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31.
By means of starch gel electrophoresis and specific staining, molecular forms of aspartate aminotransferase and soluble esterases have been investigated in liver extracts of individuals from a population of Zenaida auriculata. Four alleles at the locus corresponding to soluble or cytoplasmic aspartate aminotransferase have been demonstrated. Distribution of phenotypes in the population is in perfect agreement with that expected according to the Hardy - Weinberg law. A large incidence of variants of two fractions of esterases has been found. This variability is, with all probability, genetically determined. The importance of this type of studies to assess genetic structure and dynamics of animal population is emphasized. 相似文献
32.
Paola Lax Juan C. Rondan Dueñas Cristina N. Gardenal Marcelo E. Doucet 《Zoologica scripta》2014,43(2):184-192
The plant‐parasitic nematode Nacobbus aberrans sensu lato is an agricultural pest of quarantine importance. Due to the morphometric, physiological and genetic variability observed within the species, there is no agreement on the taxonomy of this nematode. The objective of this study was to analyse the ITS rDNA region and the D2–D3 expansion segments of 28S rDNA in 10 Argentine populations and one from Ecuador and to establish their phylogenetic relationship with other known sequences from South and North America. Phylogenetic trees of the ITS gene showed seven statistically well‐supported clades; the high and significant Fst values obtained among these groups confirmed this partitioning. The Argentine populations here considered were separated into three clades: one comprising a population from the Andean region and two grouping nematodes from lower altitudes. Three other clades were distinguished for South American populations, which included known sequences of individuals from Peru, Bolivia and north of Argentina. The other clade included sequences from Mexico, Ecuador and two Argentine populations of unknown origin. The important degree of genetic divergence observed among Andean populations suggests that the Andes may have played a crucial role in speciation of Nacobbus, which would have originated in this region. Although D2–D3 segments exhibited lower variation, they were useful for establishing phylogenetic relationships among the Argentine populations considered in this work. As there are no other GenBank sequences available for these segments, it was not possible to make comparisons with other populations from South and North America. The considerable genetic differentiation observed in ITS rDNA region among Nacobbus populations showed evidence of cryptic species within the N. aberrans s.l. complex. Integration of morphological and morphometric studies and molecular analyses considering other genes may aid in the identification of species and their phylogenetic relationships within this genus. 相似文献