全文获取类型
收费全文 | 1300篇 |
免费 | 57篇 |
出版年
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 39篇 |
2020年 | 16篇 |
2019年 | 16篇 |
2018年 | 32篇 |
2017年 | 30篇 |
2016年 | 43篇 |
2015年 | 54篇 |
2014年 | 68篇 |
2013年 | 102篇 |
2012年 | 100篇 |
2011年 | 89篇 |
2010年 | 50篇 |
2009年 | 42篇 |
2008年 | 54篇 |
2007年 | 47篇 |
2006年 | 53篇 |
2005年 | 36篇 |
2004年 | 44篇 |
2003年 | 32篇 |
2002年 | 16篇 |
2001年 | 17篇 |
2000年 | 22篇 |
1999年 | 21篇 |
1997年 | 10篇 |
1996年 | 7篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 16篇 |
1991年 | 14篇 |
1990年 | 17篇 |
1989年 | 21篇 |
1988年 | 21篇 |
1987年 | 19篇 |
1986年 | 12篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1979年 | 18篇 |
1978年 | 7篇 |
1976年 | 12篇 |
1974年 | 5篇 |
1973年 | 10篇 |
1972年 | 14篇 |
1971年 | 5篇 |
1970年 | 6篇 |
排序方式: 共有1357条查询结果,搜索用时 281 毫秒
31.
Protein production by Aspergillus terreus GN1 grown on 1.0% alkali-treated bagasse was studied under various cultural conditions. The maximum biomass protein content of 20.1% and protein recovery of 11.2% was obtained with an initial pH of 4.0, with 1/5 (v/v) inoculum in continuously shaken cultures grown for seven days. Protein content of the alkali-treated bagasse was 3.0%. Highest crude protein percent also corresponded with highest carboxymethyl cellulase and filter paper enzyme activities. 相似文献
32.
33.
Yadav Jyoti Verma Anoop Kumar Ahmad Md. Kaleem Garg Ravindra K. Shiuli Mahdi Abbas Ali Srivastava Shrikant 《Molecular biology reports》2021,48(4):3245-3252
Molecular Biology Reports - Alzheimer's disease is a common neurodegenerative disease in the elderly population and a leading cause of dementia. Genetics and environmental risk factors were... 相似文献
34.
Mannan Ashi Garg Nikhil Singh Thakur Gurjeet Kang Harmeet Kaur 《Neurochemical research》2021,46(11):2800-2831
Neurochemical Research - Cerebral ischemic injury is a leading cause of death and long-term disability throughout the world. Peroxisome proliferator-activated receptor gamma (PPAR-?) is a... 相似文献
35.
Parie Garg Peter M. J. Burgers 《Critical reviews in biochemistry and molecular biology》2013,48(2):115-128
AbstractThree DNA polymerases are thought to function at the eukaryotic DNA replication fork. Currently, a coherent model has been derived for the composition and activities of the lagging strand machinery. RNA-DNA primers are initiated by DNA polymerase α -primase. Loading of the proliferating cell nuclear antigen, PCNA, dissociates DNA polymerase α and recruits DNA polymerase δ and the flap endonuclease FEN1 for elongation and in preparation for its requirement during maturation, respectively. Nick translation by the strand displacement action of DNA polymerase δ, coupled with the nuclease action of FEN1, results in processive RNA degradation until a proper DNA nick is reached for closure by DNA ligase I. In the event of excessive strand displacement synthesis, other factors, such as the Dna2 nuclease/helicase, are required to trim excess flaps. Paradoxically, the composition and activity of the much simpler leading strand machinery has not been clearly established. The burden of evidence suggests that DNA polymerase ε normally replicates this strand, but under conditions of dysfunction, DNA polymerase δ may substitute. 相似文献
36.
Pedicularis zeylanica subsp. anamallyensis T. Husain & A. Garg, a new subspecies of Nilgiri lousewort from the Western Ghats of south India, is described and illustrated. 相似文献
37.
Targeting drug formulations to specific tissues and releasing the bioactive content in response to a certain stimuli remains a significant challenge in the field of biomedical science. We have developed a nanovehicle that can be used to deliver “drugs” to “specific” tissues. For this, we have simultaneously modified the surface of the nanovehicle with “drugs” and “tissue-specific ligands”. The “tissue-specific ligands” will target the nanovehicle to the correct tissue and release the “drug” of interest in response to specific stimuli. We have synthesised a “lactose surface-modified gold nanovehicle” to target liver cells and release the model fluorescent drug (coumarin derivative) in response to the differential glutathione concentration (between blood plasma and liver cells). Lactose is used as the liver-specific targeting ligand given the abundance of l-galactose receptors in hepatic cells. The coumarin derivative is used as a fluorescent tag as well as a linker for the attachment of various biologically relevant molecules. The model delivery system is compatible with a host of different ligands and hence could be used to target other tissues as well in future. The synthesised nanovehicle was found to be non-toxic to cultured human cell lines even at elevated non-physiological concentrations as high as 100 μg/mL. We discover that the synthesised gold-based nanovehicle shows considerable stability at low extracellular glutathione concentrations; however coumarin is selectively released at high hepatic glutathione concentration. 相似文献
38.
39.
40.
Stefanie Bunse Sakshi Garg Stephan Junek Dirk Vogel Nariman Ansari Ernst H. K. Stelzer Erin Schuman 《PloS one》2013,8(12)
Cadherins, Ca2+-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca2+-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca2+-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca2+-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins. 相似文献