurotropic species of the genus Myxobolus, with sequences identical to those of M. cerebralis. 相似文献
76.
Idoia?Garmendia Yolanda?Gogorcena Iker?Aranjuelo Nieves?GoicoecheaEmail author 《Journal of Plant Growth Regulation》2017,36(4):855-867
A greater understanding of how climate change will affect crop photosynthetic performance has been described as a target goal to improve yield potential. Other concomitant stressors can reduce the positive effect of elevated atmospheric CO2 on wheat yield. Arbuscular mycorrhizal fungi (AMF) are symbiotic fungi predicted to be important in defining plant responses to rising atmospheric CO2, but their role in response to global climatic change is still poorly understood. This study aimed to assess if increased atmospheric CO2 interacting with drought can modify the effects of mycorrhizal symbiosis on flag leaf physiology in winter wheat. The study was performed in climate-controlled greenhouses with ambient (400 ppm, ACO2) or elevated (700 ppm, ECO2) CO2 concentrations in the air. Within each greenhouse half of the plants were inoculated with Rhizophagus intraradices. When ear emergence began, half of the plants from each mycorrhizal and CO2 treatment were subjected to terminal drought. At ACO2 AMF improved the photochemistry efficiency of PSII compared with non-mycorrhizal plants, irrespective of irrigation regime. Mycorrhizal wheat accumulated more fructan than non-mycorrhizal plants under optimal irrigation. The level of proline in the flag leaf increased only in mycorrhizal wheat after applying drought. Mycorrhizal association avoided photosynthetic acclimation under ECO2. However, nitrogen availability to flag leaves in mycorrhizal plants was lower under ECO2 than at ACO2. Results suggest that the mechanisms underlying the interactions between mycorrhizal association and atmospheric CO2 concentration can be crucial for the benefits that this symbiosis can provide to wheat plants undergoing water deficit. 相似文献
77.
Vesa?P?Hyt?nen Juha?AE?M??tt? Heidi?Kidron Katrin?K?Halling Jarno?H?rh? Tuomas?Kulomaa Thomas?KM?Nyholm Mark?S?Johnson Tiina?A?Salminen Markku?S?Kulomaa Tomi?T?AirenneEmail author 《BMC biotechnology》2005,5(1):28
Background
The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in numerous modern life science applications. Recently, the AVR genes have been expressed as recombinant proteins, which have shown different biotin-binding properties as compared to avidin. 相似文献78.
79.
M Kale R Ramsey-Goldman S Bernatsky MB Urowitz D Gladman PR Fortin M Petri E Yelin S Manzi S Edworthy O Nived S-C Bae D Isenberg A Rahman JG Hanly C Gordon S Jacobsen E Ginzler DJ Wallace GS Alarcón MA Dooley L Gottesman K Steinsson A Zoma J-L Senécal S Barr G Sturfelt L Dreyer L Criswell J Sibley JL Lee AE Clarke 《Arthritis research & therapy》2012,14(Z3):A15
80.
Catalina March Verónica Regueiro Enrique Llobet David Moranta Pau Morey Junkal Garmendia José A. Bengoechea 《PloS one》2010,5(4)
Infected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including β-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-κB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance. 相似文献